EVALUATION OF NEUROLOGICAL INVESTIGATION OF ETHANOLIC EXTRACT OF FLOWERS OF NYCTANTHES ARBOR-TRISTIS LINN. BY DIFFERENT PHARMACOLOGICAL MODELS

Main Article Content

Monika Kaushik
Neetesh Kumar Jain

Keywords

Neurological Investigation, Ethanolic Extract, Flowers, Nyctanthes arbor-tristis Linn, Muscle Relaxant Activity, Locomotor Activity

Abstract

AIM- The aim of the present investigation is to evaluate Neurological Investigation of Ethanolic Extract of Flowers of Nyctanthes arbor-tristis Linn. by Different Models. 


MATERIAL & METHODS- The plant material i.e. flowers were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of flower powder of Nyctanthes arbor-tristis were extracted using petroleum ether for the removal of fats and then different solvents were used according to polarity charts. Qualitative chemical tests of different extracts were subjected to various chemical tests to detect various phytoconstituents. The animals were under observation for their behavioral changes if any, at 30 minutes intervals in the first one hour and at the hourly intervals for the next 4 hour for the following parameters. Locomotor activity is easily   measured   using   actophotometer   which   operates   on   photoelectric   cells connected with a counter. When a beam of light falling on the photocell is cut off by the animal a count is recorded and displayed digitally.  The animals were discarded and replaced if they failed to do so. All the groups’ animals were placed on the rota rod and the fall off time from the rotating rod was noted after 60 minutes of test drug and standard administration.


RESULTS- The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds.  Rats treated with ethanolic extract of Nyctanthes arbor-tristis and were submitted to general behavioural profile studies did not show any difference in their behaviors. The ethanolic extract of Nyctanthes arbor-tristis in a dose level of 200 mg/kg, and 400 mg/kg, p.o did not produce statistically any significant reduction in locomotor activity as compared to the control animals receiving only the vehicle. There was statistically significant decrease in the time of falls within 3 minutes after the treatment with ethanolic extract of Nyctanthes arbor-tristis at dose level of 200 mg/kg and 400 mg/kg, p.o and result reveals that Nyctanthes arbor-tristis has no muscle relaxant property. 


CONCLUSION- Nyctanthes arbor-tristis has been demonstrated to ameliorate cognitive processes, not only preventing amnesia induced by pharmacological treatments in elevated plus maze, but also by producing facilitation of social memory in a social learning task which demonstrates that the extract displays memory enhancing properties.

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