The Influence of Moringa Oleifera Leaf Extract on Periodontitis Cases Through Rankl Expression Analysis
Main Article Content
Keywords
Moringa Oleifera, Periodontitis, Porphyromonas gingivalis, RANKL
Abstract
Introduction: Periodontitis is a chronic inflammatory disease that causes damage to the supporting structures of the teeth, and if left untreated this disease will lead to impaired function, appearance, pain and loss of teeth. Periodontitis is caused by bacteria and growing on the tooth surface. The "red complex" bacteria consists of Porphyromonas gingivalis, Treponema denticola, and Tannerella
forsythia. RANKL is a type II homotrimeric transmembrane protein that is expressedas a secretory protein and surrounds the membrane, derived from proteolytic cleavage. RANKL canfunction as an important ligand for the process of osteoclastogenesis.
Objective: to determine the effectiveness of Moringa oleifera leaves in influencing RANKL expression in inflammatory pathways. Research
Methods: The type of research that will be used is a quasi-experimental witha post-test research design with a control group design. This research was conducted in March – November 2022.
Results: On day 0, the average RANKL in the control and treatment groups usingan independent sample t-test showed no significant difference, while on days 7, 14 and 21 the average RANKL in the The control and treatment groups' RANKL scores decreased and the test results showed a significant difference with a p<0.05.
References
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23. Achmad H, Djais AI, Hatta M, Thahir H. Effect of Moringa Leaf Extract (Moringa Oleifera ) on Increasing the Number of Osteoblas as a Marker of Bone Remodeling. Indian Journal of Public Health Research and Development 2019;10(9):1394.
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29. Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop. J. Pharm. Res 2008;7:1019–1024.
30. Davinelli S, Bertoglio JC, Zarrelli A, Pina R, Scapagnini G. A randomized clinical trial evaluating the efficacy of an anthocyanin-maqui berry extract (Delphinol®) on oxidative stress biomarkers. J. Am. Coll. Nutr. 2015;34(Suppl. 1):28–33. doi: 10.1080/07315724.2015.1080108.
31. Murillo AG, Fernandez ML. The relevance of dietary polyphenols in cardiovascular protection. Curr. Pharmacol. Rev. 2017;23:2444–2452. doi: 10.2174/1381612823666170329144307.
Investigative Dentistry. 2010;2:79–91
2. Heitz-Mayfield LJ, Schatzle M, Loe H, Bürgin W, Anerud A, Boysen H, et al. Clinical course of chronic periodontitis. II. Incidence,
characteristics and time of occurrence of the initial periodontal lesion. J Clin Periodontol. 2003:30(10):902–8. DOIs:10.1034/j. 1600051X.
3. Health Research and Development Agency (Balitbangkes). RISKESDAS 2017-2018. Jakarta:Ministry of Health of the Republic of Indonesia. 2018;h207.
4. Eberhard J, Jepsen S, Jervøe-Storm PM, Needleman I, Worthington HV. Full-mouth treatment modalities (within 24 hours) for chronic periodontitis in adults. Cochrane Database System Rev. 2015 Apr 17;(4):CD004622. DOIs:10.1002/14651858. CD004622.
5. Sumintarti, Ramadany S, Handayani H, Achmad H, Mutmainah N, Inayah NH et al. Assessment of the Anti-inflammatory Activities of the Moringa Leaf Extracts in Periodontitis Cases through IL-6 Cytokine Analysis in Wistar (Rattus Copernicus). Macedonian Journal of Medical Sciences. 2022:10(D):124-30.
6. Rafiei M, Kiani F, Sayehmiri F, Sayehmiri K, Sheikhi A, Azodi MZ. Study of Pophyromonas gingivalis in periodontal diseases: A systematic review and meta- analysis. Med J Islamic Republic of Iran. 2017; 31: 62. DOI: 10.18869/mjiri.31.62
7. Mysak J, Podzimek S, Sommerova P, Lyuya-Mi Y, Bartova J, Janatova T, et al. Porphyromonas gingivalis: Major periodontopathic pathogen overview. J Immunol Res. 2014; 2014: 476068. DOI: 10.1155/2014/476068
8. Dwika, Pratama PIW, Oka, Dharmayudha AAG, Sudiamartini, LM, 2016 Identification of Chemical Compounds of Moringa Leaf Ethanol Extract (Moringa oleifera L) in Bali, Indonesia Medicus Veterinus, October 2016, p. 466.
9. Aminah, Syarifah., Tezar, Ramdhan & Muflihani Yanis. Nutritional Contentand Functional Properties of Moringa (Moringa oleifera) Scientific Article Research Results from the Jakarta Agricultural Technology Study Center. 2015;36.
10. Rieuwpassa IE, Ramadany S, Sumintarti, Israyani, Achmad H, et al. Journal of International Dental and Medical Research;
Diyarbakir. 2022;15(2):611-617.
