IMPACT OF DIETARY SUPRA NUTRITIONAL SELENIUM SUPPLEMENTATION ON FAT METABOLISM IN THE SKELETAL MUSCLES OF GOATS
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Keywords
Selenium, Supplementation, Fat metabolism, Skeletal muscles, Goat
Abstract
This study aimed to evaluate the effects of dietary supranutritional selenium (Se) supplementation on gene expression related to fat metabolism in the skeletal muscles of male goats. Sixteen male goats, aged 3-4 months and weighing 10-13 kg, were randomly divided into two groups (n=8 each) and housed individually at the Livestock Experimental Station, Sindh Agriculture University, Tandojam. The goats underwent a 2-week adaptation period, during which they were offered a diet containing concentrate and forage. Afterward, they were fed a basal diet with two different Se levels: Group A (control) received 0.3 mg/kg diet Se, while Group B (treatment) received 0.65 mg/kg diet Se, with selenium yeast (Sel-Plex®, Alltech®, USA) as the organic Se source. Over the 10-week experimental period, mRNA expression levels of genes involved in fat metabolism, including peroxisome proliferator-activated receptor alpha (PPARα), lipoprotein lipase (LPL), carnitine palmitoyltransferase 1 (CPT-1), cytosolic phospholipase A2 (cPLA2), and heart-type fatty acid-binding protein (H-FABP), were analyzed via quantitative real-time PCR. Results indicated a significant increase (P < 0.05) in the expression of these genes in Group B compared to Group A. In conclusion, this study demonstrates that dietary supranutritional selenium supplementation significantly enhances the expression of key genes involved in fat metabolism, such as PPARα, LPL, CPT-1, cPLA2, and H-FABP, in the skeletal muscles of goats. The upregulation of these genes suggests improved lipid utilization and oxidative capacity, which may contribute to better energy production and meat quality.
References
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Muhammad Anees Memon
Department of Veterinary Physiology and Biochemistry, Sindh Agriculture University Tandojam, Pakistan
Moolchand Malhi
Department of Veterinary Physiology and Biochemistry, Sindh Agriculture University Tandojam, Pakistan
Allah Bux Kachiwal
Department of Veterinary Physiology and Biochemistry, Sindh Agriculture University Tandojam, Pakistan
Ghulam Shabir Barham
Department of Animal Product Technology, Sindh Agriculture University Tandojam, Pakistan
Mohammad Farooque Hassan
Department of Veterinary Pathology, SBBUVAS Sakrand, Pakistan
Ghulam Murtaza Lochi
Department of Veterinary Anatomy and histology, Sindh Agriculture University Tandojam, Pakistan
Tarique Ahmed Khokhar
Department of Animal Nutrition, SBBUVAS Sakrand, Pakistan
Syed Abdul Hadi
Department of Veterinary Anatomy and histology, Sindh Agriculture University Tandojam, Pakistan
Abdul Kabir
Department of Veterinary Microbiology and Biochemistry, Sindh Agriculture University Tandojam, Pakistan