ANTIMICROBIAL RESISTANCE PROFILING OF ESCHERICHIA COLI ISOLATED FROM RETAILER POULTRY SAMPLES IN AZAD JAMMU AND KASHMIR

Main Article Content

Amina Javed
Sadia Mumtaz
Madiha Khalid
Aisha Siddique
Tasleem Akhtar
Abdul Rauf

Keywords

Antimicrobial Resistance, Multi drug Resistance, Escherichia coli, Poultry samples, Azad Jammu and Kashmir, Pakistan

Abstract

Antimicrobial resistance (AMR) is a serious threat to animal and human health. Globally, a rapid and alarming increase in antimicrobial resistance (AMR) has been reported in past few years. Escherichia coli is one of the major bacterial enteropathogen of public health concern that causes food borne diseases. As a frequent commensal and zoonotic bacterium, E. coli can be a potential candidate as a pathogenic source for dissemination of antimicrobial resistance. Present study was designed to evaluate the magnitude of antimicrobial resistance in E. coli isolated from poultry samples collected from Bagh, Azad Jammu and Kashmir. For this purpose, 120 samples (liver, spleen, intestines and fecal wastes) were collected from different retailer’s shops of chicken meat in district Bagh during the month of December, 2022 to March, 2023 to evaluate the frequency of E. coli in samples. All the collected samples were processed using established microbiological techniques and isolated E. coli were subjected to antimicrobial profiling by disc diffusion method against 15 different antibiotics. Out of 120 collected samples, 65 (54.17%) samples were found to be positive for E.coli. The prevalence of E.coli in different poultry samples ranged from 43.33 to 70%. Antibiogram results showed significant difference in antibiotic resistance pattern against selected antibiotics (p<0.05) and the highest resistance was observed against nalidixic acid (100%) followed by erythromycin, tetracycline, amoxycilin (95.38%), penicillin G (93.84%) and kanamycin (90.77%). Lower level of resistance was observed against colistin-sulphate (16.92%) and cefipeme (9.23%). Whereas, all isolates were found to be susceptible to Cefoxitin. However, none of E.coli isolate was found susceptible to all antimicrobials tested. Furthermore, all the isolated E.coli were found to be multidrug resistant and showed simultaneous resistant against four or more classes of tested antimicrobials. Maximum number of E.coli isolates (n=28, 43.08%) showed resistance against seven different classes of antimicrobial agents. The current study delineated the incidence of multi drug resistant (MDR) E.coli in poultry samples which endorsed the requirement of proper attention towards regular monitoring and surveillance of exploitation of antimicrobials as chemotherapeutic agents in human and veterinary health care sector in Pakistan.

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References

1. Akinbowale, O. L., Peng, H., & Barton, M. D. (2006). Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. Journal of applied microbiology, 100(5), 1103-1113.
2. Amir, M., Riaz, M., Chang, Y. F., Akhtar, S., Yoo, S. H., Sheikh, A. S., & Kashif,M. (2017). Impact of unhygienic conditions during slaughtering and processing on spread of antibiotic resistant Escherichia coli from poultry. Microbiology Research, 8(2), 7330.
3. Amir, M., Riaz, M., Chang, Y. F., Ismail, A., Hameed, A., & Ahsin, M. (2021). Antibiotic resistance in diarrheagenic Escherichia coli isolated from broiler chickens in Pakistan. Journal of food quality and hazards control.
4. Bauer, A. W. M. M., Kirby, W. M. M., & Sherris, J. C. T. (1966). turck, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology, 45(4), 493.
5. Bergey, D. H. (1934). Bergeyʼs manual of determinative bacteriology. The American Journal of the Medical Sciences, 188(2), 282.
6. Chopra, I., & Roberts, M. (2001). Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiology and molecular biology reviews, 65(2), 232-260.
7. CLSI. Performance Standards for Antimicrobial Susceptibility Testing, 32nd ed.; Clinical and Laboratory Standard Institute (CLSI): Wayne, PA USA, 2022; Volume CLSI supplement M100.
8. de Mesquita Souza Saraiva, M., Lim, K., do Monte, D. F. M., Givisiez, P. E. N., Alves, L. B. R., de Freitas Neto, O. C., ... & Gebreyes, W. A. (2022). Antimicrobial resistance in the globalized food chain: A One Health perspective applied to the poultry industry. Brazilian Journal of Microbiology, 1-22.
9. Debas, G., Kibret, M., Biadglegne, F., & Abera, B. (2011). Prevalence and antimicrobial susceptibility patterns of shigella species at Felege Hiwot Referral Hospital, Northwest Ethiopia. Ethiop Med J, 49(3), 249-56.
10. Denli, M., & Demirel, R. (2018). Replacement of antibiotics in poultry diets. CABI Reviews, (2018), 1-9.

