VARIATIONS IN GLUCOSE TRANSPORTER GENE SLC2A2 ASSOCIATED WITH DIABETES IN OBESE PATIENTS IN PAKISTANI POPULATION
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Keywords
Diabetes, Insulin, SLC2A2 Gene, MEGA6, Codon Code Aligner, Heterozygous, Obese diabetic patients
Abstract
Diabetes is a hyperglycemic condition caused due to irregular insulin secretion by the beta cells of pancreatic islets. Among types of diabetes, type 2 diabetes mellitus is mainly due to genetic causes. Transport of glucose is major concern in this disease but there is not much research on the glucose transporters. The aim of study was the investigation of genetic variation in gene SLC2A2 responsible for regulation of insulin secretion. Samples of diabetic patients and control group (healthy individuals) were collected from Multan. DNA extraction kit was used for extraction of DNA. Exon 1 amplification of SLC2A2 gene was done by the standard and gradient PCR. Sequencing and further purification procedures were performed by 1st base laboratory, Singapore. Codon Code Aligner was used for the molecular analysis and the sequence alignment of the results was done using software named MEGA6. Results showed the presence of one heterozygous loci at position no. 3995 of gene. This T/G heterozygous loci was observed among diabetic patients. Results conclude that presence of heterozygous position in SLC2A2 gene has association with the high body weight among obese diabetic patients.
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Mehmood Ahmad
Department of Biotechnology and Molecular Biology Virtual University of Pakistan
Dr Mehreen Arshad
Department of pediatric medicine, Ziauddin medical university
Dr Rehmeen Zahid
Nishtar medical University,Multan
Dr Mah Rukh Shaukat
Baqai Medical University
Aisha Ramzan
Government College, University Faisalabad, Pakistan
Samina Alias Naina
Institute of dentistry liaquat university of medical and health sciences, jamshoro
Minahal Fatima
University of Agriculture, Faisalabad, Pakistan
Dr Muhammad Masood Ahmed
Nishtar medical University,Multan
Khawar Ali
Department of Molecular Biology Virtual University of Pakistan,