ANTI-ERYPTOTIC POTENTIAL OF SELENIUM AGAINST GENTAMICIN-INDUCED ERYPTOSIS
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Abstract
Gentamicin, an aminoglycoside, is used to treat serious infections such as blood infections (septicemia) and brain and spinal cord infections. Recent years have shown an increase in antibiotic use, which can be associated with side effects like eryptosis, a process mainly induced by oxidative stress, ceramide production, and energy depletion, which can lead to anemia. Eryptosis, quite similar to apoptosis, is defined by cell shrinkage, membrane blebbing, and membrane phospholipid scrambling with phosphatidylserine exposure at the cell surface. The core purpose of this research was to examine the eryptotic effect of gentamicin through the oxidative stress pathway and study the anti-eryptotic potential of selenium against gentamicin-induced eryptosis. In this study, erythrocytes were treated with therapeutical doses (9-18μM) of gentamicin and selenium for 48 hours according to experimental requirements. To assess the cytotoxicity of gentamicin hemolysis% was estimated, the oxidative potential of gentamicin and the anti-oxidative potential of selenium was confirmed by investigating the levels of glutathione peroxidase, superoxide dismutase and catalase, to confirm their eryptotic role mean cell volume(MCV) was measured, role of Ca+2 in eryptosis was confirmed by using a calcium-channel blocker, amlodipine. Results illustrate that an increase in gentamicin concentration directly affects hemolysis%, gentamicin treated cells showed low levels of enzyme activity while gentamicin+ selenium-treated cells showed better activity of antioxidants, less than normal MCV was observed in gentamicin-treated cells which is a direct indication that cell is going through eryptosis, calcium channel blocker showed the involvement of Ca2+ in eryptosis.
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Muhammad Ahmad Majeed
Department of Biochemistry, University of Agriculture Faisalabad, Pakistan
Maham Abdul Bari Khan
Department of Biochemistry, University of Agriculture Faisalabad, Pakistan
Kinza Khalid
Department of Biochemistry, University of Agriculture Faisalabad, Pakistan
Muhammad Hasnain
Institute of Molecular Aggregation, School of Science, Tianjin University, Tianjin 300072, China
Abdul Qayoom
Academy of Medical Engineering and Translational Medicine, Medical College, Tianjin University, Tianjin 300072, China
Aneeza Kokab
Institute of Chemistry, University of Okara, Renala Khurd, Pakistan
Hafiz Muhammad Aslam
Department of Biochemistry, University of Agriculture Faisalabad, Pakistan
Kashif Jilani
Department of Biochemistry, University of Agriculture Faisalabad, Pakistan