Anticancer potential of crude extract and Triterpenoid isolated from Datura metel Linnaeus
Main Article Content
Keywords
Datura metel, extract, Amyrin type triterpenoid, Anticancer
Abstract
Objective:
This research aims to investigate the anticancer properties of a crude extract and a triterpenoid compound isolated from Datura metel Linnaeus. The study explores the diverse chemical composition of D. metel and evaluates the efficacy of the isolated compound against various cancer cell lines.
Methods:
Plant Collection: Seeds of D. metel were obtained, and the plant specimen was identified and deposited.
Extraction and Isolation: Crude extract was obtained using methanol, followed by fractionation. Compound 1 was isolated through thin-layer chromatography and column chromatography.
Anticancer Activity: The MTT procedure was employed to assess cytotoxicity on various cancer cell lines (HepG2, A498, NCI-H226, and MDR 2780AD) using the isolated compound and crude extracts.
Results
Compound 1 exhibited significant anticancer activity compared to the crude extracts across various cell lines. The standard drug, Paclitaxel, demonstrated universal effectiveness. The results show varying IC50 values for hexane, chloroform, methanol extracts, Compound 1, and Paclitaxel against different cell lines.
Discussion
The study emphasizes the increasing importance of natural products in pharmaceutical research, with D. metel identified as a potential source of novel anticancer agents. Compound 1, isolated from D. metel, stands out for its noteworthy efficacy. The findings align with existing literature, suggesting the potential of D. metel extracts in cancer treatment.
Conclusion
The research concludes that Compound 1 from D. metel exhibits significant anticancer potential against various cancer cell lines. The study encourages further exploration of D. metel as a potential reservoir for anticancer compounds, with Compound 1 emerging as a promising candidate for further investigation and pharmaceutical development.
References
1. Firdaus, N.; Viquar, U.; Kazmi, M. H., Potential and pharmacological actions of Dhatura safed (Datura metel L.): as a deadly poison and as a drug: an overview. International Journal of Pharmaceutical Sciences and Research 2020, 11 (7), 3123-3137.
2. Ahmad, Z.; Muhammad, B.; Khan, N., Pharmacokinetics of Natural Compounds: Unlocking the Therapeutic Potential. Phytopharmacology Research Journal 2023, 2 (2), 22-25.
3. Al-Snafi, A. E., Medical importance of Datura fastuosa (syn: Datura metel) and Datura stramonium-A review. IOSR Journal of Pharmacy 2017, 7 (2), 43-58.
4. Monira, K. M.; Munan, S. M., Review on Datura metel: A potential medicinal plant. Global Journal of Research on Medicinal Plants & Indigenous Medicine 2012, 1 (4), 123.
5. Bawazeer, S.; Rauf, A.; Bawazeer, S., Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel’s Trumpet) Fruits. BioMed Research International 2020, 2020.
6. Bawazeer, S.; Rauf, A., In vitro antibacterial and antifungal potential of amyrin-type triterpenoid isolated from Datura metel Linnaeus. BioMed Research International 2021, 2021.
7. Han, X.; Wang, H.; Zhang, Z.; Tan, Y.; Wang, J., Study on chemical constituents in seeds of Datura metel from Xinjiang. Zhong yao cai= Zhongyaocai= Journal of Chinese medicinal materials 2015, 38 (8), 1646-1648.
8. Afsharypuor, S.; Mostajeran, A.; Mokhtary, R., Variation of scopolamine and atropine in different parts of Datura metel during development. Planta medica 1995, 61 (04), 383-384.
9. Tan, J.-Y.; Liu, Y.; Cheng, Y.-G.; Sun, Y.-P.; Li, X.-M.; Guan, W.; Pan, J.; Yang, B.-Y.; Kuang, H.-X., Seven new glycosides from the leaves of Datura metel L. Natural Product Research 2021, 36 (1), 295-304.
10. Alam, W.; Khan, H.; Khan, S. A.; Nazir, S.; Akkol, E. K., Datura metel: A review on chemical constituents, traditional uses and pharmacological activities. Current pharmaceutical design 2021, 27 (22), 2545-2557.
11. Das, S.; Mazumder, A.; Pentela, B., Phytochemical components in Datura metel plant and their therapeutic properties. Allelopathy Journal 2023, 60 (2).
12. Tripathi, Y.; Prabhu, V.; Pal, R.; Mishra, R., Medicinal plants of Rajasthan in Indian system of medicine. Ancient Science of life 1996, 15 (3), 190.
13. Ishola, A. O.; Imam, A.; Ajao, M. S., Effects of datumetine on hippocampal NMDAR activity. Toxicology Reports 2021, 8, 1131-1142.
14. Wijesekara, K. B.; Iqbal, M. C., Induction of Haploid Embryos in Datura metel by Anther Culture. Doubled Haploid Technology: Volume 2: Hot Topics, Apiaceae, Brassicaceae, Solanaceae 2021, 327-336.
15. Wang, Q.; Xiao, H.; Yang, B.; Yao, F.; Kuang, H., Studies on pharmacological actions of the effective parts for psoriasis in Flos Daturae (I). Chinese J Exp Trad Med Formulae 2008, 14, 48-51.
