A QUANTITATIVE ANALYSIS ON HUMAN MICROVASCULAR LYMPHATIC AND BLOOD ENDOTHELIAL CELLS PRODUCE FIBRILLIN
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Keywords
Immunohistochemistry; lymphatic vessels; microfibril-associated glycoprotein-1; microvascular endothelial cells
Abstract
Fibrillin microfibrils serve as the anchoring filaments that join the lymphatic endothelium to the surrounding elastic fibers and provide a scaffold for the deposition of elastin in the arterial wall. Previous researches confirmed that thoracic duct endothelial cells produce an uneven web, while bovine arterial endothelial cells deposit fibrillin in a honeycomb pattern together with microfibril-associated glycoprotein (MAGP)-1. By sequentially using the pan-endothelial marker CD31 and the lymphatic-specific marker D2-40, the current immunohistochemistry investigation aimed to confirm whether lymphatic and blood human dermal microvascular endothelial cells (HDMECs) isolated from human foreskin deposit fibrillin and MAGP-1. Both fibrillin and MAGP-1 co-localized in both cell types and were deposited in the same culture in varying patterns of increasing complexity. Wide-mesh honeycomb was created by fibrillin microfibrils, leaving gaps that were subsequently filled by fibrillin. Blood and lymphatic HDMECs exhibited a fibrillin deposition mechanism that was essentially identical to that of bovine large artery endothelial cells. Fibrillin was first deposited in some lymphatic HDMECs as evenly distributed short fibrillin strands, most likely due to anchoring filaments that were carried over from the original capillaries. Our results demonstrate the involvement of lymphatic and blood endothelial cells in the deposition of fibrillin in human skin.
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Dr Suresh Kumari
Associate professor, Department of Anatomy, Al Falah School of Medical Sciences and Research Center Dhauj Faridabad