OPTIMIZED METHANOLIC EXTRACT OF MEDICINALLY IMPORTANT FAGONIA INDICA IMPROVED THE OXIDATIVE STABILITY OF SUNFLOWER OIL AT ACCELERATED STORAGE

Main Article Content

Allah Wadhaya
Ayoub Rashid ch
Sajed Ali
Sadia Bashir
Maqsood Ahmad
Syed Ali Raza
Naveed Iqbal
Muhammad Farhan
Amina Kanwal
Fatima Kabir

Keywords

Fagonia indica, antioxidant, oxidative stability, sunflower oil, accelerated storage, FTIR analysis

Abstract

The oxidative deterioration of vegetable oils is a leading cause to impart various diseases in consumers. The synthetic antioxidants used to stabilize the fats and oils are proved to be toxic for human consumption. Alternatively, medicinal plants provide a significant deal of vital metabolites to encounter food oriented oxidative damages to human body and edible oils. In current study different organic solvents of variable polarity were used to optimize the extraction from Fagonia indica (F. indica) and to evaluate its impact on 1,1-Diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), total phenolic contents (TPC) and total flavonoid contents (TFC). The methanolic extract yielded comparative high extract amount of 24.18±0.36%, TPC 184.17±3.28 mg/g GAE, TFC 102.04±1.02 mg/g RE, DPPH scavenging 94.84±0.28% and FRAP value of 303.55±5.05 TE mM/mL. Being the most potent, methanolic extract was blended with refined, bleached and deodorized sunflower oil in various concentrations. The stabilized sunflower oil samples were stored at 65 ± 2 °C in electric oven for six days and their free fatty acid value, peroxide value, iodine value, ρ-anisidine value, conjugated dienes and trienes were determined after regular interval of 24 hours. The FTIR analysis provided noticeable bands of intensities related to oxidation of sunflower oil to evaluate the extent of deterioration. The values of rancidity parameters were compared with blank and butylated hydroxyanisole blended sunflower oil samples. The methanolic extract of F. indica at 600 mg/kg of oil substantially delayed the oxidative deterioration of sunflower oil during six days heating protocol.  F. indica may be presented as a vital source of novel antioxidant system to enhance the shelf life, oxidative stability and bio-functionality of vegetable oils making it safe for human consumption and health improvement.

