IMPACT OF AGAVE EXTRACT ON ATHEROSCLEROSIS IN RAT MODEL: ASSESSING ITS THERAPEUTIC POTENTIAL, ELUCIDATING ITS MECHANISMS, IMPLICATIONS FOR CARDIOVASCULAR HEALTH, AND ENHANCING TREATMENT OPTIONS
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Abstract
Atherosclerosis is the deposition of fat within the wall of arteries. Risk factors include increased consumption of saturated fat. In this study, a rat model of atherosclerosis was established using high fat diet, the effect of Agave sisalana L. was analyzed on the experimental model and results were compared with standard drug by biochemical analysis which included lipid profile test and liver function tests. Rats were divided into five groups, all groups except control were given high fat for 8 weeks followed by a treatment for 6 weeks; control (normal rat feed),positive control (high fat diet 1ml/day), drug treated (high fat 1ml/day for 8 weeks + Lipiget 1ml/day for 6 weeks), extract treated (high fat 1ml/day for 8 weeks+ Agave sisalana L. extract 0.5ml/day for 6 weeks) and combined group (high fat 1ml/day for 8 weeks+ Lipiget 0.5ml/day and agave extract 0.25ml/ day for 6 weeks) respectively. Weights were observed on daily basis and a significant increase in weight was observed in all groups fed with high fat diet. After 8 weeks, treatment was given and a decrease in weights were observed in drug treated, extract treated and combined group. Upon biochemical analysis, triglycerides were seen to be high in positive control due to consumption of high fat diet and were decreased in drug, extract and combined groups which shows that both drug and extract have positive effects in reducing atherosclerosis. ALT concentration was low in positive control group and shown to be increased in all treatment groups. Bilirubin was in high concentration in positive control group as compared to other groups. No significant difference was observed in other parameters. Hence, we conclude that consumption of Agave sisalana L. by high fat diet fed rats attenuates the progression of atherosclerotic lesions.
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Ghanwa Amjad
Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad
Aneeza Masood
Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad
Maria Zahid
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore
Andleeb Zahra
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore
Syed Khalil Ullah
Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad
Sania Riaz
Assistant Professor, Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology, Islamabad, Ph. # +923005391330