EFFECT OF STABILITY, BIO-BURDEN, KILLING TIME, PRESERVATIVE ASSESSMENT, AND GC-ANALYSIS ON NOVEL DEVELOPED HERBISOL - A NEW MOUTH WASH
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Keywords
Peridontal infection, Dental solution, Bioburden, Preservative efficacy, Essential oils, Gas Chromatography
Abstract
In Pakistan, individuals with oral issues are consistently increasing with an alarming rate and cases are routinely reported to dental practioners. Oral cleanliness is vital to get freed from plaque and gum disease which is possible by utilization of various mouthwashes in which certain fundamental oil having antimicrobial and sedative properties are incorporated.A few organic compounds are known to present in various oils of plants. This research was conducted to evaluate the stability, bioburden, Antimicrobial potentials, time measurement, and preservative tests to determine antimicrobial impacts and assessment of the movement of mouthwashes against pathogens included in dental caries and oral diseases. GC analysis was also observed to quantify herbal constituents present in the newly formulated mouth wash. Mouth wash Herbisol was separately extracted with N-hexane. Our results showed that herbal mouth wash were effective against different oral pathogens as compared to herbal dental rinse solution. Sodium benzoate is used as a preservative effective against E.coli, S.aureus&A. brazilensis. The recovery was 99% - 96.6% for menthol, 96.8% for eugenol, and 91.4% for eucalyptol which are ingredients of herbisol. GC analysis affirmed that herbisol are a naturally dynamic mouth wash showing that a specific quantity of herbal actives attained in the mouth wash. our results indicated that using herbisol mouth wash twice a day, will maintain oral hygiene from microorganisms. Herbisol maintains stability level in all parameters. Any bio-burden and specified pathogens were not seen.
References
2. Attoub, S., Karam, S. M., Nemmar, A., Arafat, K., John, A., Al-Dhaheri, W., ... & Raza, H. (2014). Short-term effects of oral administration of Pistacia lentiscus oil on tissue-specific toxicity and drug metabolizing enzymes in mice. Cellular Physiology and Biochemistry, 33(5), 1400-1410.
3. Bhavna, J. K., & Vidhya, D. (2012). Herbal mouthwash-A gift of nature. Int J Pharma and Bio Sci, 3(2), 47-52.
4. Bozorgi, M., Memariani, Z., Mobli, M., Salehi Surmaghi, M. H., Shams-Ardekani, M. R., & Rahimi, R. (2013). Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology. The Scientific World Journal, 2013.
5. Brown, E. D., & Wright, G. D. (2016). Antibacterial drug discovery in the resistance era. Nature, 529(7586), 336-343.
6. Cortelli, S. C., Cortelli, J. R., Shang, H., Costa, R., & Charles, C. A. (2014). Gingival health benefits of essential-oil and cetylpyridinium chloride mouthrinses: a 6-month randomized clinical study. Am J Dent, 27(3), 119-26.
7. Costa, R., Ragusa, S., Russo, M., Certo, G., Franchina, F. A., Zanotto, A., ... & Germanò, M. P. (2016). Phytochemical screening of Artemisia arborescens L. by means of advanced chromatographic techniques for identification of health-promoting compounds. Journal of pharmaceutical and biomedical analysis, 117, 499-509.
8. Dua, K., Sheshala, R., Al-Waeli, H. A., Gupta, G., & Chellappan, D. K. (2015). Antimicrobial efficacy of extemporaneously prepared herbal mouth-washes. Recent Pat Drug Deliv Formul, 9(3), 257-261.
9. Eslami, N., Ahrari, F., Rajabi, O., & Zamani, R. (2015). The staining effect of different mouthwashes containing nanoparticles on dental enamel. Journal of clinical and experimental dentistry, 7(4), e457.
10. Filippi, J. J., Belhassen, E., Baldovini, N., Brevard, H., & Meierhenrich, U. J. (2013). Qualitative and quantitative analysis of vetiver essential oils by comprehensive two-dimensional gas chromatography and comprehensive two-dimensional gas chromatography/mass spectrometry. Journal of Chromatography A, 1288, 127-148.
11. Janta, P., Kulsing, C., & Nhujak, T. (2018). Characterization of Volatile Compounds in Tom Yum Soup by Headspace-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry Combined with Sensory Evaluation Techniques.