11. Diaz-Sanchez, S., Moscoso, S., Solis de los Santos, F., Andino, A., & Hanning, I. (2015). Antibiotic use in poultry: A driving force for organic poultry production. Food Prot. Trends, 35(6), 440-447.
12. Ezenduka, E. V., Ike, O. S., & Anaelom, N. J. (2014). Rapid detection of antimicrobial residues in poultry: A consequence of non-prudent use of antimicrobials. Health, 2014.
13. García, A., Fox, J. G., & Besser, T. E. (2010). Zoonotic enterohemorrhagic Escherichia coli: a One Health perspective. Ilar Journal, 51(3), 221-232.
14. Ge, B., Domesle, K. J., Gaines, S. A., Lam, C., Bodeis Jones, S. M., Yang, Q., ... & McDermott, P. F. (2020). Prevalence and antimicrobial susceptibility of indicator organisms Escherichia coli and Enterococcus spp. isolated from US animal food, 2005–2011. Microorganisms, 8(7), 1048.
15. Global Antibiotic Resistance Partnership—Nepal Working Group. Nepal Public Health Foundation . Situation Analysis and Recommendations: Antibiotic Use and Resistance in Nepal. Nepal Public Health Foundation; Kathmandu, Nepal: 2015.
16. Iram, S., ul Hassan, M., & Khawaja, T. (2020). Characterization and Antimicrobial Resistance of Escherichia coli and Its Serotypes Isolated from Poultry Feed in Relation to Seasons in Karachi, Pakistan. Jundishapur Journal of Health Sciences, 12(4).
17. Kaper, J. B., Nataro, J. P., & Mobley, H. L. (2004). Pathogenic escherichia coli. Nature reviews microbiology, 2(2), 123-140.
18. Kenneth, I. E., Itohan, I. M., Martha, D. J., Gloria, O. O., & Abdulkarim, Y. (2017). Identification and antibiogram profile of bacteria associated with poultry feeds used in Wukari, Taraba State, North East, Nigeria. Agric Biosyst Eng, 2(6), 48-53.
19. Kiiti, R. W., Komba, E. V., Msoffe, P. L., Mshana, S. E., Rweyemamu, M., & Matee, M. I. (2021). Antimicrobial resistance profiles of Escherichia coli isolated from broiler and layer chickens in Arusha and Mwanza, Tanzania. International Journal of Microbiology, 2021, 1-9.
20. Kim, S., Kim, H., Kim, Y., Kim, M., Kwak, H., & Ryu, S. (2020). Antimicrobial resistance of Escherichia coli from retail poultry meats in Korea. Journal of Food Protection, 83(10), 1673-1678.
21. Koju, P., Shrestha, R., Shrestha, A., Tamrakar, S., Rai, A., Shrestha, P., ... & Shakya Shrestha, S. (2022). Antimicrobial resistance in E. coli isolated from chicken cecum samples and factors contributing to antimicrobial resistance in Nepal. Tropical Medicine and Infectious Disease, 7(9), 249.
22. Lammie, S. L., & Hughes, J. M. (2016). Antimicrobial resistance, food safety, and one health: the need for convergence. Annual review of food science and technology, 7, 287-312.
23. Liaqat, Z., Khan, I., Azam, S., Anwar, Y., Althubaiti, E. H., & Maroof, L. (2022). Isolation and molecular characterization of extended spectrum beta lactamase producing Escherichia coli from chicken meat in Pakistan. Plos one, 17(6), e0269194.
24. Magiorakos, A. P., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske,C. G., ... & Monnet, D. L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection, 18(3), 268-281.
25. Majewski, M., Józefiak, A., Kimsa-Furdzik, M., Dziubdziela, L., Hudak-Nowak, M., Wilczyński, J., & Anusz, K. (2021). Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens– clinical submissions. Annals of Agricultural and Environmental Medicine, 28(2), 271.
A. Makarov, D. A., Ivanova, O. E., Karabanov, S. Y., Gergel, M. A., & Pomazkova, V. (2020). Antimicrobial resistance of commensal Escherichia coli from food- producing animals in Russia. Veterinary World, 13(10), 2053.
26. Maqsood, A. (2012). Salmonella prevalence in the poultry feed industry in Pakistan.

