PHYSICOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF COLOSTRUM AND MILK UNDER TRANSITION STATE IN KUNDI AND NILI RAVI BUFFALO BREEDS OF PAKISTAN
Main Article Content
Keywords
Colostrum, Transition milk, nutritional attributes, buffalo breeds (Nili Ravi & Kundi)
Abstract
Comparison of physicochemical properties of colostrum and transition milk of two breeds of buffalo (Kundi n=10 and Nili Ravi n= 10, total samples= 220) were investigated. Colostrum and transition milk were obtained 06, 12, 24,36, 48, 60, 72, 84, 96, 108, 120 hours after calving of each animal for analysis. The values of fat %, protein %age, lactose %age, SNF (Solids-Non-Fat)%age, Total solids (TS) %age, ash %age, pH and acidity% of Kundi buffalo colostrum after 6 hours of calving were 8.40±0.06, 13.82±0.33, 2.72±0.14, 20.07±0.01, 28.91±0.01, 1.04±0.10, 6.07±0.00, 0.32±0.00 and of Nili Ravi buffalo colostrum were found as 6.00±0.06, 12.34±0.72, 2.25±0.01, 19.79±0.01, 25.98±0.01, 0.98±0.04, 6.37±0.01 and 0.30±0.01 respectively. Viscosity of Kundi and Nili Ravi Buffaloes (K & NRB) 6.98±0.01b and 7.33±0.01a, respectively at 6 hours, Lysozyme (µg/ml) of buffalo Kundi colostrum after 6 hours of study was 0.32±0.01h and in Nili Ravi (0.34±0.00h). Lactoferrin (mg/ml) was decreased in both breeds of buffalo and at 120 hours after calving it was observed 0.59±0.01j and 0.47±0.01k (K & NRB) transition milk. Statistically results of Analysis of variance of fat %age, lactose %age, SNF %age, TS%, Immunoglobulins (mg/ml), lactoferrin (mg/ml), lysozyme (µg/ml) of (K & NRB) breeds p<0.001 were highly significant.
References
1. Andrew, S. (2001). Composition of colostrum from heifer. Journal of Dairy Science, 84, 100-106.
2. AOAC, (2000). Dairy products. In: official methods of analysis. Association of Official Analytical Chemists lnc, Gatherburg, U.S.A.
3. AOAC, (2005). Official Methods of Analysis of AOAC International. 18th edn. Association of Official Analytical Chemists; Gaithersburg, MD, USA.
4. Arain, H.H., Khaskheli, M., Arain, M.A., Soomro, A.H., & Nizamani, A.H. (2008). Stability and quality characteristics of postpartum buffalo milk. Pakistan Journal of Nutrition, 7(2), 303-307.
5. Aurelia, P., Cristian, C., Camelia, R., Vioara, M., & Gheorghe, M. (2009). The study of the main parameters quality of buffalo milk. African Journal of Microbiology, 10, 201-206.
6. Billakanti, J.M., Fee, C.J., Lane, F.R., Kash, A.S., & Fredericks, R. (2010). Simultaneous, quantitative detection of five whey proteins in multiple samples by surface plasmon resonance Rayleen. International Dairy Journal, 20, 96-105.
7. Błaszkowska, M., & Twardoń, J. (2006). Influence of the total immunoglobulin level in the colostrum and serum of cows on the passive immunity level in calves. Medycyna Weterynaryjna, 62(2), 185-188.
8. Blum, J.W., & Hammon, H. (2000). Colostrum effects on the gastro-intestinal tract, and on nutritional, endocrine and metabolic parameters in neonatal calves. Livestock Production Science, 66(2), 151-159. DOI: 10.1016/S0301/62-26(00)00222-0
9. Campbell, B., & Petersen, W.E. (1963). Immune Milk. A historical survey. Dairy Science, 25, 345-358.
10. Cheng, J.B., Wang, J.Q., Bu, D.P., Liu, G.L., Zhang, C.G., Wei, H.Y., Zhou, L.Y., & Wang, J.Z. (2008). Factors affecting the lactoferrin concentration in bovine milk. Journal of Dairy Science, 91, 970–976.
11. Cziszter, L.T., Acatincăi, S., Stanciu, G., Simona, B., Tripon, I., Silvia, E., & Gavojdian, D. (2008). Study on chemical and biological parameters of colostrum in Romanian Black and White cows. Lucrări ştiinţifice Zootehnie şi Biotehnologii, 41(2), 381-389.
