Comparison of Chrysopogon zizanioides mouthwash with Chlorhexidine mouthwash in Chronic Periodontitis Patients-An Clinical trial

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Jaiganesh Ramamurthy


Periodontitis, Chrysopogon zizanioides Chlorhexidine, Mouthwash


Introduction: Chrysopogon zizanioides (Vetiver grass) is the source of a valuable essential oil, for which purpose it is often grown commercially. Amongst its many other uses, it provides material for thatch, has many medicinal applications and is cultivated to protect the soil from erosion. People take vetiver for nerve and circulation problems and for stomach pain. Vetiver is sometimes inhaled as aromatherapy for nervousness,insomnia, and joint and muscle pain. Vetiver oil has been used as anti-inflammatory, antiseptics, antioxidant and antibacterial activities.
Materials and methods: Vetiver leaves have been extracted from and leaves are dried for 10 days then the extracted leaves are used for mouthwash preparation. The aqueous extract of prepared leaves was used for mouthwash preparation. Mouthwash preparation was done by the aqueous extract of chrysopogon zizanioides .20 patients with chronic periodontitis were selected for the study. In which 10 patients with the test group had Chrysopogon zizanioides mouthwash and 10 patients with the control group were given with chlorhexidine mouthwash. Each mouthwash was asked to use twice daily. Clinical parameters were assessed Probing depth, clinical attachment level and Bleeding index at baseline till 14 days.
Results: Results of the study showed that chrysopogon zizanioides and chlorhexidine are equally effective in reducing plaque, gingival, and bleeding indices at 14-day intervals. However, no significant reductions in PD, CAL and BI in the 14-day interval in group 1 (Chrysopogon zizanioides) and group 2(Chlorhexidine mouthwash) when compared with chlorhexidine were evident.
Conclusion: The results in the present study indicate that chrysopogon zizanioides may prove to be as effective as chlorhexidine mouthwash in its ability in reducing all the three indices by reducing probing depth, clinical attachment level and bleeding index.

