Molecular Detection of Rotavirus and Coronavirus in Potable Water Samples of District Faisalabad
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Keywords
Potable water, Prevalence; PCR; Sewage; Ground water, Filtered; Virus.
Abstract
Rotavirus causes gastroenteritis in infants and young children, and it has been distributed worldwide. Coronavirus is common all around the world. It infects the respiratory tract of both humans and animals.
Methods: To explore the presence of rotavirus and corona virus in water, 50 potable water samples were collected from different areas of Faisalabad, Pakistan. Sewage water, ground water and filtered water samples were collected in sterilized falcon tubes and transported to the lab under refrigerated conditions. RNA was extracted from the water samples using the kit. Extracted viral RNA was used as a template for the synthesis of cDNA using oligoprimers against conserved regions of both Rotavirus and Coronavirus. RT-PCR was done, and gel electrophoresis was performed. Results: PCR results of rotavirus gave product size of 981 bps. Rotavirus was detected in 42 % (21) samples and in the case of coronavirus all the samples were negative. Results indicated that only rotavirus is prevalent in Faisalabad.
Conclusion: This study indicated that rotavirus was prevalent in the potable water of Faisalabad; its presence was higher in sewage water and lower in ground water. Coronavirus was not present in the water samples of Faisalabad.
References
Alhazmi A. Challenges presented by MERS corona virus, and SARS corona virus to global health. Saudi journal of biological sciences, (2016); 23:507-511.
Alosail AM, Alwazzah MJ. The history and epidemiology of Middle East respiratory syndrome corona virus. Multidisciplinary respiratory medicine. (2017); 12:20-26.
Drexler J.F, Corman VM, Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antiviral research. (2014); 101:45–56.
Hassine ZM, Kaplon J, Salem IB, Loulizi KS, Sakly N, Pothier P, Aouni M, Balay KA. Detection and genotyping of group A rotaviruses isolated from sewage samples in Monastir, Tunisia between April 2007 and April 2010. Journal of applied microbiology, (2015); 119:1443-1453.
Izzo MM, Kirkland PD, Gu X, Lele Y, Gunn AA, House JK. Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf feces in Australia. Australian veterinary journal, (2012); 90:122-129.
Jiehao C, Jing X, Daojiong L, Lei X, Zhenghai Q, Yuehua Z, Hua Z, Xiangshi W, Yanling G, Aimei X, He T, Hailing C, Chuning W, Jingjing L, Jianshe W, Mei Z. A Case Series of children with 2019 novel coronavirus infection: clinical and epidemiological features. Clinical Infectious Diseases, (2019); 71:1547–1551
Mast T.C, Chen P, Lu K, Hsu C, Chuan H, Liao W, Lin D, Chen H, Lac C. Epidemiology and economic burden of rotavirus gastroenteritis in hospitals and paediatric clinics in Taiwan , 2005 – 2006. Vaccine, (2010); 28 :3008–3013.
Masters PS. The molecular biology of coronaviruses. Advances in virus research, (2006); 66:193-292.
Moresco V, Damazo NA, Barardi CRM. Rotavirus vaccine stability in the aquatic environment. Journal of applied microbiology, (2015); 120:321–328.
Mukaratirwa A, Berejena C, Nziramasanga P, Ticklay I, Gonah A, Nathoo K, Manangazira P, Mangwanya D, Marembo J, Mwenda JM, Weldegebriel G, Seheri M, Tate JE, Yen C, Parashar U, Mujuru H. Distribution of rotavirus genotypes associated with acute diarrhoea in Zimbabwean children less than five years old before and after rotavirus vaccine introduction. Vaccine. (2018); 36:7248–7255.
Mursalova N, Shugayev N, Suleymanova J, Daniels DS, Wasley A, Cohen AL, Aliabadi N. Rotavirus gastroenteritis surveillance in Azerbaijan , 2011 – 2016. Vaccine. (2018); 36 :7790–7793.
Narayanan K, Ramirez SI, Lokugamage KG, Makino S. Coronavirus nonstructural protein 1: Common and distinct functions in the regulation of host and viral gene expression. Virus research, (2015); 202 :89–100.
Rayamajhi A, Thapa A, Kumar M, Yen C, Tate JE, Parashar UD, Karki A. Preparing for rotavirus vaccine introduction – A retrospective assessment of the epidemiology of intussusception in children below 2 years of age in Nepal. Vaccine. (2018); 36:7836–7840.
Sadiq A, Yinda KC. Rotavirus : Genetics , pathogenesis and vaccine advances. Reviews in medical virology, (2018); 6 :e2003.
Salim H, Karyana IPG, Sanjaya-Putra IGN, Budiarsa S, Soenarto Y. Risk factors of rotavirus diarrhea in hospitalized children in Sanglah Hospital, Denpasar: A prospective cohort study. BMC Gastroenterology, (2014); 14:2–7.
Ul S, Bhutto A, Ma S, Ul M, Bhutto A. Water Quality Assessment in Sindh , Pakistan : A Review. Open Access Journal of Environmental Soil Science, (2019); 3 :296–302
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