Identification of phages against Clostridium perfringens isolated from diarrhea cases of Humans
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Keywords
BHI, Clostridium perfringens, RCM
Abstract
Clostridium perfringens is a gram positive, rod shaped, anaerobic and spore forming bacteria. It causes diarrhea among humans which lead towards the severe health and economic losses worldwide. It is also an important pathogen which causes the food born and gas gangrene illness in both humans and animals. Alternative therapies are required for the cure of the bacterial infections because of the increase in antimicrobial resistance in bacteria. Now a day’s bacteriophages play a key role for the curing of bacterial infections that are resistant to antibiotics.
Aim of the study: The objective of this study was to isolate Clostridium perfringens from the clinical cases of diarrhea from individuals and to identify bacteriophages against the diarrhea causing Clostridium perfringens strain. Materials and methods: A total of 10 feces samples were collected from the diarrhea clinical cases of different individuals from Allied Hospital Faisalabad. RCM, BHI and 5% defibrinate sheep blood agar were used for Clostridium perfringens isolation and the biochemical tests were performed for further confirmation of the bacteria. Bacteriophages were isolated from the diarrhea cases of humans and its lytic activity was examined against the Clostridium perfringens through the spot test technique and agar overlay method. Results: Out of these 10 clinical specimens, Clostridium perfringens was isolated in 6(60%) samples. It showed blackening in RCM broth, turbidity in BHI broth and beta hemolysis occurred in Blood agar. Conclusion: Routine periodic sampling of faeces would facilitate the selection of appropriate method. Clostridium perfringens were isolated and bacteriophages were identified against it
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