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Abdul Hakeem
Naqeebullah Khan
Rehana Kamal
Attiq-Ur-Rehman Kakar
Habiba Taj
Khair Un Nisa


Flow injection, chemiluminescence, luminol, diperiodatoargentate (DPA), tablets


A simple flow injection-chemiluminescence (FI-CL) method has been developed for the rapid and sensitive detection of paracetamol (PCM) in pharmaceutical formulations. Utilizing luminol-diperiodatoargentate (III) (DPA) system and optimizing all parameters, we attained an effective linearity range of 0.075-0.75 mg L-1, with a coefficient of determination (R2) of 0.9999. The method showed a limit of detection (LOD) of 7 × 10-4 mg L-1, a limit of quantification (LOQ) of 2 × 10-3 mg L-1, and relative standard deviations (RSDs) ranging from 0.3-1.1%, which indicated high precision. Furthermore, the method achieved a high injection throughput of 120 h-1, which showed it was suitable for real-world applications. Validation through comparison with a reported FI-CL method using the paired Student's t-test shown no significant differences at the 95% confidence level, and the method was successfully applied to determine PCM in pharmaceutical tablets.

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