EFFECTIVENESS OF DISCRIMINANT FUNCTIONS AS SCREENING TESTS IN PREDICTING Β THALASSEMIA TRAIT AMONG PAEDIATRIC POPULATION WITH MICROCYTIC ANEMIA
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Keywords
Discriminant functions, microcytosis, Microcytic anemia, BTT
Abstract
Aim: The objective of the present study was to study the effectiveness of various discriminant functions as screening tests in predicting BTT among paediatric population with Microcytic anemia, who were later confirmed by increased HbA2 levels on Agarose gel electrophoresis at alkaline pH (8.6).
Methods: The present study was conducted in the Hematology section Department of Pathology Central Laboratory RRMCH Hospital Bangalore during the period from January 2015 to May 2017. A total number of 300 paediatric patients were included in the present study.
Results: The commonest age group affected was 0-1 year accounting to 48% followed by age group 2-3 year with 22%. Males showed higher preponderance compared to female with M:F ratio [1.4:1]. The prevalence of BTT was 28.33% in the present study. Out of 300 cases 107(35.6%) cases were positive for either both or any one of the indices. All these cases were subjected to electrophoresis. Among these, 85cases showed increased HbA2 levels.
Conclusion: The present study demonstrates that a set of cost-effective screening test like Discriminant Indices along with routine hemogram data in Microcytic Anemia cases can effectively discriminate between BTT and non-BTT. The confirmation of BTT can be done by Hb electrophoresis on agarose gel and HbA2 quantification by automated densitometer scanner.
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Dr. Vara Prasad Bm
Assistant Professor: Ramaiah Medical College, Bangalore.
Dr. Vamseedhar Annam
Professor & HOD, Shri Chamundeshwari Medical College, HOs, Bangalore.
Dr. Vinitra K
Assistant Professor: BGS Global Institute of Medical Sciences , Bangalore.