PHARMACOLOGICAL EVALUATION OF THE ETHANOLIC EXTRACT OF MANGIFERA INDICA LEAVES AGAINST ETHYLENE GLYCOL-INDUCED UROLITHIASIS IN WISTAR ALBINO RATS.

Main Article Content

Shaik Mohemmed Zeeshan Ali
Dr. MD Sultan Ali Basha
Preethi Kuruva
Gotla Anand
D. Riyazunnisa
Shaik Mahaboob Bee

Keywords

Urolithiasis, Ethanolic Extract, Mangifera indica, Anti-lithiatic, Hyperoxaluria, Calcium Oxalate, Natural Remedy

Abstract

Urolithiasis, the formation of kidney stones, represents a prevalent and distressing urological disorder. In this study, the potential antiurolithiatic activity of ethanolic extract of Mangifera indica leaves (EEMI) was investigated using in vitro assays and an ethylene glycol-induced urolithiatic rat model. EEMI exhibited significant inhibitory effects on calcium oxalate crystal nucleation and aggregation, crucial steps in stone formation. Moreover, EEMI demonstrated a low toxicity profile in acute toxicity assessments, affirming its safety. In an ethylene glycol-induced rat model, EEMI effectively restored serum and urinary parameters, indicating its ability to preserve kidney function and mitigate hyperoxaluria. These findings highlight the promise of EEMI as a potential natural remedy for kidney stone prevention and management, warranting further research and clinical studies.

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References

1. Scales Jr CD, Smith AC, Hanley JM, Saigal CS, Urologic Diseases in America Project. Prevalence of kidney stones in the United States. European urology. 2012 Jul 1;62(1):160-5.
2. Sohgaura A, Bigoniya P. A review on epidemiology and etiology of renal stone. Am J Drug Discov Dev. 2017 Mar 15;7(2):54-62.
3. Smyslova OA, Markaryan AA, Evdokimova OV, Glazkova IU, Yaroshenko MA. Characteristics of the new comprehensive herbal medicine for the treatment and prevention of urolithiasis. Biology and Medicine. 2015 Apr 1;7(2):1.
4. Rathod NR, Biswas D, Chitme HR, Ratna S, Muchandi IS, Chandra R. Anti-urolithiatic effects of Punica granatum in male rats. Journal of ethnopharmacology. 2012 Mar 27;140(2):234-8.
5. Nabi G, Downey P, Keeley FX, Watson GM, McClinton S. Extra‐corporeal shock wave lithotripsy (ESWL) versus ureteroscopic management for ureteric calculi. Cochrane database of systematic reviews. 2007(1).
6. Aeckart KS, Schroder FH. Effect of extra corporeal shock wave lithotripsy (ESWL) on renal tissue. Urol Res. 1989;17(1):3-7.
7. Kieley S, Dwivedi R, Monga M. Ayurvedic medicine and renal calculi. Journal of endourology. 2008 Aug 1;22(8):1613-6.
8. Shah KA, Patel MB, Patel RJ, Parmar PK. Mangifera indica (mango). Pharmacognosy reviews. 2010 Jan;4(7):42.
9. Masibo M, He Q. Mango bioactive compounds and related nutraceutical properties—a review. Food Reviews International. 2009 Sep 29;25(4):346-70.
10. Tamayo D, Mari E, González S, Guevara M, Garrido G, Delgado R, Marchioli R, Núñez AJ. Vimang as natural antioxidant supplementation in patients with malignant tumors. Minerva Med. 2001;92(suppl 1-3).
11. Ediriweera MK, Tennekoon KH, Samarakoon SR. A review on ethnopharmacological applications, pharmacological activities, and bioactive compounds of Mangifera indica (Mango). Evidence-Based Complementary and Alternative Medicine. 2017 Dec 31;2017.
12. Umamahesh K, Ramesh B, Kumar BV, Reddy OV. In vitro anti-oxidant, anti-microbial and anti-inflammatory activities of five Indian cultivars of mango (Mangifera indica L.) fruit peel extracts. Journal of Herbmed Pharmacology. 2019 May 9;8(3):238-47.
13. Bai X, Lai T, Zhou T, Li Y, Li X, Zhang H. In vitro antioxidant activities of phenols and oleanolic acid from mango peel and their cytotoxic effect on A549 cell line. Molecules. 2018 Jun 8;23(6):1395.
14. Abbasi AM, Liu F, Guo X, Fu X, Li T, Liu RH. Phytochemical composition, cellular antioxidant capacity and antiproliferative activity in mango (Mangifera indica L.) pulp and peel. International Journal of Food Science & Technology. 2017 Mar;52(3):817-26.
15. Abdullah AS, Mohammed AS, Rasedee A, Mirghani ME. Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells. International journal of molecular sciences. 2015 Feb 5;16(2):3528-36.
16. Gondi M, Basha SA, Bhaskar JJ, Salimath PV, Prasada Rao UJ. Anti‐diabetic effect of dietary mango (Mangifera indica L.) peel in streptozotocin‐induced diabetic rats. Journal of the Science of Food and Agriculture. 2015 Mar 30;95(5):991-9.
17. Sekar V, Chakraborty S, Mani S, Sali VK, Vasanthi HR. Mangiferin from Mangifera indica fruits reduces post-prandial glucose level by inhibiting α-glucosidase and α-amylase activity. South African journal of botany. 2019 Jan 1;120:129-34.
18. Rajesh MS, Rajshekar J. Assesment of antidiabetic activity of Mangifera Indica seed kernel extracts in streptozotocin induced diabetic rats. Journal of Natural Remedies. 2014:33-40.
19. Mirza B, Croley CR, Ahmad M, Pumarol J, Das N, Sethi G, Bishayee A. Mango (Mangifera indica L.): a magnificent plant with cancer preventive and anticancer therapeutic potential. Critical Reviews in Food Science and Nutrition. 2021 Jul 20;61(13):2125-51.
20. Du S, Liu H, Lei T, Xie X, Wang H, He X, Tong R, Wang Y. Mangiferin: An effective therapeutic agent against several disorders. Molecular medicine reports. 2018 Dec 1;18(6):4775-86.
21. In OECD. Acute oral toxicity-Acute oral toxic class method. Guideline 423. adopted 23/06/1996) Eleventh Addendum to the OECD guidelines for the testing of chemicals, Organization for Economic Co-operation and Development: Paris, 2001, accessed on 21st January, 2018, at http://www. research. murdoch. edu. Au /ethics /arec /oraltoxicity.
22. Sathish R, Natarajan K, Nikhad MM. Effect of Hygrophila spinosa T. anders on ethylene glycol induced urolithiasis in rats. Asian Journal of Pharmaceutical and Clinical Research. 2010;3(4):61-3.