Evaluation Of the Degradation, Microbial Colonization, Sustainability of Physical and Mechanical Properties of Lycopene Coated Suture Materials - An In-Vitro Study
Main Article Content
Keywords
lycopene, suture material, wound healing, periodontal surgery, antioxidants
Abstract
Background: Lycopene has biological properties and is widely used in medicine these days. The purpose of this study was to compare the effects of lycopene, on the physical and mechanical properties of the suture material, which is commonly used in periodontal, oral and maxillofacial surgeries.
Material and Method: The lycopene gel was prepared and the suture material was dipped in it for 24 hrs. Tensile strength, wound healing assay were evaluated. The presence of lycopene was confirmed using SEM analysis.
Result: The lycopene coating was well adhered to the suture and results obtained by the wound healing assay demonstrated that the presence of lycopene promoted cell migration and proliferation in the wound area suggesting an active role of lycopene in the wound healing process. The tensile strength was stable after 24 hrs.
Conclusion: This research showed that the antioxidant coatings developed on silk sutures may offer important advantages in terms of prevention from surgical infections and wound healing process, and suggested a novel approach towards antioxidant biomaterials for clinical practice.
References
1. Kakoei S, Baghaei F, Dabiri S, Parirokh M, Kakooei S. A comparative in vivo study of tissue reactions to four suturing materials. Iran Endod J. 2010 May 20;5(2):69–73.
2. Minozzi F, Bollero P, Unfer V, Dolci A, Galli M. The sutures in dentistry. Eur Rev Med Pharmacol Sci. 2009 May-Jun;13(3):217–26.
3. Javed F, Al-Askar M, Almas K, Romanos GE, Al-Hezaimi K. Tissue reactions to various suture materials used in oral surgical interventions. ISRN Dent. 2012 May 8;2012:762095.
4. Petrie E. Handbook of Adhesives and Sealants. McGraw Hill Professional; 2007. 800 p.
5. Sridharan G, Ramani P, Patankar S, Vijayaraghavan R. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019 Apr;48(4):299–306.
6. Duraisamy R, Krishnan CS, Ramasubramanian H, Sampathkumar J, Mariappan S, Navarasampatti Sivaprakasam A. Compatibility of Nonoriginal Abutments With Implants: Evaluation of Microgap at the Implant-Abutment
Interface, With Original and Nonoriginal Abutments. Implant Dent. 2019 Jun;28(3):289–95.
7. Vijayakumar Jain S, Muthusekhar MR, Baig MF, Senthilnathan P, Loganathan S, Abdul Wahab PU, et al. Evaluation of Three-Dimensional Changes in Pharyngeal Airway Following Isolated Lefort One Osteotomy for the Correction of Vertical Maxillary Excess: A Prospective Study. J Maxillofac Oral Surg. 2019 Mar;18(1):139–46.
8. Prabakar J, John J, Arumugham IM, Kumar RP, Sakthi DS. Comparing the Effectiveness of Probiotic, Green Tea, and Chlorhexidine- and Fluoride-containing Dentifrices on Oral Microbial Flora: A Double-blind, Randomized Clinical Trial. Contemp Clin Dent. 2018 Oct-Dec;9(4):560–9.
9. Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative Evaluation of Bone Formation between PRF and Blood Clot Alone as the Sole Sinus-Filling Material in Maxillary Sinus Augmentation with the Implant as a Tent Pole: A Randomized Split-Mouth Study. J Long Term Eff Med Implants. 2019;29(2):105–11.
10. Ramesh A, Varghese S, Jayakumar ND, Malaiappan S. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018 Oct;89(10):1241–8.
11. Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019 Nov;10(4):e12440.
12. Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018 Sep-Oct;29(5):588–93.
13. Lambris JD, Mastellos DC, Reis ES. Therapeutic Modulation of the Complement System: Clinical Indications and Emerging Drug Leads. Frontiers Media SA; 2020. 185 p.
14. Rajasekar A, Varghese SS. Bacterial Profile Associated with Peri-Implantitis: A Systematic Review. J Long Term Eff Med Implants. 2023;33(3):9–20.
15. Website [Internet]. Available from: doi: http://dx.doi.org/10.19070/2377-8075-21000779
16. Mony U, Priya Veeraraghavan V. “Rules” to the genetic progression of tumours deciphered: Is it time to think differently in treating oral cancer patients? Oral Oncol. 2022 Nov;134:106111.
