EVALUATION OF THE SAFETY PROFILE OF BANANA PEEL EXTRACT IN RATS
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Keywords
Banana peel extract, toxicity, hematology, biochemical parameters, physiological parameters
Abstract
The current study assessed the effect of supercritical fluid extract of the banana peel (an agricultural waste) on some haematological and biochemical parameters in Sprague Dawley rats. Twenty (20) rats were assigned as 4 groups and each group had 5 rats. Group 1 was assigned as control. Groups 2, 3 and 4 were administered 400.00, 800.00 and 1200.00 mg/kg per body weight respectively of the banana peel extract for a period of 21 days. Blood samples were collected by cardiac puncture and analyzed. Hemoglobin concentration, PCV, WBC, Lymphocyte, Monocyte values and total RBC count showed significant difference between treatment groups. But these changes did not cause any toxicity in the rats as their variation was in range when compared with the control group. These changes were noted due to possible genetic variations in the rats. Total Platelet count, MCV, MCH and MCHC, Eosinophils, and Neutrophils did not show any significant differences between the treatment groups. No changes were observed in rats’ weight and feed intake having banana peel extract in their diets. This confirms the neutral behavior of the extracts in the rats thus not causing any toxicity. The extract is saved to be used in preparation of any functional food in the future. Moreover, extract can be used as preservative in stabilization of the food products in the replacement of the synthetic preservatives. These supposed uses will be covered and discussed in upcoming research objectives.
References
2. Falya, Y., S.A. Sumiwi and J. Levita. 2020. Mini Review: Toxicity study of plant extracts. IOSR Journal of Pharmacy and Biological Sciences. 15(2): 25-32.
3. FAOSTAT. 2020. Production quantity of banana. 2018. Food and Agriculture Organisation of the United Nations.
4. Gomes, S., B. Vieira, C. Barbosa and R. Pinheiro. 2022. Evaluation of mature banana peel flour on physical, chemical, and texture properties of a gluten‐free Rissol. Journal of Food Processing and Preservation. 46(8): 14441.
5. Haddad K. H., M. Taheri, Z. Qotbi, A. Zahedi, F. Izadpanah and H. Nikzad. 2024. Protective effect of combined ginger and pumpkin seed extracts on cyclophosphamide-induced hepatotoxicity in rats. Advances in Traditional Medicine. 24(2): 583-591.
6. Halim, S.Z., N.R. Abdullah, A. Afzan, B.A. Rashid, I. Jantan and Z. Ismail. 2011. Acute toxicity study of Carica papaya leaf extract in Sprague Dawley rats. Journal of Medicinal Plants Research. 5(10):1867-72.
7. Islam, M.R., M.M. Kamal, M.R. Kabir, M.M. Hasan, A.R. Haque and S.K. Hasan. 2023. Phenolic compounds and antioxidants activity of banana peel extracts: Testing and optimization of enzyme-assisted conditions. Measurement: Food. 10: 100085.
8. Kumar, P., A.K. Verma, P. Umaraw, N. Mehta and O.P. Malav. 2020. Plant phenolics as natural preservatives in food system. In Plant Phenolics in Sustainable Agriculture: 367-406.
9. Majesty, D., U. Amadike and A. Benjamin. 2013. Effect of Solanum Macrocarpon fruit on Haematology, hepatic and renal function. Advances in Biochemistry. 1(2): 28-32.
10. Singh, N., V. Kamath, K. Narasimhamurthy and P.S. Rajini. 2008. Protective effect of potato peel extract against carbon tetrachloride-induced liver injury in rats.
11. Nisha, P. and S. Mini. 2014. In vitro antioxidant and antiglycation properties of methanol extract and its different solvent fractions of Musa paradisiaca L.(Cv. Nendran) inflorescence. International Journal of Food Properties. 17(2): 399-409.
12. Uddin, N., M.R. Hasan, M.M. Hasan, M.M. Hossain, M.R. Alam, M.R. Hasan, A.F.M.M. Islam, T. Rahman and M.S. Rana. 2014. Assessment of toxic effects of the methanol extract of Citrus macroptera Montr. fruit via biochemical and hematological evaluation in female Sprague-Dawley rats.
13. Ojuade, F.I., R.O. Ayanniyi, H.A. Olumoh-Abdul, A.R. Ayeni and M.W. Rasheed. 2021. Acute and subacute toxicity of aqueous extract of Allium cepa peels in wistar albino rats: determination of acute and subacute toxicity in wistar albino rats. Iranian Journal of Pharmaceutical Sciences. 17(2): 51-66.
14. Pereira, A. and M. Maraschin. 2015. Banana (Musa spp) from peel to pulp: ethnopharmacology, source of bioactive compounds and its relevance for human health. Journal of Ethnopharmacology. 160: 149-163.
15. Phipps, K.R., D. Lozon and N. Baldwin. 2021. Genotoxicity and subchronic toxicity studies of supercritical carbon dioxide and acetone extracts of rosemary. Regulatory Toxicology and Pharmacology. 119:104826.
16. Santos, P.H., J.C. Kammers, A.P. Silva, J.V. Oliveira, and H. Hence. 2021. Antioxidant and antibacterial compounds from feijoa leaf extracts obtained by pressurized liquid extraction and supercritical fluid extraction. Food Chemistry. 344:128620.
17. Sidhu, J. S. and T.A. Zafar. 2018. Bioactive compounds in banana fruits and their health benefits. Food Quality and Safety. 2(4): 183-188.
18. Tcheutchoua, Y.C., D.C. Bilanda, Y.S.M. Ngadena, P.D.D. Dzeufiet, P.E. Owona, R.B.A. Goufani, R.N. Fifen, L.M. Nguegan, M. Noubom, T. Dimo and P. Kamtchouing. 2022. Acute and subchronic toxicity studies on the aqueous extract of the plant mixture (Bidens pilosa and Cymbopogon citratus aerial parts) in rat model. Journal of Toxicology. 2022(1): 1998433.
19. Wolff, F. R., M.F. Broering, J.D. Jurcevic, T. Zermiani, A. Bramorski, J.D.C. Vitorino, A. Malheiros and J. R. Santin. 2019. Safety assessment of Piper cernuum Vell. (Piperaceae) Leaves extract Acute, sub-acute toxicity, and genotoxicity studies'. Journal of Ethnopharmacology. Elsevier Ireland Ltd. 230: 109–116.
20. Zaini, H. M., J. Roslan, S. Saallah, E. Munsu, N.S. Sulaiman and W. Pindi. 2022. Banana peels as a bioactive ingredient and its potential application in the food industry. Journal of Functional Foods. 92.
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Sidra-tul-Muntaha
Department of Food Science and Technology, Government College Women University, Faisalabad. Pakistan
Saima Tehseen
Department of Food Science and Technology, Government College Women University, Faisalabad. Pakistan
Mahwash Aziz
Department of Food Science and Technology, Government College Women University, Faisalabad. Pakistan
Zill-i-Huma Nazli
Department of Chemistry, Government College Women University, Faisalabad. Pakistan