EVALUATION OF THE CYTOTOXIC AND ANTI-CANCER ACTIVITIES OF POLYSCIAS FRUTICOSA (L) HARMS ROOT PHYTOSOME ON NEUROBLASTOMA CELL LINE

Main Article Content

Madhu. C. Divakar
G. Shyam Nikethen
Jeffrin.J
Kaviarasan.R
Kulandai Dino. A
S. Shri Vishwapal
K. Nivedha
Sivani Ravindran
Minu.G

Keywords

Polyscias fruticosa, PfrP (Pfr Phytosome), Cytotoxic activity, Anti-cancer studies, neuroblastoma cell lines, MTT assay

Abstract

The present work focuses on the cytotoxic and anti-cancer studies of Polyscias fruticosa phytosome (PfrP) prepared from its root n-butanol extracts. The prepared phytosome is evaluated by particle size measurements, zeta potential and SEM studies. P. fruticosa growing in India included in the same family of ginseng (Araliaceae), contain large amounts of triterpenoid saponins in their leaves and roots. The Araliaceae family members are known for various therapeutic properties like adaptogenic, immunostimulant, antioxidant activities etc, as evidenced from the bioactivities of ginseng, a famous member of this family.


The results indicated that the prepared phytosome laid in the nanoparticle range (50-200nm) and exhibited characteristic zeta potential, and SEM values. Pfr phytosome (40mcg/ml) showed effective cytotoxic action on the Allium cepa root tip cells demonstrated by the presence of cell division abnormalities as compared with standard reference Adriamycin (20mcg/ml). The anticancer activity is screened on neuro blastoma cell lines followed by MTT assay. The anticancer activity studies indicate that the Pfr phytosome at 125 mcg concentration (50.37% cytotoxicity) showed comparable activity with the reference standard etoposide at 50 mcg concentration (62.74 % cytotoxicity).


 

Abstract 290 | pdf Downloads 153

References

1. Divakar MC, Nilani.P, Bensita.M.B. B, and Venkataswamy.K, “A Pharmacognostic report on the leaf and root of Polyscias fruticosa (L) Harms” Ancient Science of Life1998; Vol.18 (2):165- 172. PubMed Central.
2. M.C. Divakar & M.B Bensita. Screening of various leaf extracts of Polyscias fruticosa (L) Harms for their Antidiabetic activity. Indian Journal of Natural Products1998; 14(2): 24-28. H index 14.
3. M.B. Bensita, P. Nilani, S. Sandhya, and M.C. Divakar, Studies on the adaptogenic and antibacterial properties of Polyscias fruticosa (L) Harms. The Ancient Science of Life1999; Vol.18 (3&4):231-246.PubMed Central.
4. Madhu.C. Divakar, Pillai.N.R., and S.B. Rao, Cytotoxic effects of Polyscias fruticosa and Polyscias balfouriana leaf extracts. The Antiseptic2004; 101(5):188-190.
5. M.C. Divakar*, N.R. Pillai and S.B. Rao, Isolation and biological activity studies of two triterpene glycosides from the leaves and roots of Polyscias fruticosa.Ind. J. of Natural Products 2005; 21(3):7-13.H index 18.
6. Madhu.C. Divakar, S. Sheela, Sandhya., Vinod K. R., N. R. Pillai, S. B. Rao, Anti- inflammatory and antioxidant activities of Polyscias saponins. Der Pharmacia letters 2010; 2 (1): 41-47.
7. Dai J, Mumper RJ. Plant active compounds: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313–7352.
8. Vishal Gaurav*, Shivangi Paliwal, Arpita Singh, Swarnima Pandey, Mohd. Aqil Siddiqui. Phytosomes: Preparation, Evaluation and Application. International Journal of Research in Engineering and Science 2021 9 (2) PP. 35-39.
9. Shukla A, Pandey V, Shukla R, Bhatnagar P, Jain S, “Herbosomes’: A Current Concept of Herbal Drug Technology- An Overview”, Journal of Medical Pharmaceutical and Allied Sciences, 2012,1;39-56.
10. Goyal A, Kumar S, Nagpal M, Singh I, Arora S, “Potential of Novel Drug Delivery Systems for Herbal Drugs”, Indian Journal of Pharmaceutical Education and Research, Jul-Sep 2011, 45(3);225- 235.
11. Gandhi A, Dutta A, Pal A, Bakshi P, “Recent Trends of Phytosomes for Delivering Herbal Extract with Improved Bioavailability”, Journal of Pharmacognosy and Phytochemistry, 2012, 1(4);6-14.
12. Kumar D., Vats N., Saroha K., Rana A.C. Phytosomes as Emerging Nanotechnology for Herbal Drug Delivery. In: Saneja A., Panda A.K., Lichtfouse E., editors. Sustainable Agriculture Reviews 43: Pharmaceutical Technology for Natural Products Delivery Vol. 1 Fundamentals and Applications. Springer International Publishing; Cham, Switzerland: 2020. pp. 217–237.
13. Gaikwad A.R., Ahire K.D., Gosavi A.A., Salunkhe K.S., Khalkar A., Gaikwad Abhijeet R. Phytosome as a Novel Drug Delivery System for Bioavailability Enhancement of Phytoconstituents and its Applications: A Review. J. Drug Deliv. Ther. 2021; 11:138–152.
14. Shan L, Tan QY, Hong W, Hong L, Zhang JQ. Preparation, characterization and in vitro anti-tumour activities of evodiamine phospholipids complex. Chin Pharm J. 2012;47(7):517–523.


15. Phytosomes B.S. The new technology for enhancement of bioavailability of botanicals and nutraceuticals. Int J Health Res. 2009;2(3):225–232.
16. Vo Duv Huan, Yamamura S., Ohtani., Kasai R., Chau .H.M (1998) oleanane saponins from Polyscias fruticosa, Phytochemistry, 47(3), 451-57.
17. Basu.N., Rastogi R.P., (1967) Triterpenoid Saponins and Sapogenins, Phytochem 6, 1249-70.
18. Egil Ramstad, Modern Pharmacognosy (1959), Blackiston Division, Mc Graw Hill Book Company, N. York, I Edn, 143-45.
19. Harborne J.B., (1973), Phytochemical Methods, A guide to Modern Techniques of Plant Analysis, Chapman and Hall Ltd., New Fetter Lane London, 1st edn, 116-19, 182-90.
20. Padmaja.V., Jessy S.M., Nair GRN, Nair CRS., Hisham A. (1994) Anti mitotic effects of Uvaria narum and U. hookeri. Fitoterapia 1994; LXV (2):77-81.
21. Santhakumari G., Stephen.J. Anti-mitotic effect of Nimbidin – a first report, Experentia 1981; 37: 91-93.
22. M Igarashi, T Miyazawa. Newly recognized cytotoxic effect of conjugated trienoic fatty acids on cultured human tumour cells. Cancer Lett. 2000 Feb 1;148(2):173-9.