12. Demkowicz, M. (2012). Bioactive preparations from cow colostrum retaining parameters of its nutritional value and its digestibility in lambs and calves. PhD thesis, Uniwersytet Przyrodniczy we Wrocławiu.
13. El-Agamy, E.I., Abou-Shloue, Z.I., & Abdel-Kader, Y.I. (1998). Gel electrophoresis of proteins, physicochemical characterization and vitamin C content of milk of different species. Alexandria Journal of Agricultural Research, 43, 57-70.
14. Elfstrand, L., Lindmark-Mansson, H., Paulsson, M., Nyberg, L, & Akesson, B. (2002). Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effects of processing. International Dairy Journal, 12, 879-887.
15. El-Loly, M.M., & Salim, A. (2008). Specific immune system factors in buffalo’s milk. Proceeding of the 3rd International Conference on Food Science and Nutrition, Nutrition, Nutritional Status, and Food Sciences in Arab Countries, NRC, November 3-5, Cairo, Egypt. 208-219.
16. El-Loly, M.M. (2005). On the carbohydrate moiety of immunoglobulins from buffalo colostrum, milk and blood serum. Milchwissenschaft. 60, 392-395.
17. Fleenor, W.A., & Stott, G.H. (1980). Hydrometer test for estimation of immunoglobulin concentration in bovine colostrum. Journal of Dairy Science, 63, 973–977.
18. Gueorguiev, I.P., Bivolarski, B.L., Kutsarov, G.I., Iliev, Y.I., & Gueorguieva, T. M. (1996). Serum lysozyme activity in new born calves and relationship to milk yield of their mother. Revue de Médecine Vétérinaire. 147, 583–586.
19. Guliński, P., Niedziałek, G., SalaMończyk, E., & Górski, T. (2006). Immunoglobulin content in colostrum of cows within selected genetic and environmental factors. Medycyna Weterynaryjna, 62(3), 339-342.
20. Haggag, H.F., Mahran, C.A., Ali, M.M., & Hamzawi, L.F. (1991). Casein fraction of abnormal Egyptian buffalo milk. Egypt Journal of Dairy Science, 19, 209-219.
21. Hahn, R., Schulz, P.M., Schaupp, C., & Jungbauer, A. (1998). Bovine whey fractionation based on cation-exchange chromatography. Journal of Chromatography A, 795, 277-287.
22. Hofi, A.A., Rifaat, I.D., & Khorshid, M.A. (1966). Studies on some physico-chemical properties of Egyptian buffaloes and cows milk. Indian Journal of Dairy Science, 19, 158-161.
23. Jenness, R., & Patton, S. (1959). Principles of dairy chemistry. John Wiley, New York Kadian, A. (2000). Development of paneer, channa and khees from colostrum. Ph.D. Thesis submitted to Chaudhary Charan Singh Haryana Agricultural University, Haryana, India.
24. Kehoe, S.I., Jayarao, B.M., & Heinrichs, A.J. (2007). A survey of bovine colostrum composition and colostrum management practices on Pennsylvania dairy farms. Journal of Dairy Science, 90(9), 4108-4116. DOI: 10.3168/jds.2007.0040
25. Korohnen, H. (1977). Antimicrobial factors in bovine colostrum. Journal of Agricultural and food Science, 49, 434-447.
26. Kulkarni, P.R., & Pimpale, N.V. (1989). Colostrum – A review. Indian Journal of Dairy Science, 42 (2), 216-224.
27. Levieux, D., & Ollier, A. (1999). Bovine immunoglobulin G, beta-lactoglobulin, alphalactalbumin and serum albumin in colostrum and milk during the early post partum period. Journal of Dairy Research, 66(3), 421-430.
28. Madsen, B.D., Rasmussen, M.D., Nielsen, M.O., Wiking, L., & Larsen, L.B. (2004). Physical properties of mammary secretions in relation to chemical changes during transition from colostrum to milk. Journal of Dairy Research, 71, 263-272.
29. Martin-Sosa, S., Martin, M.J., García-Pardo, & Hueso, P. (2003). "Sialyloligosaccharides in human and bovine milk and in infant formulas: variations with the progression of lactation." Journal of Dairy science, 86(1), 52-59.
30. Nawar, M.A. (2006). Antimicrobial factors in buffalo milk: changes during the first month of lactation. Egyptian Journal of Dairy Science, 34, 133-138.
31. Nickerson, S.C. (1995). Milk Production: Factor Affecting Milk Composition. Blackie Academic and Professionals, UK. Pp, 22-23.
32. Parrish, D.B., Wise, G.H., Highes, J.S., & Atkeson, F.W. (1950). Properties of the colostrum of the dairy cow. V. Yield, specific gravity and concentrations of total solids and its various components of colostrum and early milk. Journal of Dairy Science, 33, 457-465.
33. Pearson, D. (1976). The chemical analysis of foods. 7th edn. Churchill Livingston; London, UK. Pp. 402-449.
34. Prasad, J. (1997). Animal Husbandry and Dairy Science. Kalyani Publishers, New Delhi, India Pages, 316.
35. Prichett, L.C., Gay, C.C., Besser, T.E., & Hancock, D.D. (1991). Management and production factors influencing immunoglobulin G1 concetration in colostrum from Holstein cows. Journal of Dairy Science, 74(7), 2336-2341. DOI: 10.3168/jds.S0022-0302(91)78406-3
36. Quigley, J. (2002). Passive immunity in newborn calves. Advance Dairy Technology, 14, 273-292.
37. Quigley, J.D., Martin, K.R., Dowlen, H.H., Wallis, L.B., & Lamar, K. (1994). Immunoglobulin concentration, specific gravity, and nitrogen fractions of colostrum from Jersey cattle. Journal of Dairy Science, 77, 264-296.
38. Qyeniyi, O.O., & Hunter, A.G. (1978). Colostral constituents including immunoglobulins in the first three milkings postpartum. Journal of Dairy Science, 61(1), 44-48.
39. Reiter, B. (1978). Review of the progress of dairy science: antimicrobial systems in milk. Journal of Dairy Research, 45, 131-147.
40. Richard, E.L. (1959). The reaction of the lactose with anthrone and its application to estimation of lactose in casein and other dairy product. Journal of Dairy Research, 26, 53-57.
41. Rifaat, I.D., Hassan, A., El-Alamy, H.A., & Abd El-Salam, M.H. (1972). The chemical composition and properties of colostrum from buffaloes I. Chemical composition and some physical properties. Egypt Journal of Animal Production, 12, 67-75.
42. Roginski, H., Fuquay, J.W., Fox, P.F. (2003). Colostrum. In: Encyclopedia of Dairy Sciences, Volume 1, Academic Press, London. Pp, 473-478.
43. Roy, J.H.B. (1970). Management and Feeding. Butterworth and Co., Toronto, Canada.
44. Seth, R., & Das, A. (2011). Freeze drying of buffalo colostrum and colostral whey and composition of the dried products. Indian Journal of Dairy Science, 64 (5), 378-382.
45. Singh, A., Chandra, G., Huozha, R., & Kushwaha, R. (2011). Bovine Col Ostrum and Neona Te Immunity - a Review Colostrum Neonate, Agricultural Review, 32, 79-90.
46. Sobczuk-Szul, M., Wielgosz-Groth, Z, Wronski, M., & Rzemieniewski, A. (2013). Changes in the bioactive protein concentrations in the bovine colostrum of Jersey and Polish Holstein-Friesian cows. Turkish Journal of Veterinary and Animal Sciences, 37, 43-49.
47. Steel, R.G.D., Torrie, J.H., & Dickey, D.A. (1997). Principles and Procedures of Statistics. A biometrical approach. 3rd Ed. McGraw Hill Corporation, New York. USA. Pp. 137-175.
48. Strekozov, N.I., Motova, E.N., & Fedorov, Y.N. (2008). Evaluation of the chemical composition and immunological properties of colostrum of cow’s first milk yield. Russian Agricultural Sciences, 34, 259–260.
49. Tripathi, V., & Vashishtha, B. (2006). Bioactive compounds of colostrum and its application. Food Reviews International, 22, 225-244.
50. Tsioulpas, A., Grandison, A.S., & Lewis, M.J. (2007). Changes in physical properties of bovine milk from the colostrum period to early lactation. Journal of Dairy Science, 90(11), 5012–5017.