11. Mescher, AL. Junqueira's Basic Histology 13ͭ ͪEdition. 2013. United States: McGraw Hill Education.
12. Tang JH, H Meng, Y Zhao, et al. Downregulation of RANKL and RANKL/Osteoprotegrin Ratio in Human Periodontal Ligament Cells During theirOsteogenic Differentiation. Journal of Periodontal Research. 2016;51: 125-132.
13. Wisdom, N. et al. The role of Rankl in the process of alveolar bone resorption in diabetic conditions'. 2013;10(3):105–109.
14. Achmad H, Supriatno, Singgih MF, Hendrastuti H. Akt Signal Transduction Pathways and Nuclear Factor-kappa B (NF-κB) Transcription as a Molecular Target of Oral Tongue Squamous Cell Carcinoma (SP-C1) Using Papua's Anthill Plant (Myrmecodia pendans). Pak J Biol Sci. 2016;19(8-9):323-330.
15. Grimaud, et al. Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis. National library of Medicine. 2013;163(5):2021-31.
16. Fard MT, Arulselvan P, Karthivashan G, Adam SK, Fakurazi S. Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages. Pharmacogn Mag. 2015 Oct; 11(Suppl 4): 556–S563.
17. Owen J, Punt J, Stranford S. Kuby Immunology, 7th ed. Freeman, W. H. & Company ;2013.
18. Omodanisi E, Aboua Y, Oguntibeju O. Assessment of the Anti-Hyperglycaemic, AntiInflammatory and Antioxidant Activities of the Methanol Extract of Moringa Oleifera in Diabetes-Induced Nephrotoxic Male Wistar Rats. Molecules. 2017; 22(4): 439.
19. Tobeiha M, Moghadasian MH, Amin N, Jafarnejad S. RANKL/RANK/OPG Pathway: A Mechanism Involved in Exercise-Induced Bone Remodeling. BioMed Research International. 2020;2020: 1–11. doi:10.1155/2020/6910312
20. J-Y Kim, H-J Kim, D-H Park, Y-A Shin, S-K Min, C-S Kim. Effects of acute exercise on RANKL/RANK/ OPG pathway and bonemetabolic markers in healthy college female. Exercise Science. 2018;27(3):225–231.
21. Tanaka T, Narazaki M, Kishimoto T. IL-6 in Inflammation, Immunity, and Disease. Cold Spring Harbor Perspectives in Biology.2014; 6(10): a016295–a016295.
22. Liuzzi JP, Lichten LA, Rivera S, Blanchard RK, Aydemir TB, Knutson MD, Cousins RJ. Interleukin-6 regulates the zinc transporter Zip14
in liver and contributes to the hypozincemia of the acute-phase response. Proceedings of the National Academy of Sciences. 2005; 102(19) :6843–6848.
23. Achmad H, Djais AI, Hatta M, Thahir H. Effect of Moringa Leaf Extract (Moringa Oleifera ) on Increasing the Number of Osteoblas as a Marker of Bone Remodeling. Indian Journal of Public Health Research and Development 2019;10(9):1394.
24. Newman M, Takei H, Klokkevold P, Carranza F. Newman and Carranza’s clinical periodontology. 13th ed. Philadelphia: Elsevier Saunders; 2018. p. 346–7.
25. Quamilla N. Stres dan kejadian periodontitis (kajian literatur). J Syiah Kuala Dent Soc 2016; 1(2): 161–8.
26. Putri CF, Bachtiar EW. Porphyromonas gingivalis and pathogenesis of cognitive disfunction: role of neuroinf lammation
cytokine (Literature review). Cakradonya Dent J 2020; 12(1): 15–23.
27. Jamal M, Tasneem U, Hussain T, Andleeb S. Bacterial biofilm: Its composition, formation and role in human infections. Res Rev J Microbiol Biotechnol 2015; 4(3): 1–14.
28. Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm. Virulence. 2011; 2(5): 435–44.
29. Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop. J. Pharm. Res 2008;7:1019–1024.
30. Davinelli S, Bertoglio JC, Zarrelli A, Pina R, Scapagnini G. A randomized clinical trial evaluating the efficacy of an anthocyanin-maqui berry extract (Delphinol®) on oxidative stress biomarkers. J. Am. Coll. Nutr. 2015;34(Suppl. 1):28–33. doi: 10.1080/07315724.2015.1080108.
31. Murillo AG, Fernandez ML. The relevance of dietary polyphenols in cardiovascular protection. Curr. Pharmacol. Rev. 2017;23:2444–2452. doi: 10.2174/1381612823666170329144307.
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Published
Feb 23, 2023
Article Details
How to Cite
Irene Edith Rieuwpassa, Surijana Mappangara, Israyani, Harun Achmad, Harmawaty, & Arrang Sesioria. (2023). The Influence of Moringa Oleifera Leaf Extract on Periodontitis Cases Through Rankl Expression Analysis. Journal of Population Therapeutics and Clinical Pharmacology, 30(1), 355–362. https://doi.org/10.47750/jptcp.2023.1094
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