In vitro Anti-inflammatory activity of Silymarin/Hydroxyapatite/Chitosan Nanocomposites and its cytotoxic effect using Brine shrimp lethality assay Nanocomposite for biomedical applications

Main Article Content

Rajeshkumar S
Aravind Kumar Subramanian
Ramachandra Prabhakar
Raj Vikram N
saravanan dinesh SP

Keywords

Nanocomposites, Anti-inflammatory, Cytotoxic, Chitosan, silymarin

Abstract

The bioactive compound silymarin is one of the most prominent drug used in liver diseases. The chitosan and hydroxyapatite is major materials used in many biomedical applications such as drug delivery, osteointegration etc. The nanoparticles and nanocomposites are advanced materials with many biomedical applications in diagnosis and therapeutics. In this present investigation we have used Silymarin a bioactive compound, hydroxyapatite and chitosan a polymeric material and based different combinations. The prepared individual materials and nanocomposites were used for the anti-inflammatory activity and brine shrimp lethality assay. The results are clearly shows that the nanomcposites are very good anti-inflammatory agent with lower toxicity. 

Abstract 61 | PDF Downloads 59 XML Downloads 13 HTML Downloads 20

References

1. Oyedapo OA, Adewunmi CO, Iwalewa EO, Makanju VO. Analgesic, antioxidant and anti-inflammatory related activities of 21-hydroxy-2, 41-dimethoxychalcone and 4-hydroxychalcone in mice. J Biol Sci 2008;8(1):131-6.
2. Dinarello CA. Anti-inflammatory agents: present and future. Cell 2010;140(6):935-50.
3. Anilkumar M. Ethnomedicinal plants as anti-inflammatory and analgesic agents. In: Chattopadhyay D, editor. Ethnomedicine: A source of complementary therapeutics 2010. p. 267-93.
4. Kiringe JW (2006) A Survey of Traditional Health Remedies Used by the Maasai of Southern Kaijiado District. Kenya Ethnobotany Research & Applications 4: 061-073.
5. Soetan KO, Aiyelaagbe OO (2009) The need for bioactivity-safety evaluation and conservation of medicinal plants. A review Journal of Medicinal Plants Research 3: 324-328.
6. Recio MC, Andujar I, Rios JL (2012) Anti-inflammatory agents from plants: progress and potential. Curr Med Chem 19: 2088-2103.
7. Elisabetsky E, Amador TA, Albuquerque RR, Nunes DS, Carvalho Ado C (1995) Analgesic activity of Psychotria colorata (Willd. ex R. & S.) Muell. Arg. alkaloids. J Ethnopharmacol 48: 77-83.
8. Yodsaoue O, Karalai C, Ponglimanont C, Tewtrakul S, Chantrapromma S (2010) Potential anti-inflammatory diterpenoids from the roots of Caesalpinia mimosoides Lamk. Phytochemistry 71: 1756-1764.
9. Anthony KP, Saleh MA. Free radical scavenging and antioxidant activities of silymarin components. Antioxidants. 2013;2(4):398–407.
10. Freedman ND, Curto TM, Morishima C, Seeff LB, Goodman ZD, Wright EC, et al. Silymarin use and liver disease progression in the Hepatitis C Antiviral Long-Term Treatment against Cirrhosis trial. Aliment Pharmacol Ther. 2011;33(1):127–37.
11. Calani L, Brighenti F, Bruni R, Del Rio D. Absorption and metabolism of milk thistle flavanolignans in humans. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2012;20(1):40–6.
12. Rajeshkumar, S., Sherif, M.H., Malarkodi, C., Ponnanikajamideen, M., Arasu, M.V., Al-Dhabi, N.A. and Roopan, S.M., 2020. Cytotoxicity behaviour of response surface model optimized gold nanoparticles by utilizing fucoidan extracted from padina tetrastromatica. Journal of Molecular Structure, p.129440.
13. Aravind Kumar, Rajeshkumar S et al. (2020) Antimicrobial activity of silymarin mediated zinc oxide and hydroxy apatite nanoparticles against oral pathogens Bioinformation 16(11): 863-868.
14. Ganapathy D, Rajeshkumar S, Lakshmi T (2020) Nanobiotechnology in combating CoVid-19 Bioinformation 16(11): 828-830
15. Shuang Wu, Shanmugam Rajeshkumar, Malini Madasamy & Vanaja Mahendran (2020) Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens, Artificial Cells, Nanomedicine, and Biotechnology, 48:1, 1153-1158, DOI: 10.1080/21691401.2020.1817053.
16. Devi, B. V., Rajasekar, A., & Rajeshkumar, S. (2020). Antiinflammatory Activity Of Zinc Oxide Nanoparticles Synthesised Using Grape Seed Extract: An In Vitro Study. Plant Cell Biotechnology And Molecular Biology, 21(33-34), 6-16.
17. Francis T, Rajeshkumar S, Roy A, Lakshmi T. Anti-inflammatory and Cytotoxic Effect of Arrow Root Mediated Selenium Nanoparticles. Pharmacogn J. 2020;12(6):1363-7. DOI : 10.5530/pj.2020.12.188.