27. Mehdi, Y., Létourneau-Montminy, M. P., Gaucher, M. L., Chorfi, Y., Suresh, G., Rouissi, T., ... & Godbout, S. (2018). Use of antibiotics in broiler production: Global impacts and alternatives. Animal nutrition, 4(2), 170-178.
28. Müller, E. E., Ehlers, M. M., & Grabow, W. O. (2001). The occurrence of E. coli O157: H7 in South African water sources intended for direct and indirect human consumption. Water Research, 35(13), 3085-3088.
29. Nawaz, Z., Aslam, B., Zahoor, M. A., Siddique, A. B., Rafique, A., Aslam, R., ... & Ali, S. (2021). Frequency of extended spectrum beta lactamase producing Escherichia coli in fresh and frozen meat.
30. Nedbalcova, K., Bzdil, J., Papouskova, A., Zouharova, M., Matiaskova, K., Stastny, K., ... & Stolar, P. (2023). Pathotypes and Phenotypic Resistance to Antimicrobials of Escherichia coli Isolates from One-Day-Old Chickens. Pathogens, 12(11), 1330.
31. Ngai, D. G., Nyamache, A. K., & Ombori, O. (2021). Prevalence and antimicrobial resistance profiles of Salmonella species and Escherichia coli isolates from poultry feeds in Ruiru Sub-County, Kenya. BMC research notes, 14(1), 1-6.
32. Olorunmola, F. O., Kolawole, D. O., & Lamikanra, A. (2013). Antibiotic resistance and virulence properties in Escherichia coli strains from cases of urinary tract infections. African journal of infectious diseases, 7(1), 1-7.
33. O'Neill, J. (2016). Tackling drug-resistant infections globally: final report and recommendations.
34. Paredes, R., Damme, M., Mantilla, J., Castellanos, L. R., Clavijo, V., Celis, Y., ... & Jeyashree, K. (2023). Prevalence and antimicrobial resistance of Escherichia coli and Salmonella spp. in animal feed in Colombia. Revista Panamericana de Salud Pública, 47, e57.
35. Pormohammad, A., Nasiri, M. J., & Azimi, T. (2019). Prevalence of antibiotic resistance in Escherichia coli strains simultaneously isolated from humans, animals, food, and the environment: a systematic review and meta- analysis. Infection and drug resistance, 1181-1197.
36. Rafique, M., Potter, R. F., Ferreiro, A., Wallace, M. A., Rahim, A., Ali Malik, A.,... & Dantas, G. (2020). Genomic characterization of antibiotic resistant Escherichia coli isolated from domestic chickens in Pakistan. Frontiers in microbiology, 10, 3052.Robinson, T. P., Bu, D. P., Carrique-Mas, J., Fèvre, E. M., Gilbert, M., Grace, D.,... & Woolhouse, M. E. (2016). Antibiotic resistance is the quintessential One Health issue. Transactions of the Royal Society of Tropical Medicine and Hygiene, 110(7), 377-380.
37. Saeed, M. A., Saqlain, M., Waheed, U., Ehtisham-ul-Haque, S., Khan, A. U., Rehman, A. U., ... & El-Adawy, H. (2023). Cross-Sectional Study for Detection and Risk Factor Analysis of ESBL-Producing Avian Pathogenic Escherichia coli Associated with Backyard Chickens in Pakistan. Antibiotics, 12(5), 934.
