A MOLECULAR ANALYSIS OF ACE GENE (ANGIOTENSIN CONVERTING ENZYME) INSERTION/DELETION POLYMORPHISM IN TOXAEMIA OF PREGNANCY AND NORMOTENSIVE MOTHERS

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Dr.T. Praveen
Dr. Nelapati Swetha Vinela
Dr. Kirankumar P

Keywords

Toxaemia of Pregnancy, Angiotensin Converting Enzyme (ACE), Insertion/Deletion polymorphism(I/D), polymerase chain reaction(PCR), significant p-value

Abstract

Toxaemia of pregnancy is diagnosed in India around 6-10% of all deliveries and is associated with 22% of perinatal foetal deaths and cause 30% of maternal death. Angiotensin Converting Enzyme (ACE) plays a vital role in the Renin Aldosterone System (RAS) which regulates blood pressure by converting Angiotensin-I into a powerful vasoconstrictor Angiotensin-II.The aim of the present study was to explore out the ACE geneI/D polymorphism in cases of toxaemia of pregnancy and normotensive mothers in north India population.Study was done in 100 cases of Toxaemia of pregnancy and 100 controls (Normotensive) pregnant women who were admitted in department of obstetrics and gynecology, Rama Medical College Hospital and Research Centre, Kanpur. 5ml venous blood was collected in EDTA vials and processed for ACE gene I/D polymorphism withusing conventional polymerase chain reaction (PCR). A Deletion polymorphism (D allele) has found to be associated with elevated ACE activity leading to hypertension in pregnant women. On genotype analysis, the frequency of I/I, I/D and D/D polymorphism in controls group were found 40%,34% and 26% respectively. Among cases group, the frequency of I/I, I/D and D/D were recorded 16%,30% and 54% respectively.On comparison of the individual frequencies of the three genotypes, it was found that D/D genotype were more prone to develop disease with an odd’s ratio of 5.192% (D/D vs I/I) and 2.353%(D/D vs I/D).On statistical comparison of D/D genotypes between the controls and cases, the cases showed a significant increase of frequency (p-value=0.0005), an odd’s ratio of 3.341 was obtained against (I/D+I/I) polymorphism.

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References

1. ACOG practice Bulletin. Diagnosis and management of preeclampsia and Eclampsia. obsetgynaecol; 2002; 99: 159-167.
2. Eiland, Elosha, Nzerue, et al., "Preeclampsia 2012". J. of Pregnancy. (2012); 1-7.
3. Fernandoarias. In Practical guide to high risk pregnancy and delivery, Mosby, Harcourtasia private ltd. 2000; 2nd edition: 184-185.
4. Skeggs, LT., Kahn, J. Rand Shumway, N.p. Preparation and function of hypertension converting enzyme. THE Journal of Experimental Medicine. 1956; 103, 295-299.
5. K.K. Griendling, T.J. Murphyand R.W alexander. Molecular biology of the preeclampsia syndrome .Journal of perintal medicine. 2008; Vol.36: No.1, pp. 38-58.
6. C.Hubert, A, M. Houot,P. Corvol, and F.Soubier. Structure of the angiotensin I converting enzyme: Two alternate promoters correspond to evolutionary steps of a duplicated gene,”the Journal of biological chemistry. 1993; vol.87:no.6.pp1816-1828.
7. C MAndo, p.Antonazzo, S.Tabano, Angiotensin converting enzyme and adducing-1 polymorphism in women with preeclampsia and gestational hypertension. Reproductive sciences. 2004; Vol. 16,: No.9,PP.127-131.
8. Katarzyna Kusmierska-Urban. Association of ACE I/D and AGT M235T Gene polymorphism with the Gestational hypertension and the fetal Growth. Obsteric and gynaecology International Journal.2015; March-April.
9. Zohreh Rahimi, Ziba Rahimi, hadiMo Zafari. Preeclampsia and angiotensin converting enzyme (ACE) I/D and angiotensin II type-1 Receptor (AT1R) AII66C polymorphism: association with ACE I/D polymorphism. Journal of the Renin-ngiotensin-aldosterone system. 2012; 14 (2):174-180.
10. Aggarwal S, DimriN, Tandon I, Preeclampsia in North Indian Women: The contribution of genetic polymorphism. J Obsteric Gynaecology Res; 2011:37:1335-1341.
11. Zhen Chen, FangXu, Yonggang Wei, Angiotensin converting enzyme insertion/deletion polymorphism and risk of pregnancy hypertensive disorders: a Meta-Analysis. Journal of the Renin-ngiotensin-aldosterone system. 2011:13(1); 184-195.
12. Uma R, Forsyth SJ, Struthers. Polymorphism of angiotensin converting enzyme Gene in early-onset and late-onset preeclampsia. J Materna fetal Neonatal Med.2010; 23: 874-879.
13. Biller H, Ruprecht B, Gaede KI, et al. Gene polymorphisms of ACE and the angiotensin receptor AT2R1 influence serum ACE levels in sarcoidosis. Sarcoidosis Vasc Diffuse Lung Di. 2009; 26(2): 139-146.
14. Bai H, Liu X, Liu R., et al. Angiotensinogen and angiotensin-I converting enzyme gene variations in Chinese pregnancy induced hypertension. Hua Xi Yi Ke Da Xue Xue Bao; 2002; 33: 233-237.
15. Kaur R, Jain V, Khuller M, et al. Association of angiotensinconverting enzyme gene polymorphism with pregnancyinduced hypertension. Acta Obstet Gynecol Sci.; 2005; 84: 929-933.
16. Seremak-Mrozikiewicz A, Drews K, Malewski Z, et al. Polymorphic genotypes of the angiotensin-convertingenzyme in severe pregnancy-induced hypertension. Arch Perinat Med.2000; 6: 22-24.