OVARIAN DAMAGE CAUSED BY HIGH FRUCTOSE CORN SYRUP AND ITS MITIGATIVE EFFECCT WITH ALPHA LIPOIC ACID IN ALBINO MICE (MUS MUSCULUS).

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Hira Ashfaq
Asma Rashid Khan
Muhammad Farhan Nasir
Farah Yasmin
Alia Hussain
Tazeen Hidayat
Zeeshan Haider

Keywords

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Abstract

Present study was based upon the toxic role of High Fructose Corn syrup (HFCS) on the female reproductive organ as well as it focused on the ameliorative role of Alpha lipoid acid (ALA), which ultimately minimizes the toxicity. Biochemical analysis was performed by measuring the levels of Luteinizing- hormone (LH), follicle-stimulating hormone (FSH), Malondialdehyde (MDA) and glutathione (GSH) in blood. Moreover, histopathological damage due to this chemical was also evaluated. There was a significant increase in body weight, reproductive and non-reproductive organ weight, Luteinizing hormone, follicle-stimulating hormone and Malondialdehyde (MDA) levels in dose group CS I in comparison with Control group. While GSH activities were reduced in the dose group CSI, when compared with the control- group. Administration of ALA significantly decreased (p <0.001) body weight, reproductive and non reproductive organ weight, Luteinizing hormone, follicle-stimulating hormone and Malondialdehyde (MDA) levels in the dose group CSII, while GSH value increased in dose group CSII group in comparison with CSI Group. Histological studies showed following defects in the ovaries of dose group CS I mice i.e. oocytes with a loss of contact within the granulosa cells, denatured primordial follicles, Cysts development, degraded oocyte and a lower number of follicles and disarrangements of Tunica albuginea and ovarian surface epithelium. While treatment with ALA reduced the ovarian damage by improving the histopathological changes caused by HFCS. Our results showed that HFCS caused ovarian damages, while ALA may be used as a pharmacological agent against ovarian toxicity induced by HFCS. Key words: High Fructose Corn syrup (HFCS), Alpha lipoid acid (ALA).

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