Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway for periodontal tissue regeneration in rats

Main Article Content

Sumintarti Sugiharto
Sri Ramadany
Hendrastuti Handayani
Harun Achmad
Asdar Gani
Maria Tanumihardja
Arrang Sesioria
Harmawaty

Keywords

ALP, Hypercolestrolemia ,Antioxadinat

Abstract

Periodontitis is a chronic inflammatory disease that causes damage to the supporting structures of the teeth, and if left untreated this disease will lead to impaired function, appearance, pain, and loss of teeth. Periodontitis is caused by bacteria adhering to and growing on the tooth surface and will trigger an inflammatory response in the body. NF-κB has a very important role in the immune system. NFκB can control transcription, cytokines, antimicrobial effectors, and genes that regulate cell differentiation, cell survival, and proliferation so that they can regulate various aspects of innate and adaptive immune responses. Therefore, down-regulating the NF-κB signaling pathway is one way to reduce chronic inflammation. Moringa leaf extract as a natural ingredient for anti-inflammatory
adjunctive therapy can be an alternative in the treatment of periodontitis.
Objective. This study aims to determine the effectiveness of Moringa oleifera leaves in influencing NF-κB expression in the pathway of inflammatory pathways.
Methods. Experimental laboratory research and clinical trials with posttest-only control group design. Twenty- four Wistar rats were divided into two groups. Then the periodontitis was made with an injection of Porphyromonas gingivalis bacteria on the mandible, the control group was given aquades and the treatment group was given a Moringa oleifera gel extract. Wistar rats were sacrificed on days 0,7, 14, and 21, and the mandible bone was then taken for immunohistochemical analysis to determine the levels of NF-κB.
Result. On days 7, 14, and 21, the expression levels of NF-κB were significantly different between the two groups.The group added with Moringa oleifera leaf extract showed a faster decrease in NFκB expression than the control group.
Conclusion. Moringa oilefera extract can inhibit NF-κB expression in inflammatory pathways.

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