17. Govindarasu M, Prathap L, Govindasamy R. Histone deacetylase inhibitors regulate the oral cancer via PI3K/AKT signaling pathway. Oral Oncol. 2022 Dec;135:106221.
18. Jabin Z, Jain G, Jaiswal M, Vishnu Priya V. Top 100 cited articles on Silver diamine fluoride-A bibliometric analysis. J Oral Biol Craniofac Res. 2022 May 18;12(4):413–20.
19. Devi SS, Dinesh S, Sivakumar A, Nivethigaa B, Alshehri A, Awadh W, et al. Reliability of Frankfort Horizontal Plane with True Horizontal Plane in Cephalometric Measurements. J Contemp Dent Pract. 2022 Sep 23;23(6):601–5.
20. Garapati B, Ramamurthy J, Shanmugam R. Formulation, development, and evaluation of anti-inflammatory and antimicrobial effects of a novel polyherbal mouthwash-An study. J Popul Ther Clin Pharmacol. 2022 Aug 15;29(3):e94–103.
21. Garapati B, Malaiappan S, Rajeshkumar S, Murthykumar K. Cytotoxicity of lycopene-mediated silver nanoparticles in the embryonic development of zebrafish-An animal study. J Biochem Mol Toxicol. 2022 Oct;36(10):e23173.
22. Chandra RV, Sandhya YP, Nagarajan S, Reddy BH, Naveen A, Murthy KRV. Efficacy of lycopene as a locally delivered gel in the treatment of chronic periodontitis: smokers vs nonsmokers. Quintessence Int. 2012 May;43(5):401–11.
23. Kornman KS, Page RC, Tonetti MS. The host response to the microbial challenge in periodontitis: assembling the players. Periodontol 2000. 1997 Jun;14:33–53.
24. Król K. [Reactive oxygen species and antioxidant mechanisms in the pathogenesis of periodontitis]. Ann Acad Med Stetin. 2004;50(2):135–48.
25. Waddington RJ, Moseley R, Embery G. Reactive oxygen species: a potential role in the pathogenesis of periodontal diseases. Oral Dis. 2000 May;6(3):138–51.
26. Gallo AL, Paladini F, Romano A, Verri T, Quattrini A, Sannino A, et al. Efficacy of silver coated surgical sutures on bacterial contamination, cellular response and wound healing. Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:884–93.
27. Lu Y, Edwards A, Chen Z, Tseng TS, Li M, Gonzalez GV, et al. Insufficient Lycopene Intake Is Associated With High Risk of Prostate Cancer: A Cross-Sectional Study From the National Health and Nutrition Examination Survey (2003-2010). Front Public Health. 2021 Dec 13;9:792572.
28. Stahl W. Carrots, tomatoes and cocoa: Research on dietary antioxidants in Düsseldorf. Arch Biochem Biophys. 2016 Apr 1;595:125–31.
29. Giovannucci E. Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J Natl Cancer Inst. 1999 Feb 17;91(4):317–31.
30. Stone V, Johnston HJ, Balharry D, Gernand JM, Gulumian M. Approaches to Develop Alternative Testing Strategies to Inform Human Health Risk Assessment of Nanomaterials. Risk Anal. 2016 Aug;36(8):1538–50.
31. Aslantürk ÖS. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. 2018.
32. Vivek M, Kumar PS, Steffi S, Sudha S. Biogenic Silver Nanoparticles by Gelidiella acerosa Extract and their Antifungal Effects. Avicenna J Med Biotechnol. 2011 Jul;3(3):143–8.
33. Hauman CHJ, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 1. Intracanal drugs and substances. Int Endod J. 2003 Feb;36(2):75–85.
34. Balekar N, Nakpheng T, Katkam NG, Srichana T. Wound healing activity of ent-kaura-9(11),16-dien-19-oic acid isolated from Wedelia trilobata (L.) leaves. Phytomedicine. 2012 Oct 15;19(13):1178–84.
35. Liang Y. The Role of Nuclear Pore Proteins in Cell Differentiation. 2013. 132 p.
36. Günter CI, Machens HG. New strategies in clinical care of skin wound healing. Eur Surg Res. 2012 Jul 11;49(1):16–23.
37. Schäfer S, Smeets R, Köpf M, Drinic A, Kopp A, Kröger N, et al. Antibacterial properties of functionalized silk fibroin and sericin membranes for wound healing applications in oral and maxillofacial surgery. Biomater Adv. 2022 Apr;135:212740.
