USER-FRIENDLY AQUAPATHO KIT FOR RAPID DETECTION OF VIBRIO BACTERIA IN BIOFLOC BASED AQUACULTURE SYSTEMS
Main Article Content
Keywords
Pathogens, Aquapatho kit, Biofloc pond
Abstract
Vibrio infections are a major impediment to the sustainable expansion of the aquaculture industry worldwide. Early detection of vibriosis in aquaculture systems can protect not only fishes but also save economy. A novel Aquapatho kit was engineered for the specific detection of Vibrio bacteria in water samples, utilizing a selective medium that enhances the growth of Vibrio species while inhibiting other bacterial growth. This selective medium facilitates the identification of active Vibrio bacteria by inducing a color change to red/orange. The kit’s efficacy was initially tested using two Vibrio species, Vibrio cholerae and Vibrio parahaemolyticus, along with two negative control bacteria, Escherichia coli and Bacillus cereus. Subsequent validation was conducted on water samples from aquaculture ponds and biofloc-based aquaculture farms. The kit effectively quantified Vibrio concentrations for both tested species in the aquaculture environments, while the negative controls did not elicit a response. Thus, this kit demonstrates high specificity and reliability for detecting Vibrio bacteria in aquaculture settings.
References
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28. Tepaamorndech, S., I. Nookaew, S. M. Higdon, P. Santiyanont, M. Phromson, K. Chantarasakha, W. Mhuantong, V. Plengvidhya and W. Visessanguan. 2020. Metagenomics in bioflocs and their effects on gut microbiome and immune responses in Pacific white shrimp. Fish & shellfish immunology. 106: 733-741.
29. Tubin, J. S. B., S. M. Gutiérrez, M. del Carmen Monroy-Dosta, M. H. Khanjani and M. G. C. Emerenciano. 2023. Biofloc technology and cockroach () insect meal-based diet for Nile tilapia: zootechnical performance, proximate composition and bacterial profile. Annals of Animal Science. 23(3): 877-886.
30. Walker, D. A. U., M. C. M. Suazo and M. G. C. Emerenciano. 2020. Biofloc technology: principles focused on potential species and the case study of Chilean river shrimp Cryphiops caementarius.
31. Xu, W.-J., T. C. Morris and T. M. Samocha. 2016. Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture. 453: 169-175.
32. Xu, W.-J. and L.-Q. Pan. 2013. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture. 412: 117-124.
2. Baiduk, E., S. Popova, A. Karaseva, V. Iarontovskii, A. Neidorf and I. Tkacheva (2023). Biotesting as a modern assessment method of the aquatic environment Biofloc quality. E3S Web of Conferences, EDP Sciences.
3. Baker-Austin, C., J. D. Oliver, M. Alam, A. Ali, M. K. Waldor, F. Qadri and J. Martinez-Urtaza. 2018. Vibrio spp. infections. Nature Reviews Disease Primers. 4(1): 1-19.
4. Bossier, P. and J. Ekasari. 2017. Biofloc technology application in aquaculture to support sustainable development goals. Microbial biotechnology. 10(5): 1012-1016.
5. Burford, M. A., P. J. Thompson, R. P. McIntosh, R. H. Bauman and D. C. Pearson. 2004. The contribution of flocculated material to shrimp (Litopenaeus vannamei) nutrition in a high-intensity, zero-exchange system. Aquaculture. 232(1-4): 525-537.
6. Emerenciano, M. G., A. N. Rombenso, F. d. N. Vieira, M. A. Martins, G. J. Coman, H. H. Truong, T. H. Noble and C. J. Simon. 2022. Intensification of penaeid shrimp culture: an applied review of advances in production systems, nutrition and breeding. Animals. 12(3): 236.
7. Gao, X.-Y., Y. Liu, L.-L. Miao, E.-W. Li, T.-T. Hou and Z.-P. Liu. 2017. Mechanism of anti-Vibrio activity of marine probiotic strain Bacillus pumilus H2, and characterization of the active substance. AMB express. 7: 1-10.
