EVALUATION OF PHENOTYPIC METHODS OF BETA-LACTAMASE PRODUCING METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN A TERTIARY CARE HOSPITAL
Main Article Content
Keywords
β-lactamase, S. aureus, MRSA, Cloverleaf test, Masuda double disc test
Abstract
ABSTRACT
Background: Methicillin-Resistant Staphylococcus aureus (MRSA) is one of the most common causes of nosocomial and community infections, and the majority of clinical isolates are β-lactam and multidrug-resistant.
Resistance to β-lactam antibiotics is frequently caused by the development of penicillin-binding proteins (PBPs) and β-lactamases. The identification of β-lactamases is critical for selecting the most effective antibiotic treatment.
Objective: To compare various phenotypic methods for the detection of β-lactamase producing Methicillin-Resistant Staphylococcus aureus (MRSA) in a Tertiary Care Hospital.
Material and Methods: A Prospective Hospital study conducted from January 2023 to December 2023 from various clinical specimens that were sent for processing in the Department of Microbiology, United Institute of Medical Sciences, Prayagraj, from patients attending various OPDs and admitted in IPDs.
A total of 1877 patients’ sample, received from various departments were processed. All MRSA isolates were tested for β-lactamase production by using various phenotypic methods like cloverleaf test, Masuda double-disc test, Double disc method and Penicillin disc diffusion method.
Result: Characterization of β-lactamases is important for choosing appropriate antibiotic therapy; therefore 97S. aureus were isolated with 49 MRSA and 48 MSSA. The findings of this study revealed that the Cloverleaf test and Masuda test had high accuracy (100%) compared with other methods tested for the detection of MRSA β-lactamase production.
Conclusion: The study shows that the Cloverleaf and Masuda double-disc were more accurate and superior methods in the detection of β-lactamase producing S. aureus and suggests that these methods can be done as routine tests in the Microbiology Laboratory.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Nandlal Kumar
Assistant Professor, Department of Microbiology, United Institute of Medical Sciences, Prayagraj, India.
Pratima Kumari
Tutor, Department of Microbiology, United Institute of Medical Sciences, Prayagraj, India.
Saumya Singh
Professor, Department of Microbiology, United Institute of Medical Sciences, Prayagraj, India.
Devesh Sharma
Professor, Department of Microbiology, United Institute of Medical Sciences, Prayagraj, India.
Rajan Pathak
Senior Resident, Department of Microbiology, United Institute of Medical Sciences, Prayagraj, India.