IN SILICO ANALYSIS OF ARTEMISININ BIOSYNTHETIC GENES AND SEARCH OF METABOLIC PATHWAYS IN OTHER PLANTS
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Keywords
Artemisia annua, biosynthetic pathway, ADH1, ALDH1, AMS1, CYP71AV1, protein-protein interactions
Abstract
One of the most significant drugs for treating malaria is artemisinin, derived from the plant Artemisia annua. Artemisinin has seen a sharp rise in demand over the past ten years due to the development of resistance against other antimalarial drugs. However, natural artemisinin production is meager, which results in a global supply scarcity. Chemical synthesis of it is challenging due to its complicated structure. As a result, Artemisia annua remains the sole plant from which artemisinin is commercially available. Therefore, it is necessary to develop new plans to enhance output or identify alternative sources. In the present study, the multiple sequence alignments, secondary structure analysis, tertiary structure prediction, functional validation and protein-protein interactions of six enzymes namely ADH1, ALDH1, AMS1, CPR, CYP71AV1 and DBR2 were examined. In-silico analysis carried out in this paper has produced promising results.
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Manprit Kaur
Department of Biotechnology, Chaudhary Bansi Lal University, Bhiwani, Haryana, India
Rahul Verma
Department of Biotechnology, Chaudhary Bansi Lal University, Bhiwani, Haryana, India
Arvind Sharma
NIMR, New Delhi
Ashwani Kumar
Department of Biotechnology, Chaudhary Bansi Lal University, Bhiwani, Haryana, India