INSIGHTS FROM CRISPR-CAS TECHNOLOGY: REVOLUTIONIZING CANCER RESEARCH, UNVEILING THERAPEUTIC STRATEGIES, AND ADVANCING PERSONALIZED CANCER TREATMENT APPROACHES
Main Article Content
Keywords
CRISPR-Cas, cancer research, therapeutic strategies, personalized medicine, gene editing, cancer types.
Abstract
CRISPR-Cas system has revolutionized cancer research by the possibility to carry out gene manipulations and new therapies. This work attempts to assess the impact of CRISPR-Cas systems across different cancers by conducting a literature search; among the 400 articles, 204 articles used the CRISPR-Cas technology. The literature was reviewed based on its use of CRISPR-Cas for functional genomics, therapy, and patient-specific treatment. Data mining concentrated on the experimental design, results concerning the specific type of cancer, treatment effectiveness, and advancements in individualized treatment. When it comes to breast cancer, the meta-analysis established that CRISPR-Cas has helped to define critical oncogenes and tumor suppressor genes which in-turn has enriched the knowledge base on tumor biology as well as the mechanisms of resistance. In lung cancer the targeted gene editing has led to the emergence of new drug targets. In the case of prostate cancer, CRISPR Cas has made it easier to develop gene knock out models which has helped in analyzing androgen receptor signaling and drug resistance. In colorectal cancer, CRISPR-Cas is used for the analysis of mutation profile and signaling pathways linked to carcinogenesis. CRISPR-Cas studies on ovarian cancer specifically on the BRCA mutations and how to overcome resistance to drugs. There are trend reports of accessing pancreatic cancer models to analyze genetic changes and to develop new treatments using CRISPR-Cas. Melanoma research has progressed from using the CRISPR-Cas system to discover mutation-driven paths and create immunotherapies. In head and neck cancer such as TP 53 and PIK3CA mutations; knowledge about tumor microenvironments as well as immune evasion. Thus, knowledge of alterations such as CTNNB1 in liver cancer has instituted the construction of liver cancer models and improvements in drug screening with gene therapy. The sphere of leukemia has been benefited from CRISPR-Cas system in understanding the genetic mutations and for the design of gene-editing therapy. Lastly, Lymphoma investigations have used CRISPR-Cas to reveal actual aspects of molecular biology as well as develop new treatments. Researches based on CRISPR-Cas systems have improved the knowledge of cancer genetics and characteristics. Still, issues including off-target effects and the delivery methods hold onto their importance, and thus, there is a need to continue with the research in the application of the CRISPR-Cas in oncology.
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Aug 7, 2024
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Zainab Kalsoom, Warda Javed, Sarshar Sannam, Muqadas Fatima, Aqsa Manzoor, Durga Devi, Ifrah Hameed, Sabahat Ali, Aqsa Abbasi, Aleza Moqaddas, Nimra Asghar, Abdul Azeez Khan, Owais Aziz, Muhammad Sabtain Khan, Hafsa Razzaq, & Hafiz Muhammad Haseeb Khaliq. (2024). INSIGHTS FROM CRISPR-CAS TECHNOLOGY: REVOLUTIONIZING CANCER RESEARCH, UNVEILING THERAPEUTIC STRATEGIES, AND ADVANCING PERSONALIZED CANCER TREATMENT APPROACHES. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 296–317. https://doi.org/10.53555/jptcp.v31i8.7303
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Author Biographies
Zainab Kalsoom
Department of Biosciences, COMSATS university Islamabad, Pakistan
Warda Javed
Department of Botany, Government College University Faisalabad Pakistan
Sarshar Sannam
Department of Biosciences, COMSATS university Islamabad, Pakistan
Muqadas Fatima
Department of Biosciences, COMSATS university Islamabad, Pakistan
Aqsa Manzoor
Department of Biosciences, COMSATS university Islamabad, Pakistan
Durga Devi
Department of Pathology, Bilawal Medical College, Liaquat University of Medical and Health Sciences LUMHS Jamshoro, Pakistan
Ifrah Hameed
Department of Biosciences, COMSATS university Islamabad, Pakistan
Sabahat Ali
Department of Biosciences, COMSATS university Islamabad, Pakistan
Aqsa Abbasi
Department of Biosciences, COMSATS university Islamabad, Pakistan
Aleza Moqaddas
Department of Botany, Mirpur University of Science and Technology MUST, Pakistan
Nimra Asghar
Department of Biosciences, COMSATS university Islamabad, Pakistan
Abdul Azeez Khan
Department of Zoology, KOHAT University of Science and Technology, Pakistan
Owais Aziz
KAM School of Life Sciences, Department of Molecular Pathology and Genomics Forman Christian College (A Chartered University) Lahore, Pakistan
Muhammad Sabtain Khan
Department of Zoology, University of Education, Township Campus Lahore, Pakistan
Hafsa Razzaq
Department of Botany, Mirpur University of Science and Technology MUST, Pakistan
Hafiz Muhammad Haseeb Khaliq
KAM School of Life Sciences Department of Molecular Pathology and Genomics Forman Christian College (A Chartered University) Lahore, Pakistan