The Effect Of Glucose, Temperature And Ph On Bioethanol Production By Saccharomyces Cerevisiae

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Maria Abdul Salam, Abida Mushtaque, Sara khan, Qurat-ul-Ain, Arslan Younas, Nadia Fatima, Aneela Younas Malik, Mahtab Ali Shah, Humsa Khan


Saccharomyces cerevisiae, Ethanol, Fermentation, Glucose, Biomass


The study used Saccharomyces cerevisiae to ferment sugar to produce ethanol, examining its ability to grow and ferment glucose at different temperatures and pH. Temperature, substrate concentration, and pH all impacted ethanol fermentation process. The system showed high rates of ethanol production and cell growth at 30-45°C, with maximum sugar conversion at 30°C. The optimal pH range for ethanol production was 4.0 to 5.0, with the highest specific ethanol production rate at 120 mg/g.h at pH 5.0. the level of oxygen concentration allowed yeast to use ethanol as a carbon source, unlike in the absence of oxygen. The findings demonstrated that S. cerevisiae could grow and ferment glucose at high temperatures, however the efficiency of the fermentation dropped as the temperature rose over a specific point. Additionally, up to a certain point, greater substrate concentrations enhanced ethanol production until the yield stop increasing. With an ideal pH range being identified for maximal ethanol production, pH also played a significant role in the fermentation of ethanol. According to these results, S. cerevisiae-based ethanol fermentation may be considerably improved by controlling temperature, substrate concentration, and pH.

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