38. Sarker, M. S., Mannan, M. S., Ali, M. Y., Bayzid, M., Ahad, A., & Bupasha, Z. B. (2019). Antibiotic resistance of Escherichia coli isolated from broilers sold at live bird markets in Chattogram, Bangladesh. Journal of Advanced Veterinary and Animal Research, 6(3), 272.
39. Sattar, S., Hassan, M. M., Islam, S. K. M., Alam, M., Al Faruk, M. S., Chowdhury, S., & Saifuddin, A. K. M. (2014). Antibiotic residues in broiler and layer meat in Chittagong district of Bangladesh. Veterinary World, 7(9).
40. Saud, B., Paudel, G., Khichaju, S., Bajracharya, D., Dhungana, G., Awasthi, M. S., & Shrestha, V. (2019). Multidrug-resistant bacteria from raw meat of buffalo and chicken, Nepal. Veterinary medicine international, 2019.
41. Siddique, A., Azim, S., Ali, A., Andleeb, S., Ahsan, A., Imran, M., & Rahman, A. (2021). Antimicrobial resistance profiling of biofilm forming non typhoidal Salmonella enterica isolates from poultry and its associated food products from Pakistan. Antibiotics, 10(7), 785.
42. Sumanth, G., Jyoti, J., Anna, T., Anjana, S. L., & Ramanan, L. (2017). Scoping Report on Antimicrobial Resistance in India. Center for Disease Dynamics, Economics and Policy, Washington, DC.
43. Tahir, M. F., Afzal, F., & Athar, M. (2018). Prevalence and antimicrobial susceptibility patterns of Salmonella Enteritidis and Salmonella Typhimurium isolates from commercial poultry in Punjab, Pakistan. Iproceedings, 4(1), e10639.
44. Tang, B., Ma, Y., He, X., Zhou, Q., Chang, J., Qian, M., ... & Yang, H. (2021). Similar antimicrobial resistance of Escherichia coli strains isolated from retail chickens and poultry farms. Foodborne Pathogens and Disease, 18(7), 489-496.
45. Theobald, S., Etter, E. M. C., Gerber, D., & Abolnik, C. (2019). Antimicrobial resistance trends in Escherichia coli in South African poultry: 2009– 2015. Foodborne Pathogens and Disease, 16(9), 652-660.
46. Van den Bogaard, A. E., London, N., Driessen, C. A. G. G., & Stobberingh, E. E. (2001). Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of antimicrobial chemotherapy, 47(6), 763-771.
47. Vila, J., Sáez-López, E., Johnson, J. R., Römling, U., Dobrindt, U., Cantón, R., ... & Soto, S. M. (2016). Escherichia coli: an old friend with new tidings. FEMS microbiology reviews, 40(4), 437-463.
48. Waseem, H., Ali, J., Jamal, A., & Ali, M. I. (2019). Potential dissemination of antimicrobial resistance from small scale poultry slaughterhouses in Pakistan. Appl. Ecol. Environ. Res, 17, 3049-3063.
49. World Health Organization (WHO), Integrated Surveillance of Antimicrobial Resistance in Foodborne Bacteria, WHO, Geneva, Switzerland, 2017.
50. World Health Organization. (2014). Antimicrobial resistance: global report on surveillance. World Health Organization.
51. Zhang, Y., Lu, J., Yan, Y., Liu, J., & Wang, M. (2021). Antibiotic residues in cattle and sheep meat and human exposure assessment in southern Xinjiang, China. Food Science & Nutrition, 9(11), 6152-6161.

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