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1. Antiochia R, Campanella L, Ghezzi P, Movassaghi K. The use of vetiver for remediation of heavy metal soil contamination. Analytical and Bioanalytical Chemistry. 2007;388(4):947-956. doi:10.1007/s00216-007-1268-1
2. Website. Antiochia R, Campanella L, Ghezzi P, Movassaghi K. The use of vetiver for remediation of heavy metal soil contamination [Internet]. Vol. 388, Analytical and Bioanalytical Chemistry. 2007. p. 947–56. Available from:
3. Chen Y, Shen Z, Li X. The use of vetiver grass (Vetiveria zizanioides) in the phytoremediation of soils contaminated with heavy metals. Applied Geochemistry. 2004;19(10):1553-1565. doi:10.1016/j.apgeochem.2004.02.003
4. Phytoremediation of Soils Contaminated by Heavy Metals, Metalloids, and Radioactive Materials Using Vetiver Grass, Chrysopogon zizanioides. Phytotechnologies. Published online 2012:280-307. doi:10.1201/b12954-17
5. Joshua MT, Department of Medical Laboratory Science, Rivers State University Port Harcourt. Evaluation of the effects of Aqueous and Ethanolic Extracts of Morus mesozygia Linn. Stapf., Leaves on Lipid Profile and Renal Indices of Streptozotocin- Induced Diabetic Rats. Journal of Medical Science And clinical Research. 2019;7(9). doi:10.18535/jmscr/v7i9.77
6. Nix KE, Henderson G, Zhu BCR, Laine RA. Evaluation of Vetiver Grass Root Growth, Oil Distribution, and Repellency Against Formosan Subterranean Termites. HortScience. 2006;41(1):167-171. doi:10.21273/hortsci.41.1.167
7. Maistrello L, Henderson G, Laine RA. Efficacy of Vetiver Oil and Nootkatone as Soil Barriers Against Formosan Subterranean Termite (Isoptera: Rhinotermitidae). Journal of Economic Entomology. 2001;94(6):1532-1537. doi:10.1603/0022-0493-94.6.1532
8. Craig WJ. Health-promoting properties of common herbs. The American Journal of Clinical Nutrition. 1999;70(3):491s - 499s. doi:10.1093/ajcn/70.3.491s
9. Egamberdieva D, Shrivastava S, Varma A. Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants. Springer; 2015.
10. Lang NP, Lindhe J. Clinical Periodontology and Implant Dentistry, 2 Volume Set. John Wiley & Sons; 2015.
11. Vyas SP, Sihorkar V, Mishra V. Controlled and targeted drug delivery strategies towards intraperiodontal pocket diseases. Journal of Clinical Pharmacy and Therapeutics. 2000;25(1):21-42. doi:10.1046/j.1365-2710.2000.00261.x
12. Van Leeuwen MPC, Slot DE, Van der Weijden GA. Essential Oils Compared to Chlorhexidine With Respect to Plaque and Parameters of Gingival Inflammation: A Systematic Review. Journal of Periodontology. 2011;82(2):174-194. doi:10.1902/jop.2010.100266
13. Rølla G, Løe H, Rindom Schiøtt C. Retention of chlorhexidine in the human oral cavity. Archives of Oral Biology. 1971;16(9):1109-IN33. doi:10.1016/0003-9969(71)90215-9
14. Flötra L, Gjermo P, Rölla G, Waerhaug J. Side effects of chlorhexidine mouth washes. European Journal of Oral Sciences. 1971;79(2):119-125. doi:10.1111/j.1600-0722.1971.tb02001.x
15. Loe H, Rindom Schiott C. The effect of mouthrinses and topical application of chlorhexidine on the development of dental plaque and gingivitis in man. Journal of Periodontal Research. 1970;5(2):79-83. doi:10.1111/j.1600-0765.1970.tb00696.x
16. Afennich F, Slot DE, Hossainian N, Van der Weijden GA. The effect of hexetidine mouthwash on the prevention of plaque and gingival inflammation: a systematic review. Int J Dent Hyg. 2011;9(3):182-190.
17. James P, Worthington HV, Parnell C, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health. Cochrane Database Syst Rev. 2017;3:CD008676. 18. Eick S, Goltz S, Nietzsche S, Jentsch H, Pfister W. Efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms. Quintessence Int. 2011;42(8):687-700.
19. Fedorowicz Z, Aljufairi H, Nasser M, Outhouse TL, Pedrazzi V. Mouthrinses for the treatment of halitosis. Cochrane Database Syst Rev. 2008;(4):CD006701.
20. Zakariya U, Umar U, Dambazau S, Sulaiman A. Comparative Hepatotoxic Effects of Aqueous and Phenolic Extracts of Avocado (Persea americana) Seed in Wistar Albino Rats. International Journal of Biochemistry Research & Review. 2016;10(4):1-6. doi:10.9734/ijbcrr/2016/23196
21. Saikia D, Parveen S, Gupta VK, Luqman S. Anti-tuberculosis activity of Indian grass KHUS (Vetiveria zizanioides L. Nash). Complementary Therapies in Medicine. 2012;20(6):434-436. doi:10.1016/j.ctim.2012.07.010
22. Gupta R, Sharma KK, Afzal M, et al. Anticonvulsant activity of ethanol extracts ofVetiveria zizanioidesroots in experimental mice. Pharmaceutical Biology. 2013;51(12):1521-1524. doi:10.3109/13880209.2013.799710
23. Sinha S, Jothiramajayam M, Ghosh M, Jana A, Chatterji U, Mukherjee A. Vetiver oil (Java) attenuates cisplatin-induced oxidative stress,
nephrotoxicity and myelosuppression in Swiss albino mice. Food and Chemical Toxicology. 2015;81:120-128. doi:10.1016/j.fct.2015.04.018
24. Luqman S, Srivastava S, Darokar MP, Khanuja SPS. Detection of Antibacterial Activity in Spent Roots of Two Genotypes of Aromatic GrassVetiveria zizanioides. Pharmaceutical Biology. 2005;43(8):732-736. doi:10.1080/13880200500387471
25. Evaluation of Cytotoxic Activity of Chrysopogon Zizanioides Mouthwash - An In Vitro Study. International Journal of Pharmaceutical Research. 2020;12(sp1). doi:10.31838/ijpr/2020.sp1.431
26. Afkhami F, Akbari S, Chiniforush N. Entrococcus faecalis Elimination in Root Canals Using Silver Nanoparticles, Photodynamic Therapy, Diode Laser, or Laser-activated Nanoparticles: An In Vitro Study. J Endod. 2017;43(2):279-282.
27. Orstavik D. Essential Endodontology: Prevention and Treatment of Apical Periodontitis. John Wiley & Sons; 2020.
28. Sofiani E, Wahyuningrum H. Differential effectiveness of calcium hydroxide with 2% chlorhexidine digluconate and 25% propolis as a root canal medicament against Enterococcus faecalis (In vitro). Scientific Dental Journal. 2021;5(1):37. doi:10.4103/sdj.sdj_45_20