Effective biotransformation of benzaldehyde to a stable L-PAC using calcium alginate entrapped pyruvate decarboxylase of an auxotrophic Saccharomyces cerevisiae
Main Article Content
Keywords
Saccharomyces cerevisiae, L-phenylacetylcarbinol, Biotransformation, Benzaldehyde, Calcium alginate entrapment
Abstract
The present study deals with the effective biotransformation of benzaldehyde to a stable L-PAC using calcium alginate entrapped pyruvate decarboxylase of an auxotrophic Saccharomyces cerevisiae via submerged fermentation. The wild-type of Saccharomyces cerevisiae ISL-7 was mutagenised using UV radiations. Significant increase in L-PAC production was found as compared to wild-type. Out of 29 mutants, UV-t6 was selected for maximum L-PAC production after primary and secondary screening. The auxotrophic mutant UV-t6 resulted in 1.25fold increase in L-PAC activity than wild-type ISL-7 when exposed to UV radiations. The effect of different benzaldehyde concentrations and incubation time on production of L-PAC was also studied. The maximum PDC activity 95.99±5.7 U/ml and L-PAC activity 22.46±1.34 g/l was observed after 4 h of incubation at 28ºC for 60 µl benzaldehyde concentration. The selected mutant strain UV-t6also showed maximum L-PAC production with better L-PAC fermentation kinetics as compared to wild-type ISL-7. The PDC of auxotrophic mutant strain of Saccharomyces cerevisiae UV-t6 was immobilized using calcium alginate beads. The effect of enzyme concentration, bead size and cell holding time on L-PAC activity was also studied. The immobilized enzyme exhibited maximum PDC and L-PAC activity i.e. 116.22±8.13 U/ml and 13.72±0.96g/l at enzyme concentration 1.5 ml as compared to free enzyme (PDC activity 115.20±8.06 U/ml and L-PAC activity 12.97±0.91 g/l). The maximum L-PAC activity was observed for bead size 3 mm i.e. 14.56±1.16 g/l along with cell holding time 10 min. This study revealed that auxotrophic mutant strain of Saccharomyces cerevisiae along with immobilized PDC exhibited 1.68fold increase in L-PAC production than wild-type.
References
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Bakhtawar Khan, Sikander Ali, Hafsa Ahmad, Saba Nasir, Arslan Younas, Yousaira Masud, Rahat Zamir, Namra Mariam, Nishat Zafar. (2024). Effective biotransformation of benzaldehyde to a stable L-PAC using calcium alginate entrapped pyruvate decarboxylase of an auxotrophic Saccharomyces cerevisiae. Journal of Population Therapeutics and Clinical Pharmacology, 31(2), 884-913. https://doi.org/10.53555/jptcp.v31i2.4847
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How to Cite
Bakhtawar Khan, Sikander Ali, Hafsa Ahmad, Saba Nasir, Arslan Younas, Yousaira Masud, Rahat Zamir, Namra Mariam, Nishat Zafar. (2024). Effective biotransformation of benzaldehyde to a stable L-PAC using calcium alginate entrapped pyruvate decarboxylase of an auxotrophic Saccharomyces cerevisiae. Journal of Population Therapeutics and Clinical Pharmacology, 31(2), 884-913. https://doi.org/10.53555/jptcp.v31i2.4847