INVESTIGATING THE PLACENTAL COLLAGEN COMPOSITION IN MATERNAL HEALTHY CONTROLS AND MATERNAL DIABETIC INDIVIDUALS
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Keywords
Maternal diabetes, placenta, collagen deposition, pregnancy complications
Abstract
Background: Maternal diabetes during pregnancy has been associated with various adverse maternal and fetal outcomes. However, the impact of maternal diabetes on collagen deposition in the placenta remains poorly understood. This study investigates the relationship between maternal diabetes and placental collagen content.
Methods: We conducted a case-control study at Nowshera Medical College And Affiliated Hospitals, from July 2022 to June 2023. Fifty pregnant women were enrolled, 25 in the diabetic group and 25 in the non-diabetic (control group). Inclusion criteria included women with a confirmed diagnosis of Type 1 or Type 2 diabetes for the diabetic group and healthy pregnant women for the control group. Exclusion criteria included women with pre-existing chronic conditions other than diabetes or those with pregnancy complications unrelated to diabetes. Maternal age ranged from 18 to 40 years. Placental tissue samples were collected and analyzed histology using Masson's trichrome staining to quantify collagen content. The sample size was calculated based on a power analysis to detect a significant difference in collagen content between groups, considering an effect size of 0.8, a significance level of 0.05, and a power of 0.8. Statistical analysis included independent t-tests and correlation analysis.
Results: The results reveal that maternal diabetes, particularly Type 1 and Type 2, is significantly associated with differences in placental collagen content (mean difference of 0.27 μg/mg, p = 0.003), with a moderate positive correlation observed between maternal age and collagen content (r = 0.62), collectively underscoring the intricate interplay of maternal factors and placental changes in the context of diabetes.
Conclusion: This study demonstrates that maternal diabetes is linked to increased collagen deposition in the placenta. These insights are vital for understanding placental changes in diabetic pregnancies, which could have clinical implications for managing pregnancy complications associated with maternal diabetes.
References
2. Egan AM, Dow ML, Vella A. A review of the pathophysiology and management of diabetes in pregnancy. InMayo Clinic Proceedings 2020 Dec 1 (Vol. 95, No. 12, pp. 2734-2746). Elsevier.
3. Negrato CA, Mattar R, Gomes MB. Adverse pregnancy outcomes in women with diabetes. Diabetology & metabolic syndrome. 2012 Dec;4(1):1-6.
4. Catalano PM. Management of obesity in pregnancy. Obstetrics & gynecology. 2007 Feb 1;109(2 Part 1):419-33.
5. Wan H, Zhang C, Li H, Luan S, Liu C. Association of maternal diabetes with autism spectrum disorders in offspring: a systemic review and meta-analysis. Medicine. 2018 Jan;97(2).
6. Behboudi-Gandevani S, Bidhendi-Yarandi R, Panahi MH, Vaismoradi M. The effect of mild gestational diabetes mellitus treatment on adverse pregnancy outcomes: A systemic review and meta-analysis. Frontiers in endocrinology. 2021 Mar 26;12:640004.
7. Zhu WW, Yang HX, Wang C, Su RN, Feng H, Kapur A. High prevalence of gestational diabetes mellitus in Beijing: effect of maternal birth weight and other risk factors. Chinese medical journal. 2017 May 5;130(09):1019-25.
8. Cindrova-Davies T, Sferruzzi-Perri AN. Human placental development and function. InSeminars in cell & developmental biology 2022 Nov 1 (Vol. 131, pp. 66-77). Academic Press.
9. Liu R, Dai M, Gong G, Chen M, Cao C, Wang T, Hou Z, Shi Y, Guo J, Zhang Y, Xia X. The role of extracellular matrix on unfavorable maternal–fetal interface: focusing on the function of collagen in human fertility. Journal of Leather Science and Engineering. 2022 Apr 15;4(1):13.
10. Whitley GS, Cartwright JE. Cellular and molecular regulation of spiral artery remodelling: lessons from the cardiovascular field. Placenta. 2010 Jun 1;31(6):465-74.
11. Carrasco-Wong I, Moller A, Giachini FR, Lima VV, Toledo F, Stojanova J, Sobrevia L, San Martín S. Placental structure in gestational diabetes mellitus. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2020 Feb 1;1866(2):165535.
12. Farrelly R, Kennedy MG, Spencer R, Forbes K. Extracellular vesicles as markers and mediators of pregnancy complications: Gestational diabetes, pre‐eclampsia, preterm birth and fetal growth restriction. The Journal of Physiology. 2023 Apr 18.
13. Wallace JG, Bellissimo CJ, Yeo E, Fei Xia Y, Petrik JJ, Surette MG, Bowdish DM, Sloboda DM. Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation. Scientific reports. 2019 Nov 26;9(1):17621.
14. Sudamrao Garud M, Anant Kulkarni Y. Hyperglycemia to nephropathy via transforming growth factor beta. Current diabetes reviews. 2014 May 1;10(3):182-9.
15. Attupuram NM, Kumaresan A, Narayanan K, Kumar H. Cellular and molecular mechanisms involved in placental separation in the bovine: A review. Molecular reproduction and development. 2016 Apr;83(4):287-97.
16. Sachidanandam K, Hutchinson JR, Elgebaly MM, Mezzetti EM, Dorrance AM, Motamed K, Ergul A. Glycemic control prevents microvascular remodeling and increased tone in type 2 diabetes: link to endothelin-1. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2009 Apr;296(4):R952-9.
17. Ramlakhan KP, Johnson MR, Roos-Hesselink JW. Pregnancy and cardiovascular disease. Nature Reviews Cardiology. 2020 Nov;17(11):718-31.
18. Bateman BT, Bansil P, Hernandez-Diaz S, Mhyre JM, Callaghan WM, Kuklina EV. Prevalence, trends, and outcomes of chronic hypertension: a nationwide sample of delivery admissions. American journal of obstetrics and gynecology. 2012 Feb 1;206(2):134-e1.
19. Bardos J, Fiorentino D, Longman RE, Paidas M. Immunological role of the maternal uterine microbiome in pregnancy: pregnancies pathologies and alterated microbiota. Frontiers in immunology. 2020 Jan 8;10:2823.
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Dr Night Ara
Associate Professor, Department of Anatomy Nowshera Medical College, Nowshera
Dr Mahvish Javed
Assistant Professor, Department of Anatomy, Khyber Girls Medical College, Peshawar
Dr Shehla Khatoon
Assistant Professor, Department of Anatomy, Khyber Medical College, Peshawar
Dr Waqas Ali
Demonstrator, Department of Anatomy, Nowshera Medical College, Nowshera
Dr Abdul Hafeez Khan
Assistant Professor, Department of Anatomy, Khyber Medical College, Peshawar
Saad Ahmed Idrees
3rd Year MBBS Student, Khyber Medical College, Peshawar