Enhancing Protein Solubility and Functionality: The receptor of the Egg Jelly Domain of Polycystin-1 protein
Main Article Content
Keywords
Polycystin-1, Receptor of the egg jelly (REJ) domain, Phosphoramidite synthetic method
Abstract
The polycystic kidney disease (PKD-1) gene produces the polycystin-1 (PC-1) protein, which includes a substantial extracellular region housing multiple functional polypeptide motifs. In this study, one of the domains of interest is the receptor of the egg jelly (REJ). In the field of proteomics research, generating recombinant proteins in their native, soluble state and in significant quantities often presents a substantial challenge. To address these challenges in expression, we employed expression vectors that incorporate a distinct affinity fusion tag, the maltose binding protein (MBP), to facilitate the production of the REJ fusion protein in a soluble form. MBP serves as a molecular chaperone, thereby aiding in the accurate folding of the fusion protein. Our data indicate that the inclusion of the MBP tag significantly improved both the yield and solubility of the REJ protein. The results obtained from our study provide an experimental approach for the further exploration of solubility, functionality, and interactions of REJ with extracellular matrix (ECM) proteins. Moreover, this approach lays the groundwork for a systematic investigation into the impact of disease-causing mutations within the REJ module of human PC-1, with the potential to greatly enhance the overall production of fused proteins.
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Greenfield, E.A., DeCaprio, J., & Brahmandam, M. (2020). Preparing GST-, His-, or MBP-fusion proteins from bacteria. Cold Spring Harbor Protocols, (9), pdb.prot100024. https://doi.org/10.1101/pdb.prot100024.
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Grund, M.E., Soo, J., Cote, C.K., Berisio, R., & Lukomski, S. (2021). Thinking Outside the Bug: Targeting Outer Membrane Proteins for Burkholderia Vaccines. Cells, 10 (3), 495. https://doi.org/10.3390/cells10030495.
Gunaratne, H. J., Moy, G. W., Kinukawa, M., Miyata, S., Mah, S. A., & Vacquier, V. D. (2007). The 10 sea urchin receptor for egg jelly proteins (SpREJ) are members of the polycystic kidney disease-1 (PKD1) family. BMC genomics, 8, 235. https://doi.org/10.1186/1471-2164-8-235.
Guo, Y., Yu, M., Jing, N., & Zhang, S. (2018). Production of Soluble Bioactive Mouse Leukemia Inhibitory Factor from Escherichia Coli Using MBP Tag. Protein Expression and Purification, 150, 86–91. https://doi.org/10.1016/j.pep.2018.05.006
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Apr 04, 2024
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Aljazi Abdullah AlRashidi, Hala Salim Sonbol. (2024). Enhancing Protein Solubility and Functionality: The receptor of the Egg Jelly Domain of Polycystin-1 protein. Journal of Population Therapeutics and Clinical Pharmacology, 31(2), 1084-1094. https://doi.org/10.53555/jptcp.v31i2.5358
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How to Cite
Aljazi Abdullah AlRashidi, Hala Salim Sonbol. (2024). Enhancing Protein Solubility and Functionality: The receptor of the Egg Jelly Domain of Polycystin-1 protein. Journal of Population Therapeutics and Clinical Pharmacology, 31(2), 1084-1094. https://doi.org/10.53555/jptcp.v31i2.5358