SYNTHESIS, MOLECULAR CHARACTERIZATION OF SILVER NANOPARTICLES AND THEIR ANTIBACTERIAL ACTIVITY AGAINST KLEBSIELLA PNEUMONIAE

Abstract

Klebsiella pneumoniae bacteria have been known for causing a huge number of infections, such as pneumonia, wound or surgical site infections, meningitis, and liver abscesses. Traditionally, Klebsiella pneumoniae is considered to be the causative agent of many serious infections mainly in immunodeficient individuals, but the recent emergence and spread of hypervirulent strains have broadened the number of people susceptible to infections to include those who are healthy and immunosufficient. Moreover, Klebsiella pneumoniae strains have become progressively resistant to a lot of antibiotics treatment protocols and their infections are very challenging to treat. In our research work, we have done an assessment and evaluation of the anti-bacterial action of silver nanoparticles as an alternative for regular treatment protocols for the Klebsiella pneumoniae infections. The bacteria were isolated for soil and the procedures for the identification of bacteria have been achieved through using the both of the biochemical examination and polymerase chain reaction (PCR). The biochemical tests used were included tests like catalase, oxidase, urease, citrate utilization and methyl red test, in addition, polymerase chain reaction has confirmed the identification of the bacteria and the band position of Klebsiella pneumoniae was 130 bp after being visualized on 1.5% agarose gel. With respect to the synthesis and characterization processes of  the silver nanoparticles, the nanoparticles were formed using a green synthesis approach through utilizing the Aspergillus niger to avoid pollution that related to the chemical synthesis approach, to obtain the physicochemical report of  the silver nanoparticles, a number of characterization techniques have been done, like FTIR to acquire the infrared absorption spectrum of the the silver nanoparticles which acts as a fingerprint for the substance, TEM and SEM in the sake of displaying the morphology and giving information about the size and shape of the nanoparticles. With regard to the anti-bacterial activity of silver nanoparticles, agar well diffusion method has been done and the both of MIC and inhibition zone values have been obtained. The values were 156.25 µg/ml and 14 mm respectively.