Antioxidant activity of silver nanoparticles using Picrorhiza Kurroa root extract
Main Article Content
Keywords
Antioxidant, silver nanoparticles, Picrorizha, Assays, Free - radicals
Abstract
Introduction: Picrorizha Kurroa, also known as "KUTUKI," is a small perennial herb in the scrophulariaceae family. The rhizome of Picrorizha has traditionally been used to treat worms, constipation, fever, and liver ailments. Iridoid glycoside, picroside LIl,III, and kutikoside are the most important active constituents of Picrorizha. Silver and its compounds, including silver nanoparticles, have antimicrobial properties. According to the findings of the silver nanoparticles study, antibacterial agents inhibit resistance strains through a variety of mechanisms, including actions involving the induction of oxidative stress and interactions with enzymes and proteins. The study's goal is to determine the antioxidant activity of silver nanoparticles synthesised with Picrorhiza Kurroa root extract.
Materials And Methods: Firstly wash the conical flask and 1000 grams of Picrorizha is taken. 100 ml of distilled water was added. Add AgNO3 solution, mark six centrifugation tubes and the extract was filled with 14mleach. Results were analysed using the UV visible spectrometer and assays ( DPPH, H2O2 ).
Results: There was a dose dependent increase in the zone of inhibition of DPPH and H202 assay from CONC. ranging from 10- 50 ug/ml.
Conclusion: This study, shows that the ethanol extracts of Picrorizha exhibits wide range of antioxidant activity against ROS and oxidative damage. The Antioxidant activity was determined using DPPH assay and H202 assay.
References
2. Sies H. Oxidative stress: Oxidants and antioxidants. Exp Physiol 2020;82(2):291-5
3. Pai A, K.CS. 2021. “ In vitro antidiabetic activity of methanolic extract of leaves and fruits of Pouteria campechiana (Kunth) Baehni.VOL. 41 NO. 4 (2021): BIOMEDICINE: 2021; 41(4)
4. Sanjay S,2021. “The Study Of Potentiality Of Picrorhiza Kurroa Root Proteins To Inhibit Free Radicals And Α-Amylase Enzyme”.
5. Ames B.N, Shigenega M.K, Hagen T.M, “Oxidants and the degenerative diseases of ageing” Proc Nati Acad Sci , 90: 7915 – 22, 1993
6. Shenoy R, Shirwaikar A, “Anti-inflammatory and free radical scavenging studies of Hyptis suaveolens (labiatae)” Indian drugs: 39, 574 –577, 2003.
7. Patil S Jolly CI, Narayanan S, free radical scavenging activity of acacia catechu and Rotula aquatica: implications in cancer therapy, Indian drugs: 40, 328 – 332, 2005.
8. Evans P, Halliwall B, “Free radicals and hearing” Ann N Y Acad Sci, 884: 19, 2016.
9. Devasagayam T.P.A, Kesavan P.C, “radioprotective and antioxidant action of caffeine : mechanistic considerations, Indj exp boil; 41, 267 – 269, 1996.
10. A.V.Badarinath,2010. “A Review on In-vitro Antioxidant Methods: Comparisions, Correlations and Considerations”.
11. Rai, M.; Yadav, A.; Gade, A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv. 2009, 27, 76–83. [CrossRef] [PubMed]
12. Pandit, R. Green synthesis of silver nanoparticles from seed extract of Brassica nigra and its antibacterial activity. Nusant. Biosci. 2015, 7, 15–19. [CrossRef]
13. Abbasi, E.; Milani, M.; Fekri Aval, S.; Kouhi, M.; Akbarzadeh, A.; Tayefi Nasrabadi, H.; Nikasa, P.; Joo, S.W.; Hanifehpour, Y.; Nejati-Koshki, K.; et al. Silver nanoparticles: Synthesis methods, bioapplications and properties. Crit. Rev. Microbiol. 2016, 42, 1–8. [CrossRef] [PubMed]
14. Jiang, H.; Manolache, S.; Wong, A.C.L.; Denes, F.S. Plasma-enhanced deposition of silver nanoparticles onto polymer and metal surfaces for the generation of antimicrobial characteristics. J. Appl. Polym. Sci. 2008, 93, 1411–1422. [CrossRef]
15. Hwang, I.; Hwang, J.H.; Choi, H.; Kim, K.-J.; Lee, D.G. Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved. J. Med. Microbiol. 2012, 61, 1719–1726.
