ACUTE FEBRILE FEVER WITH DENGUE VIRUS INFECTION AT DIAGNOSTIC AND RESEARCH LABORATORY PUMHSW, SHAHEEDBENAZIRABAD (SBA)
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Keywords
Acute febrile fever, dengue fever, Mac-ELISA
Abstract
Objective: To determine frequency of dengue virus infection among acute febrile fever patients. To assess an association of hematological parameters with dengue fever.
Study Design: A cross-sectional study
Place and duration: Diagnostic and Research (DR) Laboratory of Pathology Department PUMHSW(SBA) from January 2022 to June 2022.
Methodology: This study was conducted on 240 blood samples from acute febrile patients. Serum was used for serology while EDTA test tubes were used for hematological parameters. Samples were tested for the presence of NS1 antigen and dengue IgM antibody. Dengue NS1 antigen was detected in laboratory by using rapid device test (DFI diagnostics) the immune-chromatographic technique, while serological testing IgM antibody was detected by ELISA (Mac-ELISA). Panbio™ Dengue IgM Capture ELISA kits were used. Hematological findings were derived from complete blood count and recorded.
Results: A sum of 240 cases presented with classical characteristics of Dengue fever. Out of which majority of the cases were in their third decade of life expectancy with median age of 30.5 years. A total of 240 patients were tested. Out of these 36(15%) were found to be positive for dengue virus and remaining 204 (85%) appeared to be negative for dengue. Among Dengue negatives, 18(8.82%) and 10(4.90%) were positive (+ve). for malarial parasite (M.P) and typhoid fever(typhidot) consecutively. Strikingly 176 (86.28%) patients remained undiagnosed.
Conclusion: WHO states that RDTs are less reliable and sensitive. So it is need of hour to apply an advanced and sophisticated approach for confirmation. Mac-ELISA confirms and testifies rapid and diagnostic tests.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Amir Bux Detho
Associate Professor, Department of Pathology PUMHS(W) Nawabshah (SBA), Sindh Pakistan
Sikandar Ali Siyal
Associate Professor, Department of Pathology PUMHS(W) Nawabshah (SBA), Sindh Pakistan.
Arooj Ali
Lecturer & Postgraduate Student, Department of Pathology PUMHS(W) Nawabshah (SBA), Sindh Pakistan.
Abid Hussain Chang
Professor, Department of Pathology, Liaquat University of Medical & Health Sciences, Jamshoro Sindh Pakistan.
Hina Sabeen
Lecturer, Department of Pathology PUMHS(W) Nawabshah (SBA), Sindh Pakistan.
Sandhiya Kumari
Postgraduate Student, Department of Pathology PUMHS(W) Nawabshah (SBA), Sindh Pakistan.