HISTOPATHOLOGICAL AND BIOCHEMICAL STUDY OF SILYMARIN AND VITAMIN C VERSUS OMEPRAZOLE IN THE TREATMENT OF GASTRIC ULCER INDUCED BY INDOMETHACIN IN ADULT MALE ALBINO RATS.
Main Article Content
Keywords
Silymarin, vitamin C indomethacin-induced stomach ulcers, male albino rats, Gastric ulcers.
Abstract
Objective: This study aims at investigating the role of each of Silymarin and Vitamin C in preventing gastric Ulcer induced by indomethacin in comparable with omeprazole.
Method:
A study was conducted on 40 adult male albino rats. The rats were divided into two groups: a control group, which received distilled water orally, and an experimental group, which received indomethacin intraperitoneally for two consecutive days to induce stomach ulcers. After inducing the gastric ulcer, the rats were divided into three groups: Silymarin, Vitamin C, and Omeprazole. Blood samples were collected for biochemical evaluation, and the rats were euthanized by cervical dislocation three hours after the duodenum was ligated. The gastric juice was examined for volume, titratable acidity, and total acid output. Histological examination of the stomach was performed after the opening was checked for ulcers.
Results:
The study compared three groups before and after treatment with indomethacin injection, followed by treatment with Silymarin and vitamin C. The results showed that Silymarin and vitamin C achieved the best results, followed by omeprazole. The average ulcer area, number of ulcers, and stomach size varied among the groups. After treatment, the ulcer area decreased by 63% in the first group treated with Silymarin, while the number of ulcers decreased by 27% in the second group treated with Vitamin C. The size of the stomach increased by 27.5% in the first group, 17% in the second group, and 67% in the third group.
Conclusion: This study illustrates the efficiency of Silymarin and Vitamin C in alleviating indomethacin-induced stomach ulcers in albino rats, both showing significant effectiveness relative to omeprazole. Silymarin exhibited a marked reduction in ulcer area, whereas Vitamin C indicated a notable decrease in ulcer quantity. Silymarin and Vitamin C produced a lesser increase in stomach size than omeprazole, indicating a potentially superior management of gastric distension.
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Hassan saad Hassan Elwakeel
Department of Anatomy and Embryology, Faculty of Medicine , Al-Azhar University, Damitta, Egypt.
Manar A. Elkholy
Department of Pharmacology, Faculty of Medicine, Benha University, Egypt.
Hammad Yahia Abdoh
Department of Anatomy and Embryology, Faculty of Medicine , Al-Azhar University, Damitta, Egypt.
Alaa AboSeif
Department of Pharmacology, Faculty of Medicine, Al Azhar University, Cairo, Egypt.
Faten I. Mohammed
Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University , Cairo, Egypt.
Ahmed Abdrabo Elshenawy Elsisi
Department of Pharmacology , Faculty of Medicine , Al-Azher University, Asyut, Egypt.
Shaimaa fawzy Abdellatif Esmaeil
Department of physiology, Faculty of Medicine for Girls, Al-Azhar University, Asyut, Egypt.
Noha O. Shawky
Department of Medical Physiology, Faculty of Medicine, Helwan University, Egypt.