Circulating microRNA-92a as biomarkers for primary woman breast cancer Iraq population

Main Article Content

Noora Wael Rasheed

Keywords

HDL, TG, TC, VLDL, miRNA, and pri-miRNA

Abstract

MiRNA genes have their own the beginning and end of RNA transcripts and may be intragenic or intergenic. When the host genes' intron sequences match exactly those of the splice-site-containing pre-miRNA, mirtons are created. Processing of Drosha is not necessary for the development of Merton, and they can be generated without it. This study included 75 patients suffering from breast
cancer based on the oncologist confirmation and 50 healthy subjects. Using Friedwald's method, LDL cholesterol may be quantitatively determined from total cholesterol, triglycerides, and the concentration of HDL cholesterol. A The total RNA was extracted from blood samples using a readyto-use reagent (TRI Reagent®; ZYMO RESEARCH; USA). Promega's QuantusTM Fluorometer (USA)and PromegaQuantiFluor® Dye Systems were used for the analysis. Total RNA, quantitative MIR29a gene expression was estimated by real time PCR in patient and control group. The mean level of BMI showed none-significant difference between the patients and control. High Density Lipid (HDL) levels showed a significant higher mean in patients than in control. The MIR29a gene expression levels of patients showed a significantly higher level than those of control. Levels of cancer marker Ca125 showed high levels in patients compared to control.

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