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References

1. Rehman, A. Latif, N. Abbas, I. Waheed and M.N. Qaisar. Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F. Tropical Journal of Pharmaceutical Research 18: 791-797 (2019).
2. A.E. El‐Hadary and M. Taha. Pomegranate peel methanolic‐extract improves the shelf‐life of edible‐oils under accelerated oxidation conditions. Food Science and Nutrition 8: 1798-1811(2020).
3. AOCS. 1998. Methods and recommended practices of the American Oil Chemists’ Society. Journal of American Oil Chemists Society. Champaign, IL, USA. 5, 2-93, 1998.
4. Papuc, G. Goran, C. Durdun, V. Nicorescu and G. Stefan. Plant polyphenols as antioxidant and antibacterial agents for shelf-life extension of meat and meat products: classification, structures, sources, and action mechanisms. Comprehensive Reviews in Food Science and Food Safety 16: 1243–1268 (2017).
5. D.O. Kim, S.W. Jeong and C.Y. Lee. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food chemistry81: 321-326 (2003).
6. F. Anwar, H.M. Abdul-Qayyum, A.I. Hussain and S. Iqbal. Antioxidant activity of 100% and 80% methanol extracts from barley seeds (Hordeum vulgare L.): stabilization of
sunflower oil. Grasas y Aceites61: 237–243 (2010).
7. F. Anwar, S.A. Chatha and A.I. Hussain. Assessment of oxidative deterioration of soybean oil at ambient and sunlight storage. Grasas y Aceites 58: 390-395 (2007).
8. F. Azam, N. Sheikh, G. Ali and A. Tayyeb. Fagonia indica repairs hepatic damage through expression regulation of toll-like receptors in a liver injury model.Journal of Immunology Research 2018: (2018). https://doi.org/10.1155/2018/7967135
9. F. Blasi and L. Cossignani. An overview of natural extracts with antioxidant activity for the improvement of the oxidative stability and shelf life of edible oils. Processes 8: 956 (2020).
10. F. Rexhepi, A. Surleva, A. Hyseni, M. Bruçi and Blerina Kodraliu. Comprehensive investigation of thermal degradation characteristics and properties changes of plant edible oils by FTIR-spectroscopy. Acta Chemica Iasi 27: 263-286 (2019).
11. F. Shahidi and Y. Zhong. Antioxidants: regulatory status. Bailey's industrial oil and fat products 15: 491-512 (2005).
12. F. Soriguer, G. Rojo-Martínez, M.C. Dobarganes, J.M. García Almeida, I. Esteva, M. Beltrán, M.S. Ruiz De Adana, F. Tinahones, J.M. Gómez-Zumaquero, E. García-Fuentes and S. González-Romero. Hypertension is related to the degradation of dietary frying oils. American Journal of Clinical Nutrition 78: 1092-1097 (2003).
13. G. Pizzino, N. Irrera, M. Cucinotta, G. Pallio, F. Mannino, V. Arcoraci and A. Bitto. Oxidative stress: Harms and benefits for human health. Oxidative Medicine and Cellular Longevity 2017: (2017). https://doi.org/10.1155/2017/8416763.
14. G. Zengin, Y.S. Cakmak, G.O. Guler and A. Aktumsek. In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from Central Anatolia region of Turkey. Food and Chemical Toxicology 48: 2638-2641 (2010).
15. Orhan, A. Aydin, A. Çölkesen, B. Sener and A.I. Isimer. Free radical scavenging activities of some edible fruit seeds. Pharmaceutical biology 41: 163-165 (2003).
16. I.F. Benzie and J.J. Strain. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry 239: 70-76 (1996).
17. J. William, P. John, M.W. Mumtaz, A.R. Ch, A. Adnan, H. Mukhtar, S. Sharif, S.A. Raza and M.T. Akhtar. Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts. PeerJ 7e:7022 (2019).
18. L.L. Mensor, F.S. Menezes, G.G. Leitão, A.S. Reis, T.C.D. Santos, C.S. Coube and S.G. Leitão. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research15: 127-130 (2001).
19. M. Laguerre, C. Bayrasy, A. Panya, J. Weiss, D.J. McClements, J. Lecomte, E.A. Decker and P. Villeneuve. What makes good antioxidants in lipid-based systems? The next theories beyond the polar paradox. Critical Reviews in Food Science and Nutrition 55: 183–201 (2015).
20. M.R. Loizzo and R. Tundis. Plant Antioxidant for Application in Food and Nutraceutical Industries. Antioxidants (Basel, Switzerland), 8: 453 (2019).
https://doi.org/10.3390/antiox8100453
21. M.U. Farooq, M.W. Mumtaz, H. Mukhtar, U. Rashid, M.T. Akhtar, S.A. Raza and M. Nadeem. UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-methanolic leaf extract of Butea monosperma. Scientific reports 10: 1-14 (2020).
22. P. Liang, C. Chen, S. Zhao, F. Ge, D. Liu, B. Liu, Q. Fan, B. Han and X. Xiong. Application of Fourier Transform Infrared Spectroscopy for the oxidation and peroxide value evaluation in virgin walnut oil. Journal of Spectroscopy 2013: (2013).https://doi.org/10.1155/2013/138728.
23. QD. Do, A.E. Angkawijaya, P.L. Tran-Nguyen, L.H. Huynh, E.F. Soetaredjo, S. Ismadji and Y.H. Ju. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis 22: 296-302 (2014).
24. R.S. Farag, A.Z. Badei and G.S. El Baroty. Influence of thyme and clove essential oils on cottonseed oil oxidation. Journal of American Oil Chemists Society 66: 800-804 (1989).
25. S. Ali, S.A. Chatha, Q. Ali, A.I. Hussain, S.M. Hussain and R. Perveen. Oxidative stability of cooking oil blend stabilized with leaf extract of Eucalyptus citriodora. International Journal of Food Properties 19: 1556-1565 (2016).
26. S. Maurelli, F. Blasi, L. Cossignani, A. Bosi, M.S. Simonetti and P. Damiani. Enzymatic synthesis of structured triacylglycerols containing CLA isomers starting from sn-1, 3-diacylglycerols. Journal of American Oil Chemists Society 86: 127-133 (2009).
27. S. Vichi, Zitterl-Eseer, K. Jugl and M.C. Franz. Determination of the presence of antioxidants derived from sage and organic extracts added to the animal fat by means of assessment of the radical scavenging capacity by photochemilumenescence analysis.
Nahrung 45: 101-104 (2001).
28. S.A. Raza, A. Rashid, J. William and A. Razzaq. Evaluation of oxidative stability of sunflower oil at frying temperature in presence of butylated hydroxyl toluene and methanolic extracts of medicinally important plants of Pakistan. International Food Research Journal 21: 331-334 (2014).
29. S.A. Raza, A. Rehman, A. Adnan and F. Qureshi. Comparison of antioxidant activity of essential oil of Centella asiatica and butylated hydroxyanisole in sunflower oil at ambient conditions. Biharean Biologist 3: 71-75 (2009).
30. S.A. Raza, A.R. Chaudhary, M.W. Mumtaz, A. Adnan, H. Mukhtar and M.T. Akhtar. Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius. Asian Pacific journal of Tropical biomedicine.10: 353-360 (2020).
31. S.A. Raza, M. Ahmad, M.W. Mumtaz, S. Bashir and A.R. Ch. In vitro pharmacological attributes and metabolite's fingerprinting of Conocarpus lancifolius. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 20: 660-671 (2021).
32. S.K. Mishra, P.D. Belur and R. Iyyaswami. Use of antioxidants for enhancing oxidative stability of bulk edible oils: A review. International Journal of Food Science & Technology 56: 1-12 (2021).
33. S.S. Ali, N. Kasoju, A. Luthra, A. Singh, H. Sharanabasava, A. Sahu and U. Bora. Indian medicinal herbs as sources of antioxidants. Food Research International 41: 1-15 (2008).
34. T. Arooj, M. Akhtar, M.A. Raza, S. Muhammad, H. Shabbir and S. Farid, S. Phytochemical profile, antimicrobial, antioxidant, antithelminitic and antiproliferative activities of Asplenium trichomanes L. of Malam Jabba, Swat valley KP, Pakistan. Journal of Population Therapeutics and Clinical Pharmacology 30: 1333-1343 (2023).
35. T. Javed, S.A. Raja, K. Ur Rehman, S. Khalid, N. Khalid and S. Riaz. In silico bimolecular characterization of anticancer phytochemicals from Fagonia indica. Pakistan journal of pharmaceutical sciences 34: 883-889 (2021).
36. Y. Amakura, Y. Umino, S. Tsuji, H. Ito, T. Hatano, T. Yoshida and Y. Tonogai. Constituents and their antioxidative effects in eucalyptus leaf extract used as a natural food additive. Food chemistry 77: 47-56 (2002).
37. Y. Meng, H. Yang, D. Wang, Y. Ma, X. Wang and F. Blasi. Improvement for Oxidative Stability and Sensory Properties of Sunflower Oil Flavored by Huai Chrysanthemum× morifolium Ramat. Essential Oil during Accelerated Storage. Processes 9: 1199 (2021).