Tissue Microrna As a Potential Biomarker in Oral Squamous Cell Carcinoma - A Systematic Review

Main Article Content

Amritha Sripoo.R
Uma Maheswari T.N


miRNA, Oral Squamous Cell Carcinoma, Biomarker


Background: Oral squamous cell carcinoma is the most common form of carcinoma of the oral cavity and ranks as the 12th most common cancer in the world. Cancer development and progression requires inactivation of tumour suppressor gene and activation of proto-oncogenes. Expression of these genes is in part dependent on RNA –based mechanisms. MicroRNAs are essential regulators of diverse cellular processes including proliferation, differentiation ,apoptosis ,survival, motility , invasion and morphogenesis
Aim: To evaluate the differential expression of tissue miRNAs in OSCC tissue samples and their use as biological markers to predict the prognosis of OSCC patients.
Methods: A search was done using MeSH terms and keyword search in the electronic databases namely PubMed, Google Scholar, Cochrane, Science Direct ,Lilacs and additional searches were carried out through cross checking the bibliographies of selected articles. Then based on the inclusion and exclusion criteria and availability of the full texts, a total of 2 articles were included in this systematic review
Result: The search yielded a total of articles out of which 56 articles were included based on the eligibility criteria. Quality assessment based on the Quality Assessment of Diagnostic Accuracy Studies 2 tool was used to obtain a risk of bias chart using Revman 5.4 software and it was proved that from the 2 included studies, one had low risk of bias and another had moderate risk of bias.
Conclusion: This systematic review aimed at improving the current understanding of next generation sequencing and applicability in OSCC. Further, this may be an alleyway for the identification of newer biomarkers using NGS-based technique.

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1. Negrini M, Calin GA, Croce CM. MicroRNA in Human Malignancies. Academic Press; 2022. 432 p.
2. Sekar D, Selvakumar SC, Auxzilia Preethi K. Therapeutic nature of microRNAs in Oral Squamous Cell Carcinoma (OSCC). Oral Oncol. 2022 Nov;134:106106.
3. Chianeh YR, Prabhu K. Biochemical markers in saliva of patients with oral squamous cell carcinoma [Internet]. Vol. 4, Asian Pacific Journal of Tropical Disease. 2014. p. S33–40. Available from: http://dx.doi.org/10.1016/s2222-1808(14)60412-6
4. Maheswari TNU, Venugopal A, Sureshbabu NM, Ramani P. Salivary micro RNA as a potential biomarker in oral potentially malignant disorders: A systematic review. Ci Ji Yi Xue Za Zhi. 2018 Apr;30(2):55–60.
5. Almangush A, Leivo I, Mäkitie AA. Biomarkers for Immunotherapy of Oral Squamous Cell
Carcinoma: Current Status and Challenges. Front Oncol. 2021 Mar 8;11:616629.
6. Ezhilarasan D, Lakshmi T, Subha M, Deepak Nallasamy V, Raghunandhakumar S. The ambiguous role of sirtuins in head and neck squamous cell carcinoma. Oral Dis. 2022 Apr;28(3):559–67.
7. Selvakumar SC, K Auxzilia P, Dinesh Y, Senthilmurugan M, Sekar D. MicroRNA-138 and its targets: A therapeutic molecule for oral squamous cell carcinoma. Oral Oncol. 2022 Jul;130:105925.
8. Sridharan G, Ramani P, Patankar S, Vijayaraghavan R. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019 Apr;48(4):299–306.
9. Website [Internet]. Available from: Sodnom-Ish B, Eo MY, Myoung H, Lee JH, Kim SM. Next generation sequencing-based salivary biomarkers in oral squamous cell carcinoma [Internet]. Vol. 48, Journal of the Korean Association of Oral and Maxillofacial Surgeons. 2022. p. 3–12. Available from: http://dx.doi.org/10.5125/jkaoms.2022.48.1.3
10. Paderno A, Bossi P, Parmar S, Piazza C. Advances in the Multidisciplinary Management of Oral Cancer. Frontiers Media SA; 2022. 280 p.
11. Teja KV, Ramesh S, Priya V. Regulation of matrix metalloproteinase-3 gene expression in inflammation: A molecular study. J Conserv Dent. 2018 Nov-Dec;21(6):592–6.
12. Kannan B, Arumugam P. Long non-coding RNAs as a therapeutic target for periodontitis. J Dent Sci. 2022 Oct;17(4):1839–40.
13. Uma Maheswari TN, Nivedhitha MS, Ramani P. Expression profile of salivary micro RNA-21 and 31 in oral potentially malignant disorders. Braz Oral Res. 2020 Feb 10;34:e002.
14. Condrat CE, Thompson DC, Barbu MG, Bugnar OL, Boboc A, Cretoiu D, et al. miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis. Cells [Internet]. 2020 Jan 23;9(2). Available from: http://dx.doi.org/10.3390/cells9020276
15. Nylander K. Squamous Cell Carcinoma of the Head and Neck: Proliferation, P53 and Prognosis. 1995. 114 p.
16. Sekar D, Murthykumar K, Ganapathy D. miR-206 and its mimics: A predictive biomarker and therapeutic molecule in the treatment of oral cancer. Oral Oncol. 2022 May;128:105849.
17. Haribhakti VV. Restoration, Reconstruction and Rehabilitation in Head and Neck Cancer. Springer; 2019. 354 p.
18. Kirita T, Omura K. Oral Cancer: Diagnosis and Therapy. Springer; 2015. 426 p.
19. Troiano G, Mastrangelo F, Caponio VCA, Laino L, Cirillo N, Lo Muzio L. Predictive Prognostic Value of Tissue-Based MicroRNA Expression in Oral Squamous Cell Carcinoma: A Systematic Review and Meta-analysis [Internet]. Vol. 97, Journal of Dental Research. 2018. p. 759–66. Available from: http://dx.doi.org/10.1177/0022034518762090
20. Krishnamoorthy K, Priya Veeraraghavan V, Jayaraman S. E3 ubiquitin ligases: A therapeutic drug target in head and neck squamous cell carcinoma. Oral Oncol. 2022 Dec;135:106193.
21. Sundramoorthy AK, Atchudan R. Analysis of circulating tumor cells (CTCs) using biosensors
made of conducting polymer, poly(3,4-ethylenedioxythiophene), with antifouling properties in human blood. Oral Oncol. 2022 Nov;134:106138.
22. Sankar S. New insights into CAR T-cell therapy for recurrent head and neck squamous cell carcinoma. Oral Oncol. 2022 Nov;134:106137.
23. Prasad M, Jayaraman S, Rajagopal P, Veeraraghavan VP. Apoptosis regulating CircRNAs: Emerging role in oral cancer therapeutics. Oral Oncol. 2022 Nov;134:106107.
24. Paramasivam A. Circulating circular RNAs: Novel potential biomarkers and therapeutic targets for oral cancer. Oral Oncol. 2022 Nov;134:106067.