FUNCTIONAL CHARACTERIZATION AND PHYTOCHEMICAL PROFILING OF TERMINALIA CHEBULA FRUIT

Main Article Content

Amina Jamil
Imran Pasha
Mian Kamran Sharif
Beenish Israr

Keywords

Terminalia chebula, Composition, FTIR, Fatty acids, Phytochemicals, Antioxidants

Abstract

Terminalia chebula, one of the oldest plants cultivated on the earth, is rich in phytochemicals including phenolics, flavonoids, and organic acids. The present study was designed to analyze Terminalia chebula fruit and their extracts for nutritional composition and therapeutic potential. The proximate analysis indicated that 6.82±0.25%, 4.03±0.13%, 3.40±0.14%, 18.14±0.63%, 5.36±0.40%, 62.20±0.09% moisture, ash content, crude fat, crude fiber, crude protein and NFE, respectively present in Terminalia chebula fruit. The functional properties results revealed that pH of Terminalia chebula fruit was acidic and it exhibited the good swelling power, oil holding and water holding capacity. Terminalia chebula fruit was found to be rich in iron (815.33±10.33 ppm), zinc (33.66±0.3 ppm), manganese (33.81±0.41 ppm) and also have a good amount of copper, cobalt, calcium, magnesium, and potassium. The FTIR results have shown the presence of alcohol, alkane, aldehyde, thiol, amide and alkyl halide functional groups in chebulic fruit powder. Furthermore, the phytochemical and antioxidant properties of Terminalia chebula fruit by using different solvents (Methanol, ethanol and water) were measured and found that methanolic extract showed the higher TPC, TFC and radical scavenging properties.