38. Liu X. The Role of Silver Nanoparticles on Skin Wound Healing, Tissue Remodeling and Their Potential Cytotoxicity. Open Dissertation Press; 2017.
2. Minozzi F, Bollero P, Unfer V, Dolci A, Galli M. The sutures in dentistry. Eur Rev Med Pharmacol Sci. 2009 May-Jun;13(3):217–26.
3. Javed F, Al-Askar M, Almas K, Romanos GE, Al-Hezaimi K. Tissue reactions to various suture materials used in oral surgical interventions. ISRN Dent. 2012 May 8;2012:762095.
4. Petrie E. Handbook of Adhesives and Sealants. McGraw Hill Professional; 2007. 800 p.
5. Sridharan G, Ramani P, Patankar S, Vijayaraghavan R. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019 Apr;48(4):299–306.
6. Duraisamy R, Krishnan CS, Ramasubramanian H, Sampathkumar J, Mariappan S, Navarasampatti Sivaprakasam A. Compatibility of Nonoriginal Abutments With Implants: Evaluation of Microgap at the Implant-Abutment
Interface, With Original and Nonoriginal Abutments. Implant Dent. 2019 Jun;28(3):289–95.
7. Vijayakumar Jain S, Muthusekhar MR, Baig MF, Senthilnathan P, Loganathan S, Abdul Wahab PU, et al. Evaluation of Three-Dimensional Changes in Pharyngeal Airway Following Isolated Lefort One Osteotomy for the Correction of Vertical Maxillary Excess: A Prospective Study. J Maxillofac Oral Surg. 2019 Mar;18(1):139–46.
8. Prabakar J, John J, Arumugham IM, Kumar RP, Sakthi DS. Comparing the Effectiveness of Probiotic, Green Tea, and Chlorhexidine- and Fluoride-containing Dentifrices on Oral Microbial Flora: A Double-blind, Randomized Clinical Trial. Contemp Clin Dent. 2018 Oct-Dec;9(4):560–9.
9. Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative Evaluation of Bone Formation between PRF and Blood Clot Alone as the Sole Sinus-Filling Material in Maxillary Sinus Augmentation with the Implant as a Tent Pole: A Randomized Split-Mouth Study. J Long Term Eff Med Implants. 2019;29(2):105–11.
10. Ramesh A, Varghese S, Jayakumar ND, Malaiappan S. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018 Oct;89(10):1241–8.
11. Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019 Nov;10(4):e12440.
12. Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018 Sep-Oct;29(5):588–93.
13. Lambris JD, Mastellos DC, Reis ES. Therapeutic Modulation of the Complement System: Clinical Indications and Emerging Drug Leads. Frontiers Media SA; 2020. 185 p.
14. Rajasekar A, Varghese SS. Bacterial Profile Associated with Peri-Implantitis: A Systematic Review. J Long Term Eff Med Implants. 2023;33(3):9–20.
15. Website [Internet]. Available from: doi: http://dx.doi.org/10.19070/2377-8075-21000779
16. Mony U, Priya Veeraraghavan V. “Rules” to the genetic progression of tumours deciphered: Is it time to think differently in treating oral cancer patients? Oral Oncol. 2022 Nov;134:106111.
17. Govindarasu M, Prathap L, Govindasamy R. Histone deacetylase inhibitors regulate the oral cancer via PI3K/AKT signaling pathway. Oral Oncol. 2022 Dec;135:106221.
18. Jabin Z, Jain G, Jaiswal M, Vishnu Priya V. Top 100 cited articles on Silver diamine fluoride-A bibliometric analysis. J Oral Biol Craniofac Res. 2022 May 18;12(4):413–20.
19. Devi SS, Dinesh S, Sivakumar A, Nivethigaa B, Alshehri A, Awadh W, et al. Reliability of Frankfort Horizontal Plane with True Horizontal Plane in Cephalometric Measurements. J Contemp Dent Pract. 2022 Sep 23;23(6):601–5.
20. Garapati B, Ramamurthy J, Shanmugam R. Formulation, development, and evaluation of anti-inflammatory and antimicrobial effects of a novel polyherbal mouthwash-An study. J Popul Ther Clin Pharmacol. 2022 Aug 15;29(3):e94–103.
21. Garapati B, Malaiappan S, Rajeshkumar S, Murthykumar K. Cytotoxicity of lycopene-mediated silver nanoparticles in the embryonic development of zebrafish-An animal study. J Biochem Mol Toxicol. 2022 Oct;36(10):e23173.
22. Chandra RV, Sandhya YP, Nagarajan S, Reddy BH, Naveen A, Murthy KRV. Efficacy of lycopene as a locally delivered gel in the treatment of chronic periodontitis: smokers vs nonsmokers. Quintessence Int. 2012 May;43(5):401–11.
23. Kornman KS, Page RC, Tonetti MS. The host response to the microbial challenge in periodontitis: assembling the players. Periodontol 2000. 1997 Jun;14:33–53.
24. Król K. [Reactive oxygen species and antioxidant mechanisms in the pathogenesis of periodontitis]. Ann Acad Med Stetin. 2004;50(2):135–48.
25. Waddington RJ, Moseley R, Embery G. Reactive oxygen species: a potential role in the pathogenesis of periodontal diseases. Oral Dis. 2000 May;6(3):138–51.
26. Gallo AL, Paladini F, Romano A, Verri T, Quattrini A, Sannino A, et al. Efficacy of silver coated surgical sutures on bacterial contamination, cellular response and wound healing. Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:884–93.
27. Lu Y, Edwards A, Chen Z, Tseng TS, Li M, Gonzalez GV, et al. Insufficient Lycopene Intake Is Associated With High Risk of Prostate Cancer: A Cross-Sectional Study From the National Health and Nutrition Examination Survey (2003-2010). Front Public Health. 2021 Dec 13;9:792572.
28. Stahl W. Carrots, tomatoes and cocoa: Research on dietary antioxidants in Düsseldorf. Arch Biochem Biophys. 2016 Apr 1;595:125–31.
29. Giovannucci E. Tomatoes, tomato-based products, lycopene, and cancer: review of the epidemiologic literature. J Natl Cancer Inst. 1999 Feb 17;91(4):317–31.
30. Stone V, Johnston HJ, Balharry D, Gernand JM, Gulumian M. Approaches to Develop Alternative Testing Strategies to Inform Human Health Risk Assessment of Nanomaterials. Risk Anal. 2016 Aug;36(8):1538–50.
31. Aslantürk ÖS. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. 2018.
32. Vivek M, Kumar PS, Steffi S, Sudha S. Biogenic Silver Nanoparticles by Gelidiella acerosa Extract and their Antifungal Effects. Avicenna J Med Biotechnol. 2011 Jul;3(3):143–8.
33. Hauman CHJ, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 1. Intracanal drugs and substances. Int Endod J. 2003 Feb;36(2):75–85.
34. Balekar N, Nakpheng T, Katkam NG, Srichana T. Wound healing activity of ent-kaura-9(11),16-dien-19-oic acid isolated from Wedelia trilobata (L.) leaves. Phytomedicine. 2012 Oct 15;19(13):1178–84.
35. Liang Y. The Role of Nuclear Pore Proteins in Cell Differentiation. 2013. 132 p.
36. Günter CI, Machens HG. New strategies in clinical care of skin wound healing. Eur Surg Res. 2012 Jul 11;49(1):16–23.
37. Schäfer S, Smeets R, Köpf M, Drinic A, Kopp A, Kröger N, et al. Antibacterial properties of functionalized silk fibroin and sericin membranes for wound healing applications in oral and maxillofacial surgery. Biomater Adv. 2022 Apr;135:212740.
38. Liu X. The Role of Silver Nanoparticles on Skin Wound Healing, Tissue Remodeling and Their Potential Cytotoxicity. Open Dissertation Press; 2017.
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Published
May 13, 2023
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Bhavana Garapati, Sankari Malaiappan, & Paladugu Devi Navya. (2023). Evaluation Of the Degradation, Microbial Colonization, Sustainability of Physical and Mechanical Properties of Lycopene Coated Suture Materials - An In-Vitro Study. Journal of Population Therapeutics and Clinical Pharmacology, 30(10), 101–107. https://doi.org/10.47750/jptcp.2023.30.10.015
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