16. Amit Kumar Mittal, 2012. “Free radicals scavenging and antioxidant activity of AgNPs synthesis from flower extract of Rhododendron dawiicum.
17. Zdenka Bedlovicova, 2020. “A brief overview on antioxidant activity determination of silver nanoparticles.
18. Aldhuwayhi, Sami, Sreekanth Kumar Mallineni, Srinivasulu Sakhamuri, Amar Ashok
19. Thakare, Sahana Mallineni, Rishitha Sajja, Mallika Sethi, Venkatesh Nettam, and Azher Mohiuddin Mohammad. 2021. “Covid-19 Knowledge and Perceptions Among Dental
20. Specialists: A Cross-Sectional Online Questionnaire Survey.” Risk Management and
21. Healthcare Policy 14 (July): 2851–61.
22. Dua, Kamal, Ridhima Wadhwa, Gautam Singhvi, Vamshikrishna Rapalli, Shakti Dhar Shukla, Madhur D. Shastri, Gaurav Gupta, et al. 2019. “The Potential of siRNA Based Drug Delivery in Respiratory Disorders: Recent Advances and Progress.” Drug Development Research 80 (6): 714–30.
23. Gan, Hongyun, Yaqing Zhang, Qingyun Zhou, Lierui Zheng, Xiaofeng Xie, Vishnu Priya Veeraraghavan, and Surapaneni Krishna Mohan. 2019. “Zingerone Induced Caspase-Dependent Apoptosis in MCF-7 Cells and Prevents 7,12-Dimethylbenz(a)anthracene-Induced Mammary
Carcinogenesis in Experimental Rats.” Journal of Biochemical and Molecular Toxicology 33 (10): e22387.
24. Jayaraj, Gifrina, Pratibha Ramani, Herald J. Sherlin, Priya Premkumar, and N. Anuja. 2015. “Inter-Observer Agreement in Grading Oral Epithelial Dysplasia – A Systematic Review.” Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. https://doi.org/10.1016/j.ajoms.2014.01.006.
25. Li, Zhenjiang, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Srinivasa Rao Bolla, Hariprasath Lakshmanan, Subramanian Kumaran, Wilson Aruni, et al. 2020. “Apoptotic Induction and Anti-Metastatic Activity of Eugenol Encapsulated Chitosan Nanopolymer on Rat Glioma C6 Cells via Alleviating the MMP Signaling Pathway.” Journal of Photochemistry and Photobiology B: Biology. https://doi.org/10.1016/j.jphotobiol.2019.111773
26. Markov, Alexander, Lakshmi Thangavelu, Surendar Aravindhan, Angelina Olegovna Zekiy, Mostafa Jarahian, Max Stanley Chartrand,
Yashwant Pathak, Faroogh Marofi, Somayeh Shamlou, and Ali Hassanzadeh. 2021. “Mesenchymal Stem/stromal Cells as a Valuable Source for the Treatment of Immune-Mediated Disorders.” Stem Cell Research & Therapy 12 (1): 192.
27. Mohan, Meenakshi, and Nithya Jagannathan. 2014. “Oral Field Cancerization: An Update on Current Concepts.” Oncology Reviews 8 (1): 244.
28. Neelakantan, Prasanna, Deeksha Grotra, and Subash Sharma. 2013. “Retreatability of 2 Mineral Trioxide Aggregate-Based Root Canal
Sealers: A Cone-Beam Computed Tomography Analysis.” Journal of Endodontia 39 (7): 893–96.
29. Paramasivam, Arumugam, Jayaseelan Vijayashree Priyadharsini, Subramanian Raghunandhakumar, and Perumal Elumalai. 2020. “A Novel COVID-19 and Its Effects on Cardiovascular Disease.” Hypertension Research: Official Journal of the Japanese Society of Hypertension.
30. Sheriff, K. Ahmed Hilal, K. Ahmed Hilal Sheriff, and Archana Santhanam. 2018. “Knowledge and Awareness towards Oral Biopsy among Students of Saveetha Dental College.” Research Journal of Pharmacy and Technology. https://doi.org/10.5958/0974-360x.2018.00101.4.
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