A COMPARATIVE STUDY OF IMMUNOHISTOCHEMICAL MARKERS IN THE DETECTION OF EARLY MYOCARDIAL INFARCTION

Dr Ritu Saxena

doi:10.53555/jptcp.v26i3.6516

Dr Ritu Saxena

Keywords

atherosclerosis, pathology, risk factors, markers

Abstract

immunohistochemical techniques have proven effective in forensic pathology use as they can be applied to tissue samples fixed in formalin and embedded in paraffin. In this work, we selected a panel of antibodies, desmin, troponin T (cTnT), and myoglobin (MB), for the detection of early

myocardial infarction in human autopsy hearts with suspected sudden cardiac death to compare the sensitivity and the reliability of these markers and to study the potential effect of autolysis on their sensitivity. Histologic sections from the myocardium from 20 autopsy cases were studied by immunohistochemistry with the three different antibodies, using a standard avidin–biotin–peroxidise system. Desmin, cTnT, and MB depletion occurred very

early after ischemic injury and preceded the histologic

Abstract 12 | Pdf Downloads 2

References

1. Amin HAA, El-Hennawy AMY, Nakhla GAA, Tabak SA, Hassan HH (2011).Immunohistochemistry in The Detection of Early Myocardial Infarction (A Postmortem Study). Egypt J Forensic Sci 1:5–12.
2. Baroldi G (2001). Myocardial cell death, including ischemic heart disease and itscomplication. In: Silver MD, Gotlieb AI, Schoen FJ, editors. Cardio-vascular pathology. 3rd ed. Philadelphia: Churchill Livingstone. pp. 198–225.
3. Bouchardy B, Majno G (1974). Histopathology of early myocardial infarcts. A new approach. Am J Pathol 74:301–330.
4. Brinkmann B, Sepulchre MA, Fechner G (1993). The application of selected histochemical and immunohistochemical markers and procedures to the diagnosis of early myocardial damage. Int J Legal Med 106:135–141.
5. Campobasso CP, Dell’Erba AS, Addante A, Zotti F, Marzullo A, Colonna MF (2008). Sudden cardiac death and myocardial ischemia indicators: a comparative study of four immunohistochemical markers. Am J Forensic Med Pathol 29:154–161.
6. Doran JP, Howie AJ, Townend JN, Bonser RS (1996). Detection of myocardial infarction by immunohistological staining for C9 on formalin fixed, paraffin wax embedded sections. J Clin Pathol 49:34–37.
7. Edwards WD (2003). Cardiovascular system in handbook of autopsy practice, 3rd ed. Totowa, NJ: Human Press. p. 21.
8. Gornall DA, Roth SN (1996). Serial myoglobin quantitation in the early assessment of myocardial damage: a clinical study. Clin Biochem 29: 379–384.
9. Hansen SH, Rossen K (1999). Evaluation of cardiac troponin I immunoreaction in autopsy hearts: a possible marker of early myocardial infarction. Forensic Sci Int 99:189–196.
10. Hein S, Scheffold T, Schaper J (1995). Ischemia induces early changes to cytoskeletal and contractile proteins in diseased human myocardium. J Thorac Cardiovasc Surg 110:89–98.
11. Ishikawa Y, Akasaka Y, Ishii T, Yoda-Murakami M, Choi-Miura NH, Tomita M, et al (2003). Changes in the distribution pattern of gelatin-binding protein of 28 kDa (adiponectin) in myocardial remodelling after ischaemic injury. Histo- pathology 42:43–52.
12. Jie O, Miguel G, Lapaix FD, Pincus MR, Rosemary W (2010). Original article utility of desmin and a Masson’s trichrome method to detect early acute myocardial infarction in autopsy tissues. Int J Clin Exp Pathol 3:98–105.
13. Lodge-Patch I (1951). The ageing of cardiac infarcts, and its influence on cardiac rupture. Br Heart J 13:37–42.
14. Milner DJ, Taffet GE, Wang X, Pham T, Tamura T, Hartley C, et al (1999). The absence of desmin leads to cardiomyocyte hypertrophy and cardiac dilation with compromised systolic function. J Mol Cell Cardiol 31:2063–2076. Ortmann C, Pfeiffer H, Brinkmann B (2000). A comparative study on the immunohistochemical detection of early myocardial damage. Int J Legal Med 113:215–220.
15. Perez-Carceles MD, Noguera J, Luna A, Osuna E (2005). Biochemical analysis and immuno-histochemical determination of cardiac troponin for the postmortem diagnosis of myocardial damage. Histol Histopathol 20: 475–481.
16. Sarko J, Charles V, Pollack MA Jr (2002). Cardiac troponins I and T in patients with suspected acute coronary syndrome. J Emerg Med 23:57–65. Shabaiek AA, Ismael NEHS, Elsheikh SA, Amin HAA (2014). Role of cardiac myocytes heart fatty acid binding protein depletion (h-fabp) in early myocardial infarction in human heart [MSc thesis]. Egypt: Kasr El-Aini Hospital.
17. Thiene G, Basso C, Corrado D (2001). Cardiovascular causes of sudden death.
18. In: Silver MD, Gotlieb AI, Schoen FJ, editors. Cardiovascular pathology.3rd ed. Philadelphia: Churchill Livingstone. pp. 326–374.
19. Yoshida K (2009). Pursuing enigmas on ischemic heart disease and sudden cardiac death., Leg Med (Tokyo) 11:51–58

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Published

Mar 31, 2019

DOI https://doi.org/10.53555/jptcp.v26i3.6516

How to Cite

Dr Ritu Saxena. (2019). A COMPARATIVE STUDY OF IMMUNOHISTOCHEMICAL MARKERS IN THE DETECTION OF EARLY MYOCARDIAL INFARCTION. Journal of Population Therapeutics and Clinical Pharmacology, 26(3), 57–60. https://doi.org/10.53555/jptcp.v26i3.6516