16. Kutama, A.; Mohammed, A.; Kiyawa, S., Hallucinogenic effect of Datura metel L. leaf extract in albino rats. Bioscience Research Communications 2010, 22 (4), 215-220.
17. Naseem, A.; Liu, Y.; Nazli, A.; Kuang, H.-X.; Yang, B.-Y., An Insight Into Indigenous Ethnobotanical and Pharmacological Potential of Solanaceae Family in Pakistan: A Review. Journal of Herbal Medicine 2023, 100763.
18. Kam, P.; Liew, S., Traditional Chinese herbal medicine and anaesthesia. Anaesthesia 2002, 57 (11), 1083-1089.
19. Islam, T.; Ara, I.; Islam, T.; Sah, P. K.; de Almeida, R. S.; Matias, E. F. F.; Ramalho, C. L. G.; Coutinho, H. D. M.; Islam, M. T., Ethnobotanical uses and phytochemical, biological, and toxicological profiles of Datura metel L.: A review. Current Research in Toxicology 2023, 100106.
20. Ogunmoyole, T.; Adeyeye, R. I.; Olatilu, B. O.; Akande, O. A.; Agunbiade, O. J., Multiple organ toxicity of Datura stramonium seed extracts. Toxicology reports 2019, 6, 983-989.
21. Bouzidi, A.; Mahdeb, N.; Kara, N., Toxicity studies of alkaloids of seeds of Datura stramonium and synthesis alkaloids in male rats. J Med Plants Res 2011, 5 (15), 3421-3431.
22. Bawazeer, S.; Rauf, A.; Bawazeer, S., Gastrointestinal motility, muscle relaxation, antipyretic and acute toxicity screening of amyrin type triterpenoid (daturaolone) isolated from Datura metel Linnaeus (Angel’s trumpet) fruits. Frontiers in Pharmacology 2020, 11, 544794.
23. Gomes, C. A.; Girão da Cruz, T.; Andrade, J. L.; Milhazes, N.; Borges, F.; Marques, M. P. M., Anticancer activity of phenolic acids of natural or synthetic origin: a structure− activity study. Journal of medicinal chemistry 2003, 46 (25), 5395-5401.
24. Khan, I.; Nisar, M.; Ahmad, M.; Shah, H.; Iqbal, Z.; Saeed, M.; Halimi, S. M. A.; Kaleem, W. A.; Qayum, M.; Aman, A., Molecular simulations of Taxawallin I inside classical taxol binding site of β-tubulin. Fitoterapia 2011, 82 (2), 276-281.
25. Qayum, M.; Nisar, M.; Rauf, A.; Khan, I.; Kaleem, W. A.; Raza, M.; Karim, N.; Saleem, M. A.; Bawazeer, S.; Uysal, S., In-vitro and in-silico anticancer potential of taxoids from Taxus wallichiana Zucc. Biologia Futura 2019, 70 (4), 295-300.
26. Abrar, H.; Niaz, M.; Ashfaq, S.; Jadoon, R.; Muhammad, B.; Ahmad, Z.; Khan, N., Qualitative phytochemicals profile of five different crude solvents of Galium asperifolium Wall. Phytopharmacology Research Journal 2023, 2 (2), 26-37.
27. Rauf, A.; AlOmar, T. S.; Sarfaraz, S.; Ayub, K.; Hussain, F.; Rashid, U.; Almasoud, N.; AlOmar, A. S.; Rehman, G.; Ahmad, Z., Density functional theory, molecular docking, In vitro and In vivo anti-inflammatory investigation of lapachol isolated from Fernandoa adenophylla. Heliyon 2023.
28. Rauf, A.; Rashid, U.; Atta, A.; Khan, I.; Shah, Z. A.; Mobeen, B.; Javed, A.; Alomar, T. S.; Almasoud, N.; Naz, S., Antiproliferative Activity of Lignans from Olea ferruginea: In Vitro Evidence Supported by Docking Studies. Frontiers in Bioscience-Landmark 2023, 28 (9), 216.
29. Kuriakose, G. C.; Singh, S.; Rajvanshi, P.; Surin, W.; Jayabaskaran, C., In vitro cytotoxicity and apoptosis induction in human cancer cells by culture extract of an endophytic Fusarium solani strain isolated from Datura metel L. Pharm Anal Acta 2014, 5 (2).
30. S. AlOmar, T.; Rauf, A.; Rashid, U.; Sarfaraz, S.; Ayub, K.; Hussain, F.; Almasoud, N.; S. AlOmar, A.; Rehman, G.; Ahmad, Z., Molecular docking, DFT studies, and anti-inflammatory evaluation of peshawaraquinone isolated from Fernandoa adenophylla. Journal of Biomolecular Structure and Dynamics 2023, 1-13.
31. Misawa, M., Plant tissue culture: an alternative for production of useful metabolites. Food & Agriculture Org.: 1994.
32. Inayatullah, S.; Irum, R.; Ateeq-ur-Rehman; Fayyaz Chaudhary, M.; Mirza, B., Biological evaluation of some selected plant species of Pakistan. Pharmaceutical Biology 2007, 45 (5), 397-403.
33. Yadav, S. A.; Koshi, F. S., Phytochemicals from Solanaceae Family and Their Anticancer Properties. In Medicinal Plants, IntechOpen: 2022.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Yousef Hemeq
College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia,