EVALUATION OF PRIMARY CYTOKINES AND LACTATE DEHYDROGENASE FROM NASOPHARYNGEAL SECRETIONS OF ACUTE OTITIS MEDIA

Main Article Content

Dr. Nilamadhaba Prusty
Dr. Dillip Kumar Pradhan
Dr. Biswaranjan Prusty
Dr. Siddharth Panditray

Keywords

Acute otitis Media, Nasopharyngeal secretions, Lactate dehydrogenase, cytokines

Abstract

Background: Acute otitis media (AOM) is a common side effect of a viral upper respiratory tract infection (URI). The severity of nasopharyngeal cellular injury during URI, as evaluated by lactate dehydrogenase (LDH) concentrations in nasopharyngeal secretions (NPSs), was hypothesized to be associated to AOM complication.


Methodology: LDH concentrations were measured in NPS samples (n = 110) obtained during the initial URI followed by the development of AOM. Interleukin IL-6, and tumor necrosis factor (TNF)- concentrations were also measured. LDH and Cytokines were measured by Immunoassay methods.  The LDH concentration minimal range was 0.9 and 1,000 mU/ml and the lowest cytokines detection limit of the assay was <10 pg/ml for IL-6 and alpha TNF.


Results: LDH concentrations independently predicted AOM complications. LDH concentrations were higher in URIs (P 0.05). There was a positive connection (P<0.001) between LDH concentrations and all cytokines.


Conclusion: LDH concentrations in NPS are related with an increased risk of AOM, implying that the severity of nasopharyngeal inflammatory injury during URI contributes to the development of AOM and that reducing inflammatory injury may lessen the risk of AOM.

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