The Effect Of Glucose, Temperature And Ph On Bioethanol Production By Saccharomyces Cerevisiae
Main Article Content
Keywords
Saccharomyces cerevisiae, Ethanol, Fermentation, Glucose, Biomass
Abstract
The study used Saccharomyces cerevisiae to ferment sugar to produce ethanol, examining its ability to grow and ferment glucose at different temperatures and pH. Temperature, substrate concentration, and pH all impacted ethanol fermentation process. The system showed high rates of ethanol production and cell growth at 30-45°C, with maximum sugar conversion at 30°C. The optimal pH range for ethanol production was 4.0 to 5.0, with the highest specific ethanol production rate at 120 mg/g.h at pH 5.0. the level of oxygen concentration allowed yeast to use ethanol as a carbon source, unlike in the absence of oxygen. The findings demonstrated that S. cerevisiae could grow and ferment glucose at high temperatures, however the efficiency of the fermentation dropped as the temperature rose over a specific point. Additionally, up to a certain point, greater substrate concentrations enhanced ethanol production until the yield stop increasing. With an ideal pH range being identified for maximal ethanol production, pH also played a significant role in the fermentation of ethanol. According to these results, S. cerevisiae-based ethanol fermentation may be considerably improved by controlling temperature, substrate concentration, and pH.
References
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Tian S, Li Y, Wang Z, Yang X (2013) Evaluation of Simultaneous Saccharification and Ethanol Fermentation of Undetoxified Steam-Exploded Corn Stover by Saccharomyces cerevisiae Y5. Bioenergy Res 6:1142–1146. https://doi.org/10.1007/s12155-013-9296-5
Tse TJ, Wiens DJ, Reaney MJT (2021) Production of bioethanol—a review of factors affecting ethanol yield. Fermentation 7
Ylitervo P, Franzén CJ, Taherzadeh MJ (2011) Ethanol production at elevated temperatures using encapsulation of yeast. J Biotechnol 156:22–29. https://doi.org/10.1016/j.jbiotec.2011.07.018
Zhao L, Zhang X, Xu J, et al (2015) Techno-economic analysis of bioethanol production from lignocellulosic biomass in china: Dilute-acid pretreatment and enzymatic hydrolysis of corn stover. Energies (Basel) 8:4096–4117. https://doi.org/10.3390/en8054096
Aldiguier AS, Alfenore S, Cameleyre X, et al (2004) Synergistic temperature and ethanol effect on Saccharomyces cerevisiae dynamic behaviour in ethanol bio-fuel production. Bioprocess Biosyst Eng 26:217–222. https://doi.org/10.1007/s00449-004-0352-6
Alriksson B, Cavka A, Jönsson LJ (2011) Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents. Bioresour Technol 102:1254–1263. https://doi.org/10.1016/j.biortech.2010.08.037
Branco RHR, Serafim LS, Xavier AMRB (2019) Second generation bioethanol production: On the use of pulp and paper industry wastes as feedstock. Fermentation 5
Broda M, Yelle DJ, Serwańska K (2022a) Bioethanol Production from Lignocellulosic Biomass—Challenges and Solutions. Molecules 27
Bušić A, Mardetko N, Kundas S, et al (2018) Bioethanol production from renewable raw materials and its separation and purification: A review. Food Technol Biotechnol 56:289–311
Chander Kuhad R, Mehta G, Gupta R, Sharma KK (2010) Fed batch enzymatic saccharification of newspaper cellulosics improves the sugar content in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae. Biomass Bioenergy 34:1189–1194. https://doi.org/10.1016/j.biombioe.2010.03.009
De Bari I, Cuna D, Di Matteo V, Liuzzi F (2014) Bioethanol production from steam-pretreated corn stover through an isomerase mediated process. N Biotechnol 31:185–195. https://doi.org/10.1016/j.nbt.2013.12.003
Ezzat D, Soliman M, Ahmed E, Hassanien AE (2023) An optimized explainable artificial intelligence approach for sustainable clean water. Environ Dev Sustain. https://doi.org/10.1007/s10668-023-03712-0
Gao C, Fleet GH (1988) The effects of temperature and pH on the ethanol tolerance of the wine yeasts, Saccharomyces cerevisiae, Candida stellata and Kloeckera apiculata
