IN VIVO SAFETY ASSESSMENT OF CURCUMIN-ENCAPSULATED ZIF-8 AND ZIF-67 NANOCOMPOSITES: A TOXICITY STUDY
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Keywords
Curcumin, ZIF-8, ZIF-67, toxicolgy, In vivo
Abstract
Background: Curcumin, derived from the dried root rhizome of Curcuma longa, exhibits a spectrum of therapeutic effects, including antifungal, antibacterial, anti-inflammatory, antispasmodic, antioxidant, and antiarthritic properties. Despite these benefits, its low absorption limits its efficacy. Enhancing bioavailability through loading onto zeolitic metallic organic frameworks such as zeolitic imidazolate framework-8 (ZIF-8) and zeolitic imidazolate framework-67 (ZIF-67) presents a promising solution.
Objective: This study aimed to evaluate the acute toxicity of curcumin and its composites in male albino wistar rats.
Methods: Curcumin composites were synthesized using a solvothermal method. Male albino wistar rats were divided into seven groups (n=6/group) and administered curcumin, curcumin zeolitic imidazolate 8 (CZ8), and curcumin zeolitic imidazolate 67 (CZ67) at varying oral doses (30mg/kg, 60mg/kg) to experimental groups, and normal control group was treated with distilled water for 14 days. Throughout the study, body weight, behavior, and mortality were monitored. Blood and tissue samples were collected for analysis on the final day, with histopathological examination of vital organs (liver, kidney, spleen, brain, and heart) performed. Liver and kidney function tests, along with hematological parameters, were evaluated.
Results: No adverse effects or mortality were observed at the maximum dose of 60 mg/kg for either curcumin composite. Histopathological analysis revealed normal morphology in most organs at both doses. Slight alterations in liver tissue morphology were observed only at the 60mg/kg dose for CZ8 and CZ67. Liver and kidney function tests, and hematological parameters remained within normal ranges in all treatment groups.
Conclusion: Curcumin composites CZ8 and CZ67 demonstrated good biocompatibility and safety at doses up to 60mg/kg in this acute toxicity study.
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Hafiz Muhammad Asif
University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences,
The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Sehrish Rana Rajpoot
University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur - Pakistan
Khalil Ahmad
Department of Chemistry, University of Management and Technology (UMT), Sialkot Campus, Punjab - Pakistan
Muhammad Wasim Tasleem
Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur - Pakistan
Khalil Ahmad
University College of Conventional Medicine, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur - Pakistan