PROXIMATE AND PHYTOCHEMICAL ANALYSIS TO ASSESS THE THERAPEUTIC POTENTIAL OF OCIMUM SANCTUM
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Keywords
Ocimum sanctum, proximate analysis, phytochemical, TPC, TFC, DPPH
Abstract
The potential of medicinal plants to heal chronic illnesses is being investigated more and more. To determine the optimal extraction solvent for phytochemicals and antioxidants from the herb known for its therapeutic properties, Ocimum sanctum leaves were extracted in methanol and then fractionated progressively with n-hexane, ethyl acetate, butanol, and aqueous. Phytochemical and proximate analyses were conducted in this study by using standard reference methods. Using AOAC techniques, the moisture content, total ash, crude fiber, crude protein, and nitrogen-free extract of O. sanctum leaves were determined. Different extracts of O. sanctum leaves were subjected to phytochemical screening for total flavonoid (TFC) and total phenolic content (TPC). Using the DPPH radical scavenging approach, spectrophotometric experimentation was used to assess O. sanctum's antioxidant capability. Results of the proximate analysis revealed that moisture, ash, protein, crude fat, crude fiber, and nitrogen-free were 10.61±0.11, 6.66±0.031, 2.68±0.031, 8.86±0.13, 17.92 ±0.13, and 0.280±0.006, respectively. The TPC concentrations in methanol, n-hexane, ethyl acetate, aqueous, and butanol extracts were 92.37±1.81, 4.85±0.02, 20.80±0.15, 72.19±0.59 and 15.08±0.05, respectively. The TFC concentrations in methanol, n-hexane, ethyl acetate, aqueous, and butanol extract were 67.70±1.04, 3.83±0.03, 14.29±0.42, 54.28±0.90, and 10.17±0.11, respectively. Antioxidant activity of all extracts was shown at a concentration as low as 0.1563 mg/ml. The decreasing order of DPPH scavenging activity expressed by the plant extracts was methanol > aqueous > ethyl acetate > butanol > n-hexane. The results of this study showed that all extracts of O. sanctum had wielded significant antioxidant and phytochemical potential
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Hafiza Sadia Younas
Department of Nutritional Sciences, Government College Women University, Faisalabad
Madiha Ilyas
Department of Nutritional Sciences, Government College Women University, Faisalabad
Faqir Muhammad
Institute of Physiology and Pharmacology, University of Agriculture Faisalabad
Saima Tehseen
Department of Food Science and Technology, Government College Women University, Faisalabad
Ayesha Riaz
Department of Zoology, Government College Women University, Faisalabad