SCREENING OF THE ANTI-PROLIFERATIVE ACTIVITY OF Polyscias fruticosa (L) HARMS LEAF AgNPs ON LUNG CANCER CELL LINE

Abstract

The present work focuses on the acute toxicity and anti-proliferative studies of Polyscias fruticosa silver nanoparticles (Pfl AgNP) prepared from its leaf saponin extract (Pfls). The prepared nanoparticles are evaluated by particle size measurement, zeta potential, UV analysis and SEM studies. P. fruticosa growing in India included in the same family of ginseng (Araliaceae), contain large amounts of triterpenoid saponins in their leaves and roots. The Araliaceae family members are known for various therapeutic properties like adaptogenic, immunostimulant, antioxidant activities etc, as evidenced from the bioactivities of ginseng, included in the same family and the therapeutic efficacy is well explored.

The results indicated that the prepared silver nanoparticles laid in the nanoparticle range (100-200nm) and exhibited characteristic zeta potential values, and SEM data. The Pfl AgNP showed UV λ_max at 420 nm. The LC₅₀ was found to be 641mcg/ml & more than 1000mcg /ml for Pfl AgNP and Pfls respectively in Brine shrimp assay method. The anticancer activity is screened on lung cancer cell lines (A549) followed by MTT assay. The anticancer activity studies indicate that the Pfl AgNP at 125 mcg concentration (54.25% cytotoxicity) with reference to the standard etoposide at 50 mcg concentration (65.34 % cytotoxicity).