51. Walstra, P., & Jennes, R. (1984). Dairy Chemistry and Physics. John Wiley and Son Inc., Canada. Pp, 137-138.
52. Zachwieja, A., Szulc, T., Potkańska, A., Mikuła, R., Kruszyński, W., & Dobicki, A. (2007). Effect of different fat suplements used during dry period of cows on colostrum physico-chemical properties. Biotechnology in Animal Husbandry, 23(5-6-2), 67-75. DOI: 10.2298/BAH07020672
2. AOAC, (2000). Dairy products. In: official methods of analysis. Association of Official Analytical Chemists lnc, Gatherburg, U.S.A.
3. AOAC, (2005). Official Methods of Analysis of AOAC International. 18th edn. Association of Official Analytical Chemists; Gaithersburg, MD, USA.
4. Arain, H.H., Khaskheli, M., Arain, M.A., Soomro, A.H., & Nizamani, A.H. (2008). Stability and quality characteristics of postpartum buffalo milk. Pakistan Journal of Nutrition, 7(2), 303-307.
5. Aurelia, P., Cristian, C., Camelia, R., Vioara, M., & Gheorghe, M. (2009). The study of the main parameters quality of buffalo milk. African Journal of Microbiology, 10, 201-206.
6. Billakanti, J.M., Fee, C.J., Lane, F.R., Kash, A.S., & Fredericks, R. (2010). Simultaneous, quantitative detection of five whey proteins in multiple samples by surface plasmon resonance Rayleen. International Dairy Journal, 20, 96-105.
7. Błaszkowska, M., & Twardoń, J. (2006). Influence of the total immunoglobulin level in the colostrum and serum of cows on the passive immunity level in calves. Medycyna Weterynaryjna, 62(2), 185-188.
8. Blum, J.W., & Hammon, H. (2000). Colostrum effects on the gastro-intestinal tract, and on nutritional, endocrine and metabolic parameters in neonatal calves. Livestock Production Science, 66(2), 151-159. DOI: 10.1016/S0301/62-26(00)00222-0
9. Campbell, B., & Petersen, W.E. (1963). Immune Milk. A historical survey. Dairy Science, 25, 345-358.
10. Cheng, J.B., Wang, J.Q., Bu, D.P., Liu, G.L., Zhang, C.G., Wei, H.Y., Zhou, L.Y., & Wang, J.Z. (2008). Factors affecting the lactoferrin concentration in bovine milk. Journal of Dairy Science, 91, 970–976.
11. Cziszter, L.T., Acatincăi, S., Stanciu, G., Simona, B., Tripon, I., Silvia, E., & Gavojdian, D. (2008). Study on chemical and biological parameters of colostrum in Romanian Black and White cows. Lucrări ştiinţifice Zootehnie şi Biotehnologii, 41(2), 381-389.
12. Demkowicz, M. (2012). Bioactive preparations from cow colostrum retaining parameters of its nutritional value and its digestibility in lambs and calves. PhD thesis, Uniwersytet Przyrodniczy we Wrocławiu.
13. El-Agamy, E.I., Abou-Shloue, Z.I., & Abdel-Kader, Y.I. (1998). Gel electrophoresis of proteins, physicochemical characterization and vitamin C content of milk of different species. Alexandria Journal of Agricultural Research, 43, 57-70.
14. Elfstrand, L., Lindmark-Mansson, H., Paulsson, M., Nyberg, L, & Akesson, B. (2002). Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effects of processing. International Dairy Journal, 12, 879-887.
15. El-Loly, M.M., & Salim, A. (2008). Specific immune system factors in buffalo’s milk. Proceeding of the 3rd International Conference on Food Science and Nutrition, Nutrition, Nutritional Status, and Food Sciences in Arab Countries, NRC, November 3-5, Cairo, Egypt. 208-219.
16. El-Loly, M.M. (2005). On the carbohydrate moiety of immunoglobulins from buffalo colostrum, milk and blood serum. Milchwissenschaft. 60, 392-395.
17. Fleenor, W.A., & Stott, G.H. (1980). Hydrometer test for estimation of immunoglobulin concentration in bovine colostrum. Journal of Dairy Science, 63, 973–977.