8. García‐Bernal, M., R. Medina‐Marrero, C. Rodríguez‐Jaramillo, O. Marrero‐Chang, Á. Campa‐Córdova, R. Medina‐García and J. Mazón‐Suástegui. 2018. Probiotic effect of Streptomyces spp. on shrimp (Litopenaeus vannamei) postlarvae challenged with Vibrio parahaemolyticus. Aquaculture nutrition. 24(2): 865-871.
9. Gollan, B., G. Grabe, C. Michaux and S. Helaine. 2019. Bacterial persisters and infection: past, present, and progressing. Annual review of microbiology. 73: 359-385.
10. Hostins, B., W. Wasielesky, O. Decamp, P. Bossier and P. De Schryver. 2019. Managing input C/N ratio to reduce the risk of acute hepatopancreatic necrosis disease (AHPND) outbreaks in biofloc systems–a laboratory study. Aquaculture. 508: 60-65.
11. Huang, H.-H., C.-Y. Li, Y. Song, Y.-J. Lei and P.-H. Yang. 2022. Growth performance of shrimp and water quality in a freshwater biofloc system with a salinity of 5.0‰: effects on inputs, costs and wastes discharge during grow-out culture of Litopenaeus vannamei. Aquacultural engineering. 98: 102265.
12. Huang, H.-H., H.-M. Liao, Y.-J. Lei and P.-H. Yang. 2022. Effects of different carbon sources on growth performance of Litopenaeus vannamei and water quality in the biofloc system in low salinity. Aquaculture. 546: 737239.
13. Ju, Z. Y., I. Forster, L. Conquest, W. Dominy, W. C. Kuo and F. David Horgen. 2008. Determination of microbial community structures of shrimp floc cultures by biomarkers and analysis of floc amino acid profiles. Aquaculture Research. 39(2): 118-133.
14. Khanjani, M. H., A. Mohammadi and M. G. C. Emerenciano. 2022. Microorganisms in biofloc aquaculture system. Aquaculture Reports. 26: 101300.
15. Khanjani, M. H., M. T. Mozanzadeh, M. Sharifinia and M. G. C. Emerenciano. 2023. Biofloc: A sustainable dietary supplement, nutritional value and functional properties. Aquaculture. 562: 738757.
16. Khanjani, M. H., M. Sharifinia and S. Hajirezaee. 2022. Recent progress towards the application of biofloc technology for tilapia farming. Aquaculture. 552: 738021.
17. Kumar, S. B., A. H. Shinde, M. J. Behere, D. Italia and S. Haldar. 2021. Simple, rapid and on spot dye-based sensor for the detection of Vibrio load in shrimp culture farms. Archives of Microbiology. 203(6): 3525-3532.
18. Kumar, V., D. V. Nguyen, K. Baruah and P. Bossier. 2019. Probing the mechanism of VPAHPND extracellular proteins toxicity purified from Vibrio parahaemolyticus AHPND strain in germ-free Artemia test system. Aquaculture. 504: 414-419.
19. Kumar, V., S. Roy, B. K. Behera, H. S. Swain and B. K. Das. 2021. Biofloc microbiome with bioremediation and health benefits. Frontiers in microbiology. 12: 741164.
20. Luo, G., J. Xu and H. Meng. 2020. Nitrate accumulation in biofloc aquaculture systems. Aquaculture. 520: 734675.
21. Martínez Cruz, P., A. L. Ibáñez, O. A. Monroy Hermosillo and H. C. Ramírez Saad. 2012. Use of probiotics in aquaculture. International scholarly research notices. 2012
22. Minaz, M., H. Sevgili and İ. Aydın. 2023. Biofloc technology in aquaculture: advantages and disadvantages from social and applicability perspectives. Annals of Animal Science.
23. Nageswari, P., A. K. Verma, S. Gupta, A. Jeyakumari and C. M. Hittinahalli. 2022. Effects of different stocking densities on haematological, non-specific immune, and antioxidant defence parameters of striped catfish (Pangasianodon hypophthalmus) fingerlings reared in finger millet-based biofloc system. Aquaculture International. 30(6): 3229-3245.
24. Nor Azman Kasan, N. A. K., N. A. G. Nurarina Ayuni Ghazali, C. Nurul Fakriah, I. J. Iswadi Jauhari, A. J. Ahmad Jusoh and M. I. Mhd Ikhwanuddin. 2018. 18s rDNA sequence analysis of microfungi from biofloc-based system in Pacific Whiteleg shrimp, Litopenaeus vannamei culture.
25. Putra, I., I. Effendi, I. Lukistyowati, U. M. Tang, M. Fauzi, I. Suharman and Z. A. Muchlisin. 2020. Effect of different biofloc starters on ammonia, nitrate, and nitrite concentrations in the cultured tilapia Oreochromis niloticus system. F1000Research. 9
26. Ramamurthy, T., B. Das, S. Chakraborty, A. K. Mukhopadhyay and D. A. Sack. 2020. Diagnostic techniques for rapid detection of Vibrio cholerae O1/O139. Vaccine. 38: A73-A82.
27. Sanches-Fernandes, G. M., I. Sá-Correia and R. Costa. 2022. Vibriosis outbreaks in aquaculture: addressing environmental and public health concerns and preventive therapies using gilthead seabream farming as a model system. Frontiers in microbiology. 13: 904815.
28. Tepaamorndech, S., I. Nookaew, S. M. Higdon, P. Santiyanont, M. Phromson, K. Chantarasakha, W. Mhuantong, V. Plengvidhya and W. Visessanguan. 2020. Metagenomics in bioflocs and their effects on gut microbiome and immune responses in Pacific white shrimp. Fish & shellfish immunology. 106: 733-741.
29. Tubin, J. S. B., S. M. Gutiérrez, M. del Carmen Monroy-Dosta, M. H. Khanjani and M. G. C. Emerenciano. 2023. Biofloc technology and cockroach () insect meal-based diet for Nile tilapia: zootechnical performance, proximate composition and bacterial profile. Annals of Animal Science. 23(3): 877-886.
30. Walker, D. A. U., M. C. M. Suazo and M. G. C. Emerenciano. 2020. Biofloc technology: principles focused on potential species and the case study of Chilean river shrimp Cryphiops caementarius.
31. Xu, W.-J., T. C. Morris and T. M. Samocha. 2016. Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture. 453: 169-175.
32. Xu, W.-J. and L.-Q. Pan. 2013. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture. 412: 117-124.
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Jul 25, 2024
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USER-FRIENDLY AQUAPATHO KIT FOR RAPID DETECTION OF VIBRIO BACTERIA IN BIOFLOC BASED AQUACULTURE SYSTEMS. (2024). Journal of Population Therapeutics and Clinical Pharmacology, 31(7), 952-962. https://doi.org/10.53555/jptcp.v31i7.7162
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All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Author Biographies
Hira Qaisar
Department of Zoology, Government College Women University Faisalabad, Faisalabad
Dr. Sumaira Aslam
Department of Zoology, Government College Women University Faisalabad, Faisalabad.
Prof. Dr. Robina Farooq
Department of Chemistry, Comsats University, Lahore
Dr. Tamseela Mumtaz
Department of Zoology, Government College Women University Faisalabad, Faisalabad
Dr. Safina Kousar
Department of Zoology, Government College Women University Faisalabad, Faisalabad
How to Cite
USER-FRIENDLY AQUAPATHO KIT FOR RAPID DETECTION OF VIBRIO BACTERIA IN BIOFLOC BASED AQUACULTURE SYSTEMS. (2024). Journal of Population Therapeutics and Clinical Pharmacology, 31(7), 952-962. https://doi.org/10.53555/jptcp.v31i7.7162