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References

1. Pfundstein, B., S.K. El Desouky, W.E. Hull, R. Haubner, G. Erben and R.W. Owen. (2010). Polyphenolic compounds in the fruits of Egyptian medicinal plants (Terminalia bellerica, Terminalia chebula and Terminalia horrida): Characterization, quantitation and determination of antioxidant capacities. Phytochemistry, 71(10):1132-48.
https://doi.org/10.1016/j.phytochem.2010.03.018
2. Bag, A., S.K. Bhattacharyya and R.R. Chattopadhyay. (2013). The development of Terminalia chebula Retz. (Combretaceae) in clinical research. Asian Pacific Journal of Tropical Biomedicine. 3(3): 244–252. https://doi.org/10.1016/S2221-1691(13)60059-3
3. Sarala, P. and S.R. Krishnamurthy. (2021). Distribution, Nutritive Value and Mineral Composition of a Few Medicinal Plants of Shimoga District, Karnataka India. International Journal of Pharmaceutical Sciences Review and Research. 69(2): 150-162.
http://dx.doi.org/10.47583/ijpsrr.2021.v69i02.023
4. Basha, S.J., J. Reddy, Y.S. Rani, M. Koshma, G. Hanumanthu and S. Dadakhalandar. (2017). A review on Terminalia chebula. International Journal of Pharmacological Research. 7(10): 187-191 https://doi.org/10.7439/ijpr
5. AOAC, 2016. Official Methods of Analysis. The Association of Official Analytical Chemists. Inc. 20th ed. AOAC Press, Arlington, VA, USA.
6. Segura-Campos, M., E. Barbosa-Martín, A. Matus-Basto, D. Cabrera-Amaro, M. MurguíaOlmedo, M.Y. Moguel-Ordoñez and D. Betancur-Ancona. (2014). Comparison of chemical and functional properties of Stevia rebaudiana (Bertoni) varieties cultivated in Mexican Southeast. American Journal of Plant Sciences. 5(3):286-293.
DOI: 10.4236/ajps.2014.53039
7. Saif, F.A., S.A. Yaseen, A.S. Alameen, S.B. Mane and P.B. Undre. (2021). Identification and characterization of Aspergillus species of fruit rot fungi using microscopy, FT-IR, Raman and UV–Vis spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 246: 119010. https://doi.org/10.1016/j.saa.2020.119010
8. Alam, M.N., N.J. Bristi and M. Rafiquzzaman. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal. 21(2):143-52.
https://doi.org/10.1016/j.jsps.2012.05.002
9. Skotti, E., E. Anastasaki, G. Kanellou, M. Polissiou and P.A. Tarantilis. (2014). Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants. Industrial Crops and Products. 53:46-54.
https://doi.org/10.1016/j.indcrop.2013.12.013
10. Kalita, P., B.K. Tapan, T.K. Pal and R. Kalita. (2013). Estimation of total flavonoids content (tfc) and anti oxidant activities of methanolic whole plant extract of Biophytum sensitivum linn. Journal of Drug Delivery and Therapeutics. 3(4):33-7. https://doi.org/10.22270/jddt.v3i4.546
11. Kumar, V., S.R. Chandel, S. Guleria, N. Sharma, A. Sourirajan, P.K. Khosla and K. Dev. (2021). Comparative analysis of phytochemicals, antimicrobial and antioxidant activity of different species of Terminalia from Himachal Pradesh, India. Vegetos. 34:528-539. https://doi.org/10.1007/s42535-021-00232-y
12. Vemuri. P.K., L. Dronavalli, P. Nayakudugari, A. Kunta and R. Challagulla. (2019) Phytochemical analysis and biochemical characterization of Terminalia chebula extracts for its medicinal use. Biomedical and Pharmacological Journal. 12(3).
https://dx.doi.org/10.13005/bpj/1783
13. Montgomery, D.C. 2017. Design and Analysis of Experiments. 9th Ed. (John Wiley & Sons. Inc. Hoboken, NJ, USA), pp: 162-264.
14. Asati. S, V. Chandel and A. Choubey. (2020). Extraction and comparative study on physico-chemical, phytochemical analysis of fruits of Terminalia chebula and rhizomes of Curcuma longa. Plant Archives. 20: 4289-4294.
15. Hussain, J., A.L. Khan, N. Rehman, M. Hamayun, Z.K. Shinwari, W. Ullah and I. Lee. (2009). Assessment of herbal products and their composite medicinal plants through proximate and micronutrients analyses. Journal of Medicinal Plants Research. 3:1072-1077.
16. Malik, T., V.K. Madan and R. Kumar. (2020). Quantitative estimation of total phenolics content, total flavonoids content, and total antioxidant potential in various promising extracts of Triphala powder and its fruit constituents. Innovations in Pharmaceuticals and Pharmacotherapy. 8(4):105-113. DOI: 10.31690/ipp.2020.v08i04.001
17. Ikram, A., F. Saeed, H. Munir, M.T. Sultan, M. Afzaal, A. Ahmed and F.M. Anjum. (2020). Exploring the amino acid profile and microbial properties of locally sweet preserved kachra hareer (Terminalia chebula). Food Science and Nutrition. 9(2):909-919.
https://doi.org/10.1002/fsn3.2056
18. Salnikow, K. (2021). Role of iron in cancer. Seminars in Cancer Biology. 76:189-194. https://doi.org/10.1016/j.semcancer.2021.04.001
19. Jirankalgikar, Y.M., R.R. Dwivedi, C.R. Harisha and V.J. Shukla. (2012). Assesement of bhavana samskara by phytopharmacognostical evaluation in haritaki churna. International Journal of Ayurveda Allied Sciences. 1:193-197.
20. Pathak, S.S., R.C. Pradhan and S. Mishra. (2019). Physical characterization and mass modeling of dried Terminalia chebula fruit. Journal of Food Process Engineering. 42(3):e12992. https://doi.org/10.1111/jfpe.12992
21. Bhatt, I.D., S. Rawat, A. Badhani and R.S. Rawal. (2017). Nutraceutical potential of selected wild edible fruits of the Indian Himalayan region. Food Chemistry. 215:84-91. https://doi.org/10.1016/j.foodchem.2016.07.143
22. Bulbul, M.R.H., M.N.U. Chowdhury, T.A. Naima, S.A. Sami, M.S. Imtiaj, N. Huda and M.G. Uddin. (2022). A comprehensive review on the diverse pharmacological perspectives of Terminalia chebula Retz. Heliyon. 8(8):e10220. https://doi.org/10.1016/j.heliyon.2022.e10220
23. Kauser, A., S.M.A. Shah, N. Iqbal, M.A. Murtaza, I. Hussain, A. Irshad, S. Nasir, M. Akram, N. Munir and M. Riaz. (2018). In vitro antioxidant and cytotoxic potential of methanolic extracts of selected indigenous medicinal plants. Progress in Nutrition. 20(4):706-712. DOI: 10.23751/pn.v20i4.7523
24. Kanik, B. Singh, J.B. Dhar, G. Jairath, R. Sharma, D. Gopinath, G. Mal. (2021). Antioxidant Potential of Fermented Milk Supplemented with Various Aqueous Herbal Extracts. International Journal of Food Science and Agriculture. 5(4): 762-774. DOI: 10.26855/ijfsa.2021.12.025
25. Saha, S. and R.J. Verma. (2016) Antioxidant activity of polyphenolic extract of Terminalia chebula Retzius fruits. Journal of Taibah University Sciences. 10(6):805-812. https://doi.org/10.1016/j.jtusci.2014.09.003