Hashmi M, Shah AA, Hameed A, Ragauskas AJ (2017) Enhanced production of bioethanol by fermentation of autohydrolyzed and C4mimOAc-Treated sugarcane bagasse employing various yeast strains. Energies (Basel) 10:. https://doi.org/10.3390/en10081207
Jeevan Kumar SP, Sampath Kumar NS, Chintagunta AD (2020) Bioethanol production from cereal crops and lignocelluloses rich agro-residues: prospects and challenges. SN Appl Sci 2
Karagöz P, Özkan M (2014) Ethanol production from wheat straw by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture in batch and continuous system. Bioresour Technol 158:286–293. https://doi.org/10.1016/j.biortech.2014.02.022
Kim SK, Park DH, Song SH, et al (2013) Effect of fermentation inhibitors in the presence and absence of activated charcoal on the growth of Saccharomyces cerevisiae. Bioprocess Biosyst Eng 36:659–666. https://doi.org/10.1007/s00449-013-0888-4
Lin TS, Kheshgi HS, Song Y, et al (2023) Which crop has the highest bioethanol yield in the United States? Front Energy Res 11:. https://doi.org/10.3389/fenrg.2023.1070186
Lin Y, Zhang W, Li C, et al (2014) Factors affecting ethanol fermentation using Saccharomyces cerevisiae BY4742. Biomass Bioenergy 47:395–401. https://doi.org/10.1016/j.biombioe.2012.09.019
Ludwig D, Amann M, Hirth T, et al (2013) Development and optimization of single and combined detoxification processes to improve the fermentability of lignocellulose hydrolyzates. Bioresour Technol 133:455–461. https://doi.org/10.1016/j.biortech.2013.01.053
Lugani Y, Rai R, Prabhu AA, et al (2020) Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts. Biofuel Research Journal 7:1267–1295. https://doi.org/10.18331/BRJ2020.7.4.5
Murillo-Alvarado PE, Flores Russell E (2022) Optimization approach for bioethanol production from agro-industrial waste. Front Energy Res 10:. https://doi.org/10.3389/fenrg.2022.975133
Parawira W, Tekere M (2011) Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: Review. Crit Rev Biotechnol 31:20–31
Sanni A, Olawale AS, Sani YM, Kheawhom S (2022) Sustainability analysis of bioethanol production from grain and tuber starchy feedstocks. Sci Rep 12:. https://doi.org/10.1038/s41598-022-24854-7
Sebayang AH, Masjuki HH, Ong HC, et al (2016) A perspective on bioethanol production from biomass as alternative fuel for spark ignition engine. RSC Adv 6:14964–14992. https://doi.org/10.1039/c5ra24983j
Shen Y, Guo JS, Chen YP, et al (2012) Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium. J Biotechnol 160:229–235. https://doi.org/10.1016/j.jbiotec.2012.02.008
Singh A, Bajar S, Bishnoi NR (2014) Enzymatic hydrolysis of microwave alkali pretreated rice husk for ethanol production by Saccharomyces cerevisiae, Scheffersomyces stipitis and their co-culture. Fuel 116:699–702. https://doi.org/10.1016/j.fuel.2013.08.072
Tian S, Li Y, Wang Z, Yang X (2013) Evaluation of Simultaneous Saccharification and Ethanol Fermentation of Undetoxified Steam-Exploded Corn Stover by Saccharomyces cerevisiae Y5. Bioenergy Res 6:1142–1146. https://doi.org/10.1007/s12155-013-9296-5
Tse TJ, Wiens DJ, Reaney MJT (2021) Production of bioethanol—a review of factors affecting ethanol yield. Fermentation 7
Ylitervo P, Franzén CJ, Taherzadeh MJ (2011) Ethanol production at elevated temperatures using encapsulation of yeast. J Biotechnol 156:22–29. https://doi.org/10.1016/j.jbiotec.2011.07.018
Zhao L, Zhang X, Xu J, et al (2015) Techno-economic analysis of bioethanol production from lignocellulosic biomass in china: Dilute-acid pretreatment and enzymatic hydrolysis of corn stover. Energies (Basel) 8:4096–4117. https://doi.org/10.3390/en8054096
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Published
Jan 22, 2024
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How to Cite
Maria Abdul Salam, Abida Mushtaque, Sara khan, Qurat-ul-Ain, Arslan Younas, Nadia Fatima, Aneela Younas Malik, Mahtab Ali Shah, Humsa Khan. (2024). The Effect Of Glucose, Temperature And Ph On Bioethanol Production By Saccharomyces Cerevisiae . Journal of Population Therapeutics and Clinical Pharmacology, 31(1), 656–667. https://doi.org/10.53555/jptcp.v31i1.4174
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