IDENTIFICATION AND CHARACTERIZATION OF KLEBSIELLA PNEUMONIAE IN DIFFERENT CLINICAL SAMPLES AT PIMS HOSPITAL ISLAMABAD PAKISTAN.

Abstract

The main objective of the present study is the identification and characterization of klebsiella pneumoniae in different clinical samples in PIMS hospital Islamabad, Pakistan. There are 90 samples including 12 sputum samples, 21 tracheal swabs, 30 pus and 27 urine samples. Sample size: 90 subjects Duration: 6 months i.e., 01-07-2022 to 31-12-2022. The current study was conducted at PIMS hospital Islamabad. Gram staining, microscopy, sputum, tracheal swab, pus, and urine cultures, biochemical tests, and antibiotic susceptibility testing were performed for all samples. Statistical analysis was performed by using SPSS Software. Result of the current study show that among the 90 clinical samples, 50 (55.6%) were positive for K. pneumonia and 10 cases (11.1%) with other bacteria. 30 (33.3%) cases showed no growth. Colistin, meropenem, amikacin, and imipenem (8.3%) were sensitive to sputum samples. Colistin 38.1%, ceftazidime 4.7%, meropenem 4.7%, amikacin 9.5%, sulfamethoxazole 4.7%, gentamycin 9.5%, and imipenem 28.5% were sensitive in tracheal swab samples. Conclusion: The highest sensitivity was observed incefoperazone, cefotaxime, and ceftriaxone (53.3%) isolates in pus samples.