PCT, CPT, S-TREM1 and Neopetrin as inflammatory biomarkers Detection, DNA sequencing, 3D Protein and recording a new gene in cancer children with Respiratory tract infection

Main Article Content

Azhar AL-Musawi
Ihsan E. Alsaimary
Hussam M. Salih

Keywords

Biomarkers, genetic, cancer, RIT, protein, genes, DNA sequencing

Abstract

The aim of this study was to determine molecular detection of inflammatory biomarkers (PCT, CPT, S-TREM1 and Neopetrin) by using DNA sequencing, 3D Protein to give acknowledge about the roles of these biomarkers among cancer’s patients with Respiratory tract Basrah province. DNA sequencing of PCT, CPT, S-TREM1, and Neopetrin has shown that there is a convergence between studied PCT and that of the Gen Bank database (NCBI) with identity 91/96 (95%) in forward PCT. A case-control study included 100 confirmed cancer's patients with Respiratory infection and 100 children as a control group. There was a convergence between studied CPT isolated and that of the Gen Bank database (NCBI) with identity 186/190(98%) in forward CPT that had been recorded four mutations appeared in (GGG to GGC), (GTA to GTC), (GGT to GGG) and (CC-to CCC). The forward CPT shown an identity144/145(99%) when compared with Gen Bank database (NCBI) one mutations reported as (CC-to CCC), (TCT to TCG), (GT-to GTG) and (TG-to TGG) in isolated s-trem1 that had been recorded. The reverse CPT had an identity 344/349 (99%), which is similar to that of the Gen Bank data. There was a convergence between studied Neopeterin isolated and that of the GenBank database (NCBI) with identity 166/167(99%) in forward Neopetrin that had been recorded one mutation appeared in (TGA to TG-). The reverse s-trem1 shown an identity 327/328(99) when compared with Gen Bank database that found one mutations was, (CGC to CG-). Neopetrin shown an identity 166/169(98%) when compared with Gen Bank database (NCBI) three mutations reported as (GGT to GGG), (TG- to TGT), (GA to GA-) and (TGA to TG-). No previous studies about the same this mutation in biomarkers. The present study sequencing of PCT, CPT, S-TREM1 and Neopetrin that showed partial a convergence between study PCT isolated and that of the GenBank database (NCBI) with identity 47/47(100%) PCT. A four mutations appeared as (S to P), (Y to S), (Ito GAB) and (R to Q). There convergence between study S-TREM1 isolated and that of the GenBank database (NCBI) with identity 71/74(96%) in S- TREM1 had a three mutations appeared as (M to I), (I to G) and (E to D). No previous studies interested studied in 3D protein with biomarkers. Neopetrin isolated and N-methylenyl methionine isolated showed high convergence between the two, with identity 103/104 (99%) and 99% convergence.

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References

1. Aung, R. Lower Respiratory Tract Infections In Multiple Subgroups Of Immunocompromised Children: A Retrospective Cohort Study, 2020.
2. Campbell, S. & Forbes, B. A. The Clinical Microbiology Laboratory In The Diagnosis Of Lower Respiratory Tract Infections. Journal Of Clinical Microbiology. 2011, 49, S30-S33.
3. Kesson, A. M. & Kakakios, A. Immunocompromised Children: Conditions And Infectious Agents. Paediatric Respiratory Reviews. 2007, 8, 231-239.
4. Li, Q., Meng, H., Zhang, L., Xu, L., Chen, Z., Shi, D., Feng, X., Zhu, X., Zhao, H. & Cao, C. Correlation Between Single Nucleotide Polymorphisms In A Calprotectin Subunit Gene And Risk Of Periodontitis In A Chinese Population. Annals Of Human Genetics. 2007, 71, 312-324.
5. Mohan, A. & Harikrishna, J. Biomarkers for the diagnosis of bacterial infections: in pursuit of the ‘Holy Grail’. The Indian journal of medical research. 2015, 141, 271.
6. Niederman, M. S. Biological markers to determine eligibility in trials for communityacquired pneumonia: a focus on procalcitonin. Clinical infectious diseases. 2008, 47, S127-S132.
7. PENG, L.-S., LI, J., ZHOU, G.-S., DENG, L.-H. & YAO, H.-G. Relationships between genetic polymorphisms of triggering receptor expressed on myeloid cells-1 and septic shock in a Chinese Han population. World Journal of Emergency Medicine. 2015,, 6, 123.
8. Rivera-Chávez, F. A., Huebinger, R. M., Burris, A., Liu, M.-M., Minei, J. P., Hunt, J. L., Arnoldo, B. D. & Barber, R. C. A Trem-1 Polymorphism A/T Within The Exon 2 Is Associated With Pneumonia In Burn-Injured Patients. International Scholarly Research Notices, 2013.
9. Savva, A., Plantinga, T. S., Kotanidou, A., Farcas, M., Baziaka, F., Raftogiannis, M., Orfanos, S. E., Dimopoulos, G., Netea, M. G. & Giamarellos-Bourboulis, E. J. Association Of Autophagy-Related 16-Like 1 (Atg16l1) Gene Polymorphism With Sepsis Severity In Patients With Sepsis And Ventilatorassociated Pneumonia. European Journal Of Clinical Microbiology & Infectious Diseases. 2014, 33, 1609-1614.
10. Zadeh, Firoozeh Abolhasani, Et Al. Cytotoxicity Evaluation Of Environmentally Friendly Synthesis Copper/Zinc Bimetallic Nanoparticles On Mcf-7 Cancer Cells. Rendiconti Lincei. Scienze Fisiche E Naturali, 2022, 1-7.
11. Rohmah, Martina Kurnia, Et Al. Modulatory Role Of Dietary Curcumin And Resveratrol On Growth Performance, Serum Immunity Responses, Mucus Enzymes Activity, Antioxidant Capacity And Serum And Mucus Biochemicals In The Common Carp, Cyprinus Carpio Exposed To Abamectin. Fish & Shellfish Immunology, 2022, 129: 221-230.
12. Arif, Anam, Et Al. The Functions And Molecular Mechanisms Of Tribbles Homolog 3 (Trib3) Implicated In The Pathophysiology Of Cancer. International Immunopharmacology, 2023, 114: 109581.
13. Margiana, Ria, Et Al. Functions And Therapeutic Interventions Of Non-Coding Rnas Associated With Tlr Signaling Pathway In Atherosclerosis. Cellular Signalling, 2022, 100: 110471.
14. H. A. Al-Hchaimi, M. F. Alhamaidah, H. Alkhfaji, M. T. Qasim, A. H. Al-Nussairi And H. S. Abd-Alzahra, "Intraoperative Fluid Management For Major Neurosurgery: Narrative Study," 2022 International Symposium On Multidisciplinary Studies And Innovative Technologies (Ismsit), 2022, Pp. 311-314, Doi: 10.1109/Ismsit56059.2022.9932659.
15. Mohammed, Zainab; Qasim, Maytham T. The Relationship Between Insulin Resistance And Hypertension In Patient With Hypertensive. Hiv Nursing, 2022, 22.2: 1659–1663-1659–1663.
16. Lei, Zimeng, Et Al. Detection Of Abemaciclib, An Anti-Breast Cancer Agent, Using A New Electrochemical Dna Biosensor. Frontiers In Chemistry, 2022, 10.
17. Bashar, Bashar S., Et Al. Application Of Novel Fe3o4/Zn-Metal Organic Framework Magnetic Nanostructures As An Antimicrobial Agent And Magnetic Nanocatalyst In The Synthesis Of Heterocyclic Compounds. Frontiers In Chemistry, 2022, 10.
18. Schoe, A., De Jonge, E., Klautz, R. J., Van Dissel, J. T. & Van De Vosse, E. Single-Nucleotide Polymorphisms In The Calca Gene Are Associated With Variation Of Procalcitonin Concentration In Patients Undergoing Cardiac Surgery. American Journal Of Respiratory And Critical Care Medicine. 2016, 194, 767-769.
19. Schuetz, P., Christ-Crain, M. & Muller, B. Procalcitonin And Other Biomarkers To Improve Assessment And Antibiotic Stewardship In Infections--Hope For Hype? Swiss Medical Weekly. 2009, 139, 318.
20. Seir, R. A., Najajrah, R., Najjar, D., Ashour, M., Asakra, B., Samman, N. & Najjar, O. Acute Respiratory Tract Infections Among Hospitalized Palestinian Patients: A Retrospective Study, 2019.
21. Summah, H. & Qu, J.-M. Biomarkers: A Definite Plus In Pneumonia. Mediators Of Inflammation, 2009.
22. Treatment, W. S. A. R. I. Centre Practical Manual To Set Up And Manage A Sari Treatment Centre And A Sari Screening Facility In Health Care Facilities. Geneva: World Health Organization, 2020.