12. Janta, P., Pinyo, D., Yodta, Y., Vasasiri, P., Weidenbach, M., Pursch, M., ... & Kulsing, C. (2021). Strategies towards simpler configuration and higher peak capacity with comprehensive multidimensional gas chromatography. RSC Advances, 11(14), 7946-7953.
13. Jayanti, I., Jalaluddin, M., Avijeeta, A., Ramanna, P. K., Rai, P. M., & Nair, R. A. (2018). In vitro Antimicrobial Activity of Ocimum sanctum (Tulsi) Extract on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. The journal of contemporary dental practice, 19(4), 415-419.
14. Khan, M. F., Tang, H., Lyles, J. T., Pineau, R., Mashwani, Z. U. R., & Quave, C. L. (2018). Antibacterial properties of medicinal plants from Pakistan against multidrug-resistant ESKAPE pathogens. Frontiers in pharmacology, 9, 815.
15. Kirkin, C., Mitrevski, B., Gunes, G., & Marriott, P. J. (2014). Essential‐oil analysis of irradiated spices by using comprehensive two‐dimensional gas chromatography. ChemPlusChem, 79(6), 798-803.
16. Lebanov, L., Tedone, L., Kaykhaii, M., Linford, M. R., & Paull, B. (2019). Multidimensional gas chromatography in essential oil analysis. Part 2: Application to characterisation and identification. Chromatographia, 82(1), 399-414.
17. Mustafa, M. W., Ungphaiboon, S., Phadoongsombut, N., Pangsomboon, K., Chelae, S., & Mahattanadul, S. (2019). Effectiveness of an alcohol-free chitosan–curcuminoid mouthwash compared with chlorhexidine mouthwash in denture stomatitis treatment: a randomized trial. The journal of alternative and complementary medicine, 25(5), 552-558.
18. Nasim, by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry. Natural product research, 31(7), 853-856.
19. Sciarrone, D., Pantò, S., Ragonese, C., Tranchida, P. Q., Dugo, P., & Mondello, L. (2012). Increasing the isolated quantities and purities of volatile compounds by using a triple deans-switch multidimensional preparative gas chromatographic system with an apolar-wax-ionic liquid stationary-phase combination. Analytical chemistry, 84(16), 7092-7098.
20. Seeley, J. V. (2012). Recent advances in flow-controlled multidimensional gas chromatography. Journal of Chromatography A, 1255, 24-37.
21. Silva, L. N., Zimmer, K. R., Macedo, A. J., & Trentin, D. S. (2016). Plant natural products targeting bacterial virulence factors. Chemical Reviews, 116(16), 9162-9236.
22. Sukkarwalla, A., Ali, S. M., Lundberg, P., & Tanwir, F. (2013). Efficacy of miswak on oral pathogens. Dental research journal, 10(3), 314.
23. Tong, H., Liu, J., Yao, X., Jia, H., Wei, J., Shao, D., ... & Li, B. (2018). High carriage rate of mcr-1 and antimicrobial resistance profiles of mcr-1-positive Escherichia coli isolates in swine faecal samples collected from eighteen provinces in China. Veterinary microbiology, 225, 53-57.
24. Tranchida, P. Q., Sciarrone, D., Dugo, P., & Mondello, L. (2012). Heart-cutting multidimensional gas chromatography: a review of recent evolution, applications, and future prospects. Analytica chimica acta, 716, 66-75.
25. Vlachojannis, C., Chrubasik‐Hausmann, S., Hellwig, E., & Al‐Ahmad, A. (2015). A preliminary investigation on the antimicrobial activity of Listerine®, its components, and of mixtures thereof. Phytotherapy Research, 29(10), 1590-1594.
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Mariyah Yacoob Bawa
Head of Quality Operations & R&D, Zaiqa Food Industries, University of Karachi, Pakistan
Afsheen Aqeel
Teaching Assistant, Department of Microbiology, University of Karachi, Pakistan
Muffrah Hasan
Department of Microbiology, University of Karachi, Pakistan
Saima Saleem
Associate Professor, Department of Pharmacy Practice, Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan.
Ambreen Rafique
Clinical Laboratory Sciences, Dow Institute of Medical Technology, Dow University of Health Sciences, Karachi, Pakistan
Yasmin Khanam
Assistant Director, Pakistan Standards and Quality Control Authority
Munir Ahmed Khan
Assistant Director, Pakistan Standards and Quality Control Authority