18. Gueorguiev, I.P., Bivolarski, B.L., Kutsarov, G.I., Iliev, Y.I., & Gueorguieva, T. M. (1996). Serum lysozyme activity in new born calves and relationship to milk yield of their mother. Revue de Médecine Vétérinaire. 147, 583–586.
19. Guliński, P., Niedziałek, G., SalaMończyk, E., & Górski, T. (2006). Immunoglobulin content in colostrum of cows within selected genetic and environmental factors. Medycyna Weterynaryjna, 62(3), 339-342.
20. Haggag, H.F., Mahran, C.A., Ali, M.M., & Hamzawi, L.F. (1991). Casein fraction of abnormal Egyptian buffalo milk. Egypt Journal of Dairy Science, 19, 209-219.
21. Hahn, R., Schulz, P.M., Schaupp, C., & Jungbauer, A. (1998). Bovine whey fractionation based on cation-exchange chromatography. Journal of Chromatography A, 795, 277-287.
22. Hofi, A.A., Rifaat, I.D., & Khorshid, M.A. (1966). Studies on some physico-chemical properties of Egyptian buffaloes and cows milk. Indian Journal of Dairy Science, 19, 158-161.
23. Jenness, R., & Patton, S. (1959). Principles of dairy chemistry. John Wiley, New York Kadian, A. (2000). Development of paneer, channa and khees from colostrum. Ph.D. Thesis submitted to Chaudhary Charan Singh Haryana Agricultural University, Haryana, India.
24. Kehoe, S.I., Jayarao, B.M., & Heinrichs, A.J. (2007). A survey of bovine colostrum composition and colostrum management practices on Pennsylvania dairy farms. Journal of Dairy Science, 90(9), 4108-4116. DOI: 10.3168/jds.2007.0040
25. Korohnen, H. (1977). Antimicrobial factors in bovine colostrum. Journal of Agricultural and food Science, 49, 434-447.
26. Kulkarni, P.R., & Pimpale, N.V. (1989). Colostrum – A review. Indian Journal of Dairy Science, 42 (2), 216-224.
27. Levieux, D., & Ollier, A. (1999). Bovine immunoglobulin G, beta-lactoglobulin, alphalactalbumin and serum albumin in colostrum and milk during the early post partum period. Journal of Dairy Research, 66(3), 421-430.
28. Madsen, B.D., Rasmussen, M.D., Nielsen, M.O., Wiking, L., & Larsen, L.B. (2004). Physical properties of mammary secretions in relation to chemical changes during transition from colostrum to milk. Journal of Dairy Research, 71, 263-272.
29. Martin-Sosa, S., Martin, M.J., García-Pardo, & Hueso, P. (2003). "Sialyloligosaccharides in human and bovine milk and in infant formulas: variations with the progression of lactation." Journal of Dairy science, 86(1), 52-59.
30. Nawar, M.A. (2006). Antimicrobial factors in buffalo milk: changes during the first month of lactation. Egyptian Journal of Dairy Science, 34, 133-138.
31. Nickerson, S.C. (1995). Milk Production: Factor Affecting Milk Composition. Blackie Academic and Professionals, UK. Pp, 22-23.
32. Parrish, D.B., Wise, G.H., Highes, J.S., & Atkeson, F.W. (1950). Properties of the colostrum of the dairy cow. V. Yield, specific gravity and concentrations of total solids and its various components of colostrum and early milk. Journal of Dairy Science, 33, 457-465.
33. Pearson, D. (1976). The chemical analysis of foods. 7th edn. Churchill Livingston; London, UK. Pp. 402-449.
34. Prasad, J. (1997). Animal Husbandry and Dairy Science. Kalyani Publishers, New Delhi, India Pages, 316.
35. Prichett, L.C., Gay, C.C., Besser, T.E., & Hancock, D.D. (1991). Management and production factors influencing immunoglobulin G1 concetration in colostrum from Holstein cows. Journal of Dairy Science, 74(7), 2336-2341. DOI: 10.3168/jds.S0022-0302(91)78406-3
36. Quigley, J. (2002). Passive immunity in newborn calves. Advance Dairy Technology, 14, 273-292.
37. Quigley, J.D., Martin, K.R., Dowlen, H.H., Wallis, L.B., & Lamar, K. (1994). Immunoglobulin concentration, specific gravity, and nitrogen fractions of colostrum from Jersey cattle. Journal of Dairy Science, 77, 264-296.
38. Qyeniyi, O.O., & Hunter, A.G. (1978). Colostral constituents including immunoglobulins in the first three milkings postpartum. Journal of Dairy Science, 61(1), 44-48.
39. Reiter, B. (1978). Review of the progress of dairy science: antimicrobial systems in milk. Journal of Dairy Research, 45, 131-147.
40. Richard, E.L. (1959). The reaction of the lactose with anthrone and its application to estimation of lactose in casein and other dairy product. Journal of Dairy Research, 26, 53-57.
41. Rifaat, I.D., Hassan, A., El-Alamy, H.A., & Abd El-Salam, M.H. (1972). The chemical composition and properties of colostrum from buffaloes I. Chemical composition and some physical properties. Egypt Journal of Animal Production, 12, 67-75.
42. Roginski, H., Fuquay, J.W., Fox, P.F. (2003). Colostrum. In: Encyclopedia of Dairy Sciences, Volume 1, Academic Press, London. Pp, 473-478.
43. Roy, J.H.B. (1970). Management and Feeding. Butterworth and Co., Toronto, Canada.
44. Seth, R., & Das, A. (2011). Freeze drying of buffalo colostrum and colostral whey and composition of the dried products. Indian Journal of Dairy Science, 64 (5), 378-382.
45. Singh, A., Chandra, G., Huozha, R., & Kushwaha, R. (2011). Bovine Col Ostrum and Neona Te Immunity - a Review Colostrum Neonate, Agricultural Review, 32, 79-90.
46. Sobczuk-Szul, M., Wielgosz-Groth, Z, Wronski, M., & Rzemieniewski, A. (2013). Changes in the bioactive protein concentrations in the bovine colostrum of Jersey and Polish Holstein-Friesian cows. Turkish Journal of Veterinary and Animal Sciences, 37, 43-49.
47. Steel, R.G.D., Torrie, J.H., & Dickey, D.A. (1997). Principles and Procedures of Statistics. A biometrical approach. 3rd Ed. McGraw Hill Corporation, New York. USA. Pp. 137-175.
48. Strekozov, N.I., Motova, E.N., & Fedorov, Y.N. (2008). Evaluation of the chemical composition and immunological properties of colostrum of cow’s first milk yield. Russian Agricultural Sciences, 34, 259–260.
49. Tripathi, V., & Vashishtha, B. (2006). Bioactive compounds of colostrum and its application. Food Reviews International, 22, 225-244.
50. Tsioulpas, A., Grandison, A.S., & Lewis, M.J. (2007). Changes in physical properties of bovine milk from the colostrum period to early lactation. Journal of Dairy Science, 90(11), 5012–5017.
51. Walstra, P., & Jennes, R. (1984). Dairy Chemistry and Physics. John Wiley and Son Inc., Canada. Pp, 137-138.
52. Zachwieja, A., Szulc, T., Potkańska, A., Mikuła, R., Kruszyński, W., & Dobicki, A. (2007). Effect of different fat suplements used during dry period of cows on colostrum physico-chemical properties. Biotechnology in Animal Husbandry, 23(5-6-2), 67-75. DOI: 10.2298/BAH07020672
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Jan 23, 2024
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Shakeel Ahmad, Saima Inayat, Muhammad Nadeem, & Ali Ahmad Sheikh. (2024). PHYSICOCHEMICAL AND ANTIMICROBIAL PROPERTIES OF COLOSTRUM AND MILK UNDER TRANSITION STATE IN KUNDI AND NILI RAVI BUFFALO BREEDS OF PAKISTAN. Journal of Population Therapeutics and Clinical Pharmacology, 31(1), 1264–1276. https://doi.org/10.53555/jptcp.v31i1.4169
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Author Biographies
Shakeel Ahmad
Department of Dairy Technology, Faculty of Animal Production & Technology, University of Veterinary and Animal Sciences, Lahore - Pakistan
Saima Inayat
Department of Dairy Technology, Faculty of Animal Production & Technology, University of Veterinary and Animal Sciences, Lahore - Pakistan
Muhammad Nadeem
Department of Dairy Technology, Faculty of Animal Production & Technology, University of Veterinary and Animal Sciences, Lahore - Pakistan
Ali Ahmad Sheikh
University Diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore - Pakistan