EVALUATION OF ANTI-CANCEROUS ACTIVITY OF ASPLENIUM DALHOUSIAE HOOK. AND ASPLENIUM TRICHOMANES L. AGAINST MCF 7 AND HCT 116 CELL LINES
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Keywords
Anticancerous, antiproliferative, growth inhibition, Asplenium trichomanes L., Asplenium dalhousiae, MCF 7, HCT 116.
Abstract
Asplenium dalhousiae Hook. and Asplenium trichomanes L. are used in traditional medicine, but insufficient data is available on their anticancerous activity. The extracts of both ferns were tested against human cancer cell lines viz. MCF 7 (breast carcinoma) and HCT 116 (Human colorectal carcinoma. The activity was analyzed employing MTT assay to assess growth inhibition and cell viability percentages. This method measures the reduction of yellow MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide) by mitochondrial succinate dehydrogenase to form dark blue formazan which is largely specific to a viable cell and can be extracted with an organic solvent and then quantified by spectrophotometry. Results were compared with standard anticancerous drug doxorubicin. The results thus obtained showed selective anticancerous activity. Species-specific effect was observed, ethanolic fronds extract of Asplenium trichomanes L. displayed strongest activity against both cell line with 90.12±0.31a% growth inhibition against HCT 116 and 83±0.31a% against MCF 7 cell line. Extracts of A. trichomanes performed better as compared to A. dalhousiae. Both species appeared to be potent inducers of necrotic cell death.
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Taha Arooj
Department of Botany, Government College University Lahore, Pakistan
Zaheer-ud-din Khan
Department of Chemistry, Government College University Lahore, Pakistan
Sohaib Muhammad
University of Lahore, Teaching Hospital Lahore, Pakistan
Muhammad Ali Raza
Department of Chemistry, Government College University Lahore, Pakistan
Muhammad Umer Farooq Awan
Department of Botany, Government College University Lahore, Pakistan
Zeeshan Sarwer
Department of Botany, Government College University Lahore, Pakistan
Madiha Abdul sattar
University of Lahore, Teaching Hospital Lahore, Pakistan
Nimra Naeem
Department of Microbiology, Government College University Lahore, Pakistan
