BIOLOGICAL AND GAS CHROMATOGRAPHY MASS SPECTROMETRY STUDIES ON EXTRACT AND FRACTIONS OF IPHIONIA GRANTIOIDES
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Keywords
Iphionia grantioides, Extraction, Fractionation, Antileishmanial,, Antibacterial, Antifungal, Anticancer, Anti-inflammatory, BSLA, GC-MS
Abstract
In the health system, the demand for different plants as medicinal plants, has achieved key importance all over the world in the case of both plants and humans. In this research study, the biological activities for instance Antileishmanial, antibacterial, antifungal, anticancer (HeLa, 3T3, and PC3 cell lines), anti-inflammatory, and Brine shrimp lethality assay and Gas chromatography mass spectrometry are studied. Antileishmanial bioassay, microplate alamar blue assay, agar tube dilution method, MTT assay, oxidative burst assay using chemiluminescence technique, β-hatching techniques and Triple quadrupole acquisition method MS parameters are the methods used for Antileishmanial, antibacterial, antifungal, anticancer, anti-inflammatory, Brine shrimp lethality assay and Gas chromatography mass spectrometry. one extract for instance whole plant methanol extract of Iphionia grantioides (WMEIG) and two fractions such as whole plant n-hexane fraction of Iphionia grantioides (WHFIG), whole plant aqueous fraction of Iphionia grantioides (WAFIG) extracted and fractionated from the medicinal plant of Baluchistan, Iphionia grantioides. None of the extracts and fractions of Iphionia grantioides showed Antileishmanial activities and having IC50 above 100. The extract and fractions of Iphionia grantioides were inactive against bacterial strains. All extract and fractions of Iphionia grantioides exhibited no antifungal activities none of the extract and fractions of Iphionia grantioides showed anticancer activities against HeLa, PC3 and 3T3 cell lines. None of the extracts and fractions of Iphionia grantioides showed anti-inflammatory activities and lethality. GC-MS studies revealed that whole plant methanol extract of Iphionia grantioides (WMEIG) consists of 16 compounds, whole plant n-hexane fraction of Iphionia grantioides (WHFIG) consists of 13 compounds, and whole plant aqueous fraction of Iphionia grantioides (WAFIG) consists of 25 compounds.
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Farzana Kamalan
Department of Chemistry, University of Turbat Kech, 92600 Balochistan, Pakistan
Jahangir Khan Achakzai
Discipline of Biochemistry, Department of Natural and Basic Sciences, University of Turbat Kech, 92600 Balochistan, Pakistan
Muhammad Anwar Panezai
Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan
Basira Akhtar
Department of Botany, University of Balochistan, Quetta 87300, Pakistan
Abdul Raoof
Agriculture Research Institute, Sariab Quetta, Pakistan
Ali Akbar
Centre for Biotechnology and Microbiology (CBM) University of Swat, Swat 19130 Khyber Pakhtun Khwa.
Shahabuddin Kakar
Department of Zoology, University of Balochistan, Quetta 87300, Pakistan
Nisar Ahmed Shahwani
Faculty of Pharmacy, University of Balochistan, Quetta 87300, Pakistan
Javed Khan
Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan
Nazima Yousaf Khan
Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan
Ghulam Mustafa Khan
Department of Chemistry, University of Balochistan, Quetta 87300, Pakistan
Nizam Baloch
Department of Chemistry, University of Balochistan, Quetta 87300, Pakistan
Bakht Zareen Rahim
Department of Botany, University of Balochistan, Quetta 87300, Pakistan
Noor Hassan
Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan
Abdul Manan Kakar
Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan