ALTERATIONS IN THE GUT MICROBIOME OF INDIVIDUALS WITH TUBERCULOSIS OF DIFFERENT DISEASE STATES

Main Article Content

Mohammad Israr
Aziz Ur Rehman
Madeeha Jadoon
Ayesha Naureen Awan
Nadia Halem
Sofia Shoukat

Keywords

Gut Microbiome, Pulmonary tuberculosis, latent tuberculosis infection, healthy control

Abstract

Purpose: Alterations in the Gut Microbiome of Individuals with Tuberculosis of Different Disease States


Place of study: This study was conducted at the Biochemistry Department of Bacha Khan Medical College, Mardan from January 2019 to January 2020.


Methods and Materials: This study was conducted at the Biochemistry Department of Bacha Khan Medical College, Mardan from January 2019 to January 2020. A total of 100 subjects were enrolled and categorized into three groups: Pulmonary tuberculosis (PTB), latent tuberculosis infection (LTBI), and healthy controls (HC).


Results: Significant differences in gut microbial diversity were observed across the study groups, with PTB patients exhibiting lower alpha diversity compared to LTBI and HC groups (p < 0.001). PCoA revealed distinct clustering of samples based on disease states, and PERMANOVA confirmed the significant impact of grouping variables on microbiota composition (p = 0.001). LEfSe analysis identified 27 genera showing differential abundance between groups, with Bifidobacterium and Bacteroides being more abundant in PTB. A random forest classifier model using the top 10 differential genera achieved high accuracy in discriminating between PTB, LTBI, and HC groups (average accuracy ranging from 80.72% to 86.30%).


Conclusion: In conclusion, this study demonstrated significant dysbiosis of the gut microbiota in active pulmonary tuberculosis patients compared to latent tuberculosis infection and healthy controls based on